Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	112,234	A→G	R122R (CGT→CGC)	zapD ←	cell division factor ZapD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	112,234	0	A	G	100.0%	192.5 / NA	52	R122R (CGT→CGC)	zapD	cell division factor ZapD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/13);  total (39/13)

GCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAA  >  NC_000913/112100‑112367                                                                                                                                       |                                                                                                                                      gCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCAc                                                                                                                                  >  2:994916/1‑140 (MQ=255) gCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCAc                                                                                                                                  <  2:191788/140‑1 (MQ=255) gCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCAc                                                                                                                                  >  1:611715/1‑140 (MQ=255)  ccTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCAcc                                                                                                                                 >  1:1536054/1‑140 (MQ=255)   cTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCa                                                                                                                                >  1:307130/1‑140 (MQ=255)    tGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCag                                                                                                                               >  1:490147/1‑140 (MQ=255)     gggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCaga                                                                                                                              >  1:1162589/1‑140 (MQ=255)     gggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCaga                                                                                                                              >  1:1173396/1‑140 (MQ=255)      ggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCagag                                                                                                                             <  1:710193/140‑1 (MQ=255)      ggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCagag                                                                                                                             <  1:609991/140‑1 (MQ=255)      ggTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCagag                                                                                                                             <  2:1398901/140‑1 (MQ=255)        tGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCa                                                                                                                           <  1:844208/140‑1 (MQ=255)          aGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAAt                                                                                                                         >  1:747717/1‑140 (MQ=255)            cGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCa                                                                                                                       >  1:1535522/1‑140 (MQ=255)               gTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAAc                                                                                                                    <  1:290701/140‑1 (MQ=255)                    ggCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCt                                                                                                               <  1:749776/140‑1 (MQ=255)                         gCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACg                                                                                                          >  1:1326099/1‑140 (MQ=255)                                       tACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGa                                                                                            >  2:333926/1‑140 (MQ=255)                                           tGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcg                                                                                        >  1:952471/1‑140 (MQ=255)                                              cTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcgg                                                                                     >  1:668736/1‑140 (MQ=255)                                                gTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcg                                                                                   <  2:1043758/140‑1 (MQ=255)                                                gTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcg                                                                                   <  2:152104/140‑1 (MQ=255)                                                gTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcg                                                                                   <  2:1535522/140‑1 (MQ=255)                                                 tCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgc                                                                                  >  1:1100074/1‑140 (MQ=255)                                                  cgcgCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATAcgcggcgcg                                                                                 <  2:307130/140‑1 (MQ=255)                                                      cTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGaaa                                                                             <  2:611715/140‑1 (MQ=255)                                                               gggTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGc                                                                    >  1:136807/1‑140 (MQ=255)                                                                   aGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTccc                                                                >  1:867784/1‑140 (MQ=255)                                                                    gATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTcccc                                                               >  1:1367689/1‑140 (MQ=255)                                                                      tGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                                                             >  1:637224/1‑140 (MQ=255)                                                                      ggCACCCAAATGGGCAATGTAGTAAAATCAAAGCTGCAACGGCCGCCGGGGAGGCTCAGTCGCGGGCGCCCCAGAGAAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCccgc                                                             <  2:747717/139‑1 (MQ=255)                                                                          gCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCt                                                         >  1:369998/1‑140 (MQ=255)                                                                           ccAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCtt                                                        >  1:350791/1‑140 (MQ=255)                                                                                  tgCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCCCGCAGAAATTGCCCGTTACGCGGCGCGGAAATTAATCCGCTCCCCGCCGCTTTTAActg                                                 <  2:1173396/140‑1 (MQ=255)                                                                                       tGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGaa                                            >  1:409368/1‑140 (MQ=255)                                                                                         tAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAAtt                                          >  2:991754/1‑140 (MQ=255)                                                                                         gaGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCCCCAGAGCAATCAACCGATCTTCCCGCAGAAATTGCCCGATCCGCGGCGCGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAAtt                                          <  2:509412/139‑1 (MQ=255)                                                                                           ggaaaaTCAAAGCTGCAACGGCCGCCTGGGATGCTCGGTGGCTGGCGCCCCGGAGCAATAAACCGATCTCCACGCAGAAATTCCCCGATACGCGGCGCGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                        <  2:1513112/137‑1 (MQ=255)                                                                                           ggTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTaa                                        >  2:1240366/1‑140 (MQ=255)                                                                                            gTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAAt                                       >  2:1151981/1‑140 (MQ=255)                                                                                            gTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAAt                                       >  2:1507621/1‑140 (MQ=255)                                                                                              aaaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGc                                     >  1:1369193/1‑140 (MQ=255)                                                                                               aaTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCTCGCCGCTTTTAACTGCTGAATTAATGCt                                    >  1:590927/1‑140 (MQ=255)                                                                                                aTCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCtt                                   >  1:407350/1‑140 (MQ=255)                                                                                                 tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                  >  1:341946/1‑140 (MQ=255)                                                                                                 tCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTc                                  >  1:717613/1‑140 (MQ=255)                                                                                                   aaaGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCaa                                >  2:1002837/1‑140 (MQ=255)                                                                                                     aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                              <  1:1298423/140‑1 (MQ=255)                                                                                                     aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                              >  2:1074292/1‑140 (MQ=255)                                                                                                     aGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATa                              >  1:842490/1‑140 (MQ=255)                                                                                                      gCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATAc                             >  1:18873/1‑140 (MQ=255)                                                                                                               gccgccTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGt                    >  2:328698/1‑140 (MQ=255)                                                                                                               gccgccGGGGATGCTCAGCCGCTGGCGCCCCAGAGCAATCAAACGTTCTCCCCGCAGAAATTCCCCGATACGCGGCGGGGAAATTAATCCGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGt                    <  2:637224/140‑1 (MQ=255)                                                                                                                cccccggggATGCTCAGTCGCGGGCGCCCCAGAGCAATAAAACGATCTTCAGGCAGAAATTGCCCGATCCGGGGCGGGAAAATTAATCCGCCCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTc                   <  2:327148/134‑1 (MQ=255)                                                                                                                  gccTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCa                 >  1:212252/1‑140 (MQ=255)                                                                                                                   ccTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCAc                >  2:720522/1‑140 (MQ=255)                                                                                                                    cTGGGATGCTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACg               >  2:1236771/1‑140 (MQ=255)                                                                                                                            cTCAGTCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCAc       >  2:759514/1‑140 (MQ=255)                                                                                                                                 tCGCTGGCGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCaa  >  2:1309017/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GCCTGGGTGAGCGGGTTCAGGCTGGCAATCCAGGTTTCTACCTGGCTGTCGCGCTGCGCCTGGGGTAGATGCAGCCAAATGTGCAATGTAGGTAAATCAAAGCTGCAACAGCCGCCTGGGATGCTCAGTCGCTGACGCACCAGAGCAATCAAACGATCTTCACGCAGAAATTGCCCGATACGCGGCGCGGAAATTAATACGCTCCCCGCCGCTTTTAACTGCTGAATTAATGCTTCAATACGGCTCTGGTCCACGCCAGGCACGCCAA  >  NC_000913/112100‑112367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑