Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,633,705	T→C	G28G (GGT→GGC)	pinQ →	Qin prophage; putative site‑specific recombinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,633,705	0	T	C	100.0%	154.6 / NA	70	G28G (GGT→GGC)	pinQ	Qin prophage; putative site‑specific recombinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (56/14);  total (56/14)

TATTTTCTGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGTTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAGCGTCCTGGTTTTAACCGGTTGCTTGCTCGCCTGAAATGTGGTGATCAATTGAT  >  NC_000913/1633569‑1633821                                                                                                                                         |                                                                                                                     tattTTCTGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGcttt                                                                                                                   <  1:1187545/140‑1 (MQ=17)   ttttCTGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGTAGGCTTTaa                                                                                                                 <  1:1176216/140‑1 (MQ=12)        tGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCa                                                                                                            <  1:893844/140‑1 (MQ=17)        tGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCa                                                                                                            >  2:1055957/1‑140 (MQ=17)             tGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCt                                                                                                       <  1:1460755/140‑1 (MQ=17)               cATTATGATATGAACACCAATTTCGTATAGAGTCTCCCTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCa                                                                                                     >  2:898988/1‑140 (MQ=18)                    tgatatgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCaaa                                                                                                >  1:857517/1‑140 (MQ=18)                     gatatgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAaat                                                                                               <  2:1459575/140‑1 (MQ=17)                     gatatgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAaat                                                                                               <  2:1272859/140‑1 (MQ=17)                      atatgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAaata                                                                                              >  1:909966/1‑140 (MQ=17)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  1:953596/1‑140 (MQ=18)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  2:767345/1‑140 (MQ=18)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  1:974616/1‑140 (MQ=17)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  2:1191144/1‑140 (MQ=18)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  2:1299669/1‑140 (MQ=18)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  1:1210982/1‑140 (MQ=18)                         tgaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATc                                                                                           >  1:1096185/1‑140 (MQ=18)                          gaACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCg                                                                                          <  2:1217415/140‑1 (MQ=17)                           aaCACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCga                                                                                         >  1:177398/1‑140 (MQ=17)                           aaCACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCga                                                                                         >  1:174457/1‑140 (MQ=18)                            acacCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGTGAAATCTAAAGTGCAAGCTTTAAAATCAAACCTCAGCAAATAATCgaa                                                                                        >  2:645340/1‑140 (MQ=255)                            acacCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaa                                                                                        >  2:941320/1‑140 (MQ=17)                            acacCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaa                                                                                        >  1:216592/1‑140 (MQ=18)                            acacCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaa                                                                                        <  2:648811/140‑1 (MQ=17)                             ccccAATTTCGTATAGAGTCTCACTATGTCGCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaag                                                                                       <  2:1423299/138‑1 (MQ=12)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTTAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  1:329555/1‑140 (MQ=18)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  2:125391/1‑140 (MQ=17)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  2:1396237/1‑140 (MQ=18)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  2:539182/1‑140 (MQ=18)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  1:970776/1‑140 (MQ=18)                              acCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaaga                                                                                      >  1:220602/1‑140 (MQ=18)                               ccAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCgaagaa                                                                                     <  1:1055957/140‑1 (MQ=17)                                cAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAac                                                                                    >  1:1360360/1‑140 (MQ=18)                                cAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAac                                                                                    >  2:215358/1‑140 (MQ=17)                                  aTTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGGGCAGGGCTTAAAAACAAACCTCAGGAAATAAACGAAGAacac                                                                                  >  2:169938/1‑140 (MQ=11)                                     tCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACAtt                                                                               >  2:1125981/1‑140 (MQ=18)                                      cGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTa                                                                              >  1:276533/1‑140 (MQ=17)                                        tataGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGACGAACACATTAGc                                                                            >  2:658091/1‑140 (MQ=17)                                        tataGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGc                                                                            >  1:777457/1‑140 (MQ=18)                                        tataGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGc                                                                            >  2:1399867/1‑140 (MQ=18)                                          taGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGGGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCgg                                                                          <  1:671607/140‑1 (MQ=17)                                             agTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTc                                                                       >  1:845994/1‑140 (MQ=17)                                             agTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTc                                                                       >  2:960921/1‑140 (MQ=18)                                               tctcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcag                                                                     >  2:540069/1‑140 (MQ=18)                                               tctcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcag                                                                     >  2:540176/1‑140 (MQ=17)                                               tctcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcag                                                                     >  1:431327/1‑140 (MQ=18)                                               tctcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcag                                                                     >  2:1291798/1‑140 (MQ=18)                                               tctcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcag                                                                     >  2:751483/1‑140 (MQ=18)                                                 tcACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagca                                                                   >  2:1544185/1‑140 (MQ=18)                                                  cACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagcag                                                                  >  2:506748/1‑140 (MQ=18)                                                  cACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagcag                                                                  >  2:1454582/1‑140 (MQ=18)                                                  cACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagcag                                                                  <  2:1301693/140‑1 (MQ=17)                                                  cACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagcag                                                                  >  2:1248991/1‑140 (MQ=18)                                                  cACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTcagcag                                                                  <  1:796325/140‑1 (MQ=17)                                                       tGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAAcc                                                             >  1:328133/1‑140 (MQ=18)                                                       tGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAAcc                                                             >  1:597782/1‑140 (MQ=18)                                                       tGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAAcc                                                             >  2:266192/1‑140 (MQ=17)                                                       tGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAAAACATTAGCGGCCCAGCAGCCAcc                                                             >  1:899513/1‑140 (MQ=255)                                                        gTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCa                                                            >  2:549730/1‑140 (MQ=18)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  2:96206/1‑140 (MQ=17)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  2:1096579/1‑140 (MQ=18)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  2:1070241/1‑140 (MQ=17)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  2:1003073/1‑140 (MQ=17)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  1:926263/1‑140 (MQ=17)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  1:650285/1‑140 (MQ=18)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  1:295696/1‑140 (MQ=17)                                                         tctcAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAg                                                           >  1:1467940/1‑140 (MQ=18)                                                            cAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGa                                                        <  2:1231358/140‑1 (MQ=14)                                                             aaaTTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAg                                                       <  2:1096185/140‑1 (MQ=11)                                                             aaaTTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAg                                                       >  2:1071384/1‑140 (MQ=11)                                                             aaaTTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAg                                                       >  1:1107000/1‑140 (MQ=11)                                                                                      agcggtGGATCAGACCTACGAAACTCAACGCCGGGAAATCGAAAGTGCAGGCTTTAGAATCAAGCCACAGCACGTGATCGAAGAACACATTAGCGGCTCAGTAGCAACCAGTGAACGTCCGGGTATTAAACGgttggttg                              <  2:351376/135‑1 (MQ=1)                                                                                                                  cgacGGGAAATCGAAAGTGCAGGCTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAGCGTCCTGGTTTTAACCGGTTGCTTGCTCGCCTGAAATGTGGTGATCAattgat  >  2:301090/4‑140 (MQ=1)                                                                                                                                         |                                                                                                                     TATTTTCTGAGATGCATTATGATATGAACACCAATTTCGTATAGAGTCTCACTATGTCTCAAATTTTTGCTTACTGTCGGATATCAACGCTGGATCAGACCACCGAAAATCAACGCCGGGAAATCGAAAGTGCAGGTTTTAAAATCAAACCTCAGCAAATAATCGAAGAACACATTAGCGGCTCAGCAGCAACCAGTGAGCGTCCTGGTTTTAACCGGTTGCTTGCTCGCCTGAAATGTGGTGATCAATTGAT  >  NC_000913/1633569‑1633821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑