Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,661,914	T→C	G453G (GGT→GGC)	ynfF →	putative selenate reductase YnfF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,661,914	0	T	C	100.0%	261.5 / NA	72	G453G (GGT→GGC)	ynfF	putative selenate reductase YnfF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/33);  total (39/33)

GGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGA  >  NC_000913/1661777‑1662047                                                                                                                                          |                                                                                                                                      ggCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGgcgt                                                                                                                                     >  1:630028/1‑140 (MQ=255) ggCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGgcgt                                                                                                                                     >  1:1544003/1‑140 (MQ=255)       cgcgAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTgg                                                                                                                               >  1:810864/1‑140 (MQ=255)       cgcgAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTgg                                                                                                                               >  1:1141534/1‑140 (MQ=255)                aGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGgaaaagaaaa                                                                                                                      >  1:1330889/1‑140 (MQ=255)                aGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGgaaaagaaaa                                                                                                                      >  1:1356177/1‑140 (MQ=255)                  cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAAc                                                                                                                    >  2:1357337/1‑140 (MQ=255)                  cTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAAc                                                                                                                    >  2:416865/1‑140 (MQ=255)                     ggATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTgg                                                                                                                 <  1:132232/140‑1 (MQ=255)                        tCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATg                                                                                                              >  2:497027/1‑140 (MQ=255)                        tCTGGGGGAAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGGGGAAAAGAAAAACTGGATg                                                                                                              >  2:956934/1‑140 (MQ=255)                         cTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACAGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGt                                                                                                             >  1:1510576/1‑140 (MQ=255)                            ggggTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTccc                                                                                                          >  1:366884/1‑140 (MQ=255)                               gTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCAt                                                                                                       <  2:1107676/140‑1 (MQ=255)                                 caaaTGGTACTAGATGCTCGAGAAACCTGTTCAAACGCAGATTTCCGTCTTTACATGGACAGCCGGCATAGGCCACGGTACGGAAATGACCGCGTCCCGTGATGGCGTTCGTAGAAAAGAAAATCTGGATGTcccttaaa                                                                                                     <  2:864402/138‑6 (MQ=255)                                 aGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCa                                                                                                     <  1:1311133/140‑1 (MQ=255)                                 aGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCa                                                                                                     >  1:315263/1‑140 (MQ=255)                                   aaTGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAg                                                                                                   >  2:257049/1‑140 (MQ=255)                                        ttCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGttttt                                                                                              >  2:576986/1‑140 (MQ=255)                                         tCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                             <  2:336218/140‑1 (MQ=255)                                          cccGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTAt                                                                                            <  2:921124/140‑1 (MQ=255)                                          cccGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATAGCGTTCGTGGAAAAGAAGAACTGGATGTCCCCATCAAGTTTTTAt                                                                                            >  2:444143/1‑140 (MQ=255)                                             gATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGt                                                                                         >  2:527750/1‑140 (MQ=255)                                               tGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGc                                                                                       >  1:935740/1‑140 (MQ=255)                                                  tCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTAc                                                                                    <  2:148909/140‑1 (MQ=255)                                                  tCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTAc                                                                                    <  1:1244918/140‑1 (MQ=255)                                                   cGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACg                                                                                   >  1:790457/1‑140 (MQ=255)                                                   cGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACg                                                                                   <  1:1357337/140‑1 (MQ=255)                                                        aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGt                                                                              <  2:317007/140‑1 (MQ=255)                                                        aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGt                                                                              <  1:257049/140‑1 (MQ=255)                                                        aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGt                                                                              <  1:721471/140‑1 (MQ=255)                                                        aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGt                                                                              <  2:1050411/140‑1 (MQ=255)                                                        aTCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGt                                                                              <  1:91172/140‑1 (MQ=255)                                                         tCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                             <  2:1000395/124‑1 (MQ=255)                                                         tCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTa                                                                                             >  1:1000395/1‑124 (MQ=255)                                                           cTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAac                                                                           >  2:552416/1‑140 (MQ=255)                                                            tGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAaca                                                                          >  2:953319/1‑140 (MQ=255)                                                                aaaaCGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACAtt                                                                      <  1:467588/140‑1 (MQ=255)                                                                     gCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatca                                                                 >  1:495585/1‑140 (MQ=255)                                                                      cAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaa                                                                >  2:317821/1‑140 (MQ=255)                                                                         aTTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGatcaatca                                                             <  2:1405308/140‑1 (MQ=255)                                                                               gTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATgg                                                       <  1:416865/140‑1 (MQ=255)                                                                               gTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATgg                                                       <  2:366884/140‑1 (MQ=255)                                                                                tCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGc                                                      <  1:956934/140‑1 (MQ=255)                                                                                   ttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGAc                                                   <  1:576986/140‑1 (MQ=255)                                                                                   ttACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGAc                                                   <  2:188199/140‑1 (MQ=255)                                                                                     aCATGGACAGTTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACat                                                 <  2:1544003/140‑1 (MQ=255)                                                                                     aCATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACat                                                 <  2:630028/140‑1 (MQ=255)                                                                                          gACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACatcaatc                                            <  2:790457/140‑1 (MQ=255)                                                                                              gATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCacac                                        >  1:328550/1‑140 (MQ=255)                                                                                               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGCTGGCGTTCGTGGAAAAGAAACACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                       >  1:84156/1‑140 (MQ=255)                                                                                               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                       >  1:1115186/1‑140 (MQ=255)                                                                                               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                       >  2:325523/1‑140 (MQ=255)                                                                                               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGATCGAGGAAAAGAAAAACTGGATGGCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                       >  2:126851/1‑140 (MQ=255)                                                                                               aTGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATAGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACAcc                                       >  1:914634/1‑140 (MQ=255)                                                                                                 gCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCa                                     >  1:1055922/1‑140 (MQ=255)                                                                                                    aTCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGa                                  <  2:1356177/140‑1 (MQ=255)                                                                                                     tCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAg                                 <  2:315263/140‑1 (MQ=255)                                                                                                     tCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAg                                 >  1:1394523/1‑140 (MQ=255)                                                                                                     tCGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAg                                 <  1:552416/140‑1 (MQ=255)                                                                                                      cGACCATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAgg                                <  2:1330889/140‑1 (MQ=255)                                                                                                         ccATGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGc                             <  2:810864/140‑1 (MQ=255)                                                                                                            tGGTACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTc                          >  2:1451487/1‑140 (MQ=255)                                                                                                               tACGGAAATGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAgg                       >  1:623606/1‑140 (MQ=255)                                                                                                                      aTGACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAg                >  1:192486/1‑140 (MQ=255)                                                                                                                        gACCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCa              >  2:101281/1‑140 (MQ=255)                                                                                                                         aCCGCGACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCaa             >  2:1280741/1‑140 (MQ=255)                                                                                                                            gcgACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTg          <  2:1141534/140‑1 (MQ=255)                                                                                                                            gcgACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTg          <  1:527750/140‑1 (MQ=255)                                                                                                                              gACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCg        >  1:998770/1‑140 (MQ=255)                                                                                                                              gACCCGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCg        >  1:732890/1‑140 (MQ=255)                                                                                                                                cccGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgag      >  1:1412/1‑140 (MQ=255)                                                                                                                                cccGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgag      <  1:347133/140‑1 (MQ=255)                                                                                                                                 ccGTGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCgaga     <  2:495585/140‑1 (MQ=255)                                                                                                                                    tGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAgatga  >  2:922856/1‑140 (MQ=255)                                                                                                                                    tGATGGCGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATAAGGTGCTTCAGGATGACAGCAAGTGCGAgatga  >  2:774594/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GGCGTACGCGAAGGTAGCTGGGATCTGGGGGTAGAATGGTTCCCGATGCTCGAGAATCCTGTTAAAACGCAGATTTCCGTCTTTACATGGACAGATGCCATCGACCATGGTACGGAAATGACCGCGACCCGTGATGGTGTTCGTGGAAAAGAAAAACTGGATGTCCCCATCAAGTTTTTATGGTGCTACGCCAGTAACACATTGATCAATCAACATGGCGACATCAATCACACCCATGAGGTGCTTCAGGATGACAGCAAGTGCGAGATGA  >  NC_000913/1661777‑1662047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑