Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,674,092	G→A	P60P (CCG→CCA)	tqsA →	autoinducer 2 exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,674,092	0	G	A	100.0%	324.2 / NA	95	P60P (CCG→CCA)	tqsA	autoinducer 2 exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (54/41);  total (54/41)

ATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGC  >  NC_000913/1673955‑1674225                                                                                                                                          |                                                                                                                                      aTTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCcacg                                                                                                                                     <  1:1124295/140‑1 (MQ=255)         gggAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTgg                                                                                                                             >  2:187654/1‑140 (MQ=255)         gggAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGCTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTTCGCTGGTGTGTGCCACGTGTACTgg                                                                                                                             >  2:1391445/1‑140 (MQ=255)          ggAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGc                                                                                                                            >  1:1199839/1‑140 (MQ=255)          ggAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGc                                                                                                                            >  1:1254991/1‑140 (MQ=255)            aaTGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCgg                                                                                                                          >  1:1281442/1‑140 (MQ=255)                cTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTc                                                                                                                      >  1:79077/1‑140 (MQ=255)                cTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTc                                                                                                                      >  1:1423513/1‑140 (MQ=255)                 tGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCg                                                                                                                     >  2:1522236/1‑140 (MQ=255)                  ggTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGa                                                                                                                    >  1:222448/1‑140 (MQ=255)                   gTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGAt                                                                                                                   >  2:490187/1‑140 (MQ=255)                   gTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGAt                                                                                                                   >  1:422249/1‑140 (MQ=255)                    tCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGCtt                                                                                                                  >  2:1418324/1‑140 (MQ=255)                    tCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGGCGGtt                                                                                                                  >  2:226547/1‑140 (MQ=255)                        tattCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTtga                                                                                                              <  2:1510583/140‑1 (MQ=255)                         attCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTtgat                                                                                                             >  2:1273949/1‑140 (MQ=255)                           tctcTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTtgatga                                                                                                           <  1:1158857/140‑1 (MQ=255)                            ctctGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGAc                                                                                                          <  2:1199839/140‑1 (MQ=255)                               tGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACcat                                                                                                       >  1:421886/1‑140 (MQ=255)                               tGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGGGTCGATTTTTATGAccct                                                                                                       >  2:1518617/1‑138 (MQ=255)                               ggCGGTGTCCGCTTTGCCGCCGAGTTCATCGTGCCGTTTATTCTCGCGTTAGTGCTTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTGCGAGCGCGTGTGCTACGTGTACTGGCGGTGTCGATTTTGATGACcat                                                                                                       <  2:1205281/139‑1 (MQ=255)                                 cGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACcatca                                                                                                     >  1:1453351/1‑140 (MQ=255)                                 cGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACcatca                                                                                                     >  1:297654/1‑140 (MQ=255)                                    tATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCg                                                                                                  >  2:1121593/1‑140 (MQ=255)                                      tCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTg                                                                                                <  2:289367/140‑1 (MQ=255)                                      tCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGGCGATTTTGATGACCATCATCGTg                                                                                                >  1:159097/1‑140 (MQ=255)                                         gTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGAGTTGGCTTTTATGACCCTCATCgcggtg                                                                                             >  1:533327/1‑135 (MQ=255)                                            ttGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCg                                                                                          <  2:1423513/140‑1 (MQ=255)                                             tGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGa                                                                                         <  2:1235752/140‑1 (MQ=255)                                              gccgccGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTTATGACCATCATCGTGATGGCggt                                                                                        >  2:989287/1‑138 (MQ=255)                                              gccgccGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGCGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGGCGGTTTTCATGACCACCATCGCGATGGCgct                                                                                        >  2:887653/1‑138 (MQ=255)                                              gccgccGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCCGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGAt                                                                                        >  1:302259/1‑140 (MQ=255)                                               ccgccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATg                                                                                       <  2:635480/140‑1 (MQ=255)                                               ccgccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATg                                                                                       <  1:1522236/140‑1 (MQ=255)                                                 gccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgt                                                                                     <  1:187654/140‑1 (MQ=255)                                                  ccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgtg                                                                                    <  1:207520/140‑1 (MQ=255)                                                  ccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgtg                                                                                    <  1:991908/140‑1 (MQ=255)                                                  ccgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgtg                                                                                    <  2:422249/140‑1 (MQ=255)                                                   cgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgtgt                                                                                   <  1:266219/140‑1 (MQ=255)                                                   cgAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGgtgt                                                                                   <  1:430569/140‑1 (MQ=255)                                                            cGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGCTTTTGGTGACCATCACCTGGATGGCGGTGGTGTTGCTAtttg                                                                          >  2:1231415/1‑138 (MQ=255)                                                            cGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAg                                                                          <  1:42666/140‑1 (MQ=255)                                                            cGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAg                                                                          <  1:490187/140‑1 (MQ=255)                                                            cGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAg                                                                          <  1:1201068/140‑1 (MQ=255)                                                                ccGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGCGACGATTTTGATGAACATCATCGTGATGGCGAAGGTGTTGCTATTTGCttt                                                                      >  1:611176/1‑139 (MQ=255)                                                                 cGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTTAAGACCCTCACCGGGATTGCGATGGGGTTTCCATTAGCTTAt                                                                     >  1:1059619/1‑140 (MQ=255)                                                                 cGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCTTAt                                                                     >  1:13655/1‑140 (MQ=255)                                                                   tttATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCt                                                                   <  1:1518617/140‑1 (MQ=255)                                                                   tttATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCt                                                                   >  1:455022/1‑140 (MQ=255)                                                                    ttATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGGCGGTTTTTTAGAACAACATCGTGATGGCGATGGTGTTGCTATTTGCTTATCtt                                                                  >  2:1045321/1‑139 (MQ=255)                                                                        tctcGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCCTCATCGTGATGGCGATGGGGTTGCTATTAGCTTATCTggggt                                                              >  2:1296330/1‑138 (MQ=255)                                                                           cGCATTATTTATTGCTGTTATTCTTATCCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCcg                                                           <  2:1372799/140‑1 (MQ=255)                                                                           cGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCcg                                                           <  2:269049/140‑1 (MQ=255)                                                                           cGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCcg                                                           <  1:498329/140‑1 (MQ=255)                                                                            gCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCcgc                                                          >  1:1045653/1‑140 (MQ=255)                                                                                      ttGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAg                                                <  2:1292667/140‑1 (MQ=255)                                                                                        gCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGGGTCGGTTTTGATGACCATCATCGTGGTGGCGGTGGTGGTGCTATTAGCTTTTCTTGGTTCCGCGCTCAACGAggt                                              >  2:1532414/1‑138 (MQ=255)                                                                                            ttATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGGTGAcg                                          >  2:1326041/1‑140 (MQ=255)                                                                                            ttATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCCTATCTGGGTTCCGCGCTCAACGAGGTGAcc                                          >  2:89460/1‑139 (MQ=255)                                                                                             tATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGAcgc                                         <  2:246626/140‑1 (MQ=255)                                                                                              aTTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATTATCGTGATGGCGATGGTGGTGCTATTAGCTTTTCTTGGGTCCGCGCTCAACGGGCTGAcgcg                                        >  2:1221005/1‑140 (MQ=255)                                                                                                 cTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGAc                                     <  1:378472/140‑1 (MQ=255)                                                                                                 cTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGAc                                     <  1:636025/140‑1 (MQ=255)                                                                                                   tAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGt                                   <  1:416830/140‑1 (MQ=255)                                                                                                      cccGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGAGGGGGGTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGGTGACGCGGACGTTAc                                >  2:106394/1‑140 (MQ=255)                                                                                                        cGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCg                              <  1:1121593/140‑1 (MQ=255)                                                                                                         gCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGGTTTTGATGACCATCATCGTGGTGGCGATGGGGTTGCTATTTGCTTATCTGGGTTCCGCGCTCAAAGAGGTGACGCGGGCGTTAcccc                             >  2:406902/1‑138 (MQ=255)                                                                                                         gCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGc                             >  2:318246/1‑140 (MQ=255)                                                                                                         gCTGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGGTGCTATTAGCTTATCTGGGGTCCGCGCTCAACGAGGTGACGCGGGCGTTACCGc                             >  2:1353316/1‑140 (MQ=255)                                                                                                          cTGGTGTAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTTGGTTCCCCGCTCAACGAGTTGACGCGGACGTTACCGCa                            >  2:860389/1‑140 (MQ=255)                                                                                                           tGGTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCaa                           <  2:1488050/140‑1 (MQ=255)                                                                                                            ggTGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTTATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAat                          >  1:803594/1‑140 (MQ=255)                                                                                                              tGCAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGGGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGGCGTTACCGCAatat                        >  2:117197/1‑140 (MQ=255)                                                                                                                cAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCg                      <  2:421886/140‑1 (MQ=255)                                                                                                                cAACACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCg                      <  2:1254991/140‑1 (MQ=255)                                                                                                                 aaCACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGc                     <  2:1297538/140‑1 (MQ=255)                                                                                                                 aaCACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGc                     <  2:936998/140‑1 (MQ=255)                                                                                                                 aaCACATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGc                     <  1:473215/140‑1 (MQ=255)                                                                                                                  acacATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCa                    <  2:1045653/140‑1 (MQ=255)                                                                                                                  acacATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCa                    <  1:280560/140‑1 (MQ=255)                                                                                                                  acacATGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCa                    <  1:1375154/140‑1 (MQ=255)                                                                                                                   cacaTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCaa                   <  2:222448/140‑1 (MQ=255)                                                                                                                   cacaTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCaa                   <  2:302259/140‑1 (MQ=255)                                                                                                                    acaTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAc                  <  1:169656/140‑1 (MQ=255)                                                                                                                    acaTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAc                  <  1:662325/140‑1 (MQ=255)                                                                                                                     caTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTTATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGTGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAct                 >  1:1177517/1‑140 (MQ=255)                                                                                                                     caTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAct                 <  2:455022/140‑1 (MQ=255)                                                                                                                     caTGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGCGTCGATTTTTATGACCCTCATCCTGCTGGCGATGGGGGTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAAct                 >  2:1192298/1‑140 (MQ=255)                                                                                                                       tGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTTATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActct               >  2:1222112/1‑140 (MQ=255)                                                                                                                       tGGTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAActct               >  1:67715/1‑140 (MQ=255)                                                                                                                        ggTCCGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCta              >  1:380810/1‑140 (MQ=255)                                                                                                                          tCCGCTGGCGTGTGCCACGTGTACTGGTGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTTGCTTATCTGGGTTCCGCGCTCAACGAGTTTACGCGGACGTTACCGCAATATCGCAACTCtatt            >  2:1548201/1‑140 (MQ=255)                                                                                                                          tCCGCTGGCGCGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGGTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatt            >  2:1334183/1‑140 (MQ=255)                                                                                                                           ccGCTGGCGTGTGCCACGTGTACTGGCGGTGTCGGTTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCtatta           >  1:18858/1‑140 (MQ=255)                                                                                                                              cTGGCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGa        >  1:153819/1‑140 (MQ=255)                                                                                                                                ggCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGGTGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcg      >  2:1047040/1‑140 (MQ=255)                                                                                                                                 gCGTGTGCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcgc     <  1:1296330/140‑1 (MQ=255)                                                                                                                                    tgtgCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGGTGGGGTTGCTATTAGCTTATCTGGGGTCCGCGCTCAACGAGTTGACGCGGGCGTTACCGCCATATCGCAACTCTATTATGAcgccgc  >  2:840144/1‑140 (MQ=255)                                                                                                                                    tgtgCCACGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGAcgccgc  >  1:714881/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ATTATGTTGGGAATGCTGGTCATTATTCTCTGCGGTATCCGTTTTGCCGCCGAGATCATCGTGCCGTTTATTCTCGCATTATTTATTGCTGTTATTCTTAACCCGCTGGTGCAACACATGGTCCGCTGGCGTGTGCCGCGTGTACTGGCGGTGTCGATTTTGATGACCATCATCGTGATGGCGATGGTGTTGCTATTAGCTTATCTGGGTTCCGCGCTCAACGAGTTGACGCGGACGTTACCGCAATATCGCAACTCTATTATGACGCCGC  >  NC_000913/1673955‑1674225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑