Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,703,538	T→C	E257E (GAA→GAG)	ydgJ ←	putative oxidoreductase YdgJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,703,538	0	T	C	100.0%	268.2 / NA	72	E257E (GAA→GAG)	ydgJ	putative oxidoreductase YdgJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/36);  total (36/36)

TAGTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGAC  >  NC_000913/1703401‑1703661                                                                                                                                           |                                                                                                                            tAGTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGctc                                                                                                                           <  1:1213182/140‑1 (MQ=255) tAGTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGctc                                                                                                                           <  2:517614/140‑1 (MQ=255)    tAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGctcttc                                                                                                                        >  1:1184578/1‑140 (MQ=255)      aGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATCTCGTATGCCGTCTTCTGCTTG‑AAAAAAAACTCGCCATTTTTCAGACGCTCTTCCt                                                                                                                      >  1:1098596/1‑140 (MQ=255)       gCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCtg                                                                                                                     >  2:10841/1‑140 (MQ=255)         cGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCtgtg                                                                                                                   >  2:1447371/1‑140 (MQ=255)            aTAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGAt                                                                                                                >  2:1076364/1‑140 (MQ=255)                 tCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAgg                                                                                                           <  2:1260191/140‑1 (MQ=255)                   ccAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGcc                                                                                                         <  2:1105039/140‑1 (MQ=255)                   ccAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGcc                                                                                                         >  2:1473759/1‑140 (MQ=255)                     aGGCACCGACAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGATGAGTACGCCATCACGCATATCGAA‑GCCCCAGTCTTCCTTCGGTAGACGCTCGCCCTTTTTCAGCCGCTCTTTCTGTGGCTCGATGCCat                                                                                                       >  2:1220666/1‑140 (MQ=255)                      caggccGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCata                                                                                                      <  1:1217812/136‑1 (MQ=255)                           cGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTca                                                                                                 >  1:29106/1‑140 (MQ=255)                           cGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTca                                                                                                 >  2:1298393/1‑140 (MQ=255)                           cGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGCGGATCGAGGCCACATTTca                                                                                                 >  2:564425/1‑140 (MQ=255)                                aCAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATaa                                                                                            <  1:541584/140‑1 (MQ=255)                                 cAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAAc                                                                                           <  2:715596/140‑1 (MQ=255)                                    cGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GGCCCACTCCTCCTTGGATAGAAGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGc                                                                                        >  1:595911/1‑140 (MQ=255)                                     gTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGcc                                                                                       >  2:1139496/1‑140 (MQ=255)                                     gTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGcc                                                                                       >  2:49971/1‑140 (MQ=255)                                      tttCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCt                                                                                      >  1:630688/1‑140 (MQ=255)                                            cGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGAt                                                                                <  1:834781/140‑1 (MQ=255)                                              acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATcc                                                                              >  2:1388769/1‑140 (MQ=255)                                              acacGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATcc                                                                              <  1:1109466/140‑1 (MQ=255)                                                ccGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCAt                                                                            <  1:763455/139‑1 (MQ=255)                                                acGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCAt                                                                            <  2:815119/140‑1 (MQ=255)                                                acGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCAt                                                                            <  1:934149/140‑1 (MQ=255)                                                acGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCAt                                                                            <  1:10841/140‑1 (MQ=255)                                                 cGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATg                                                                           <  1:273791/140‑1 (MQ=255)                                                  gtgccTCGCCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGc                                                                          <  1:934274/137‑1 (MQ=255)                                                   ttCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCa                                                                         <  1:1447371/140‑1 (MQ=255)                                                    tCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCAc                                                                        >  1:511480/1‑140 (MQ=255)                                                    tCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCAc                                                                        <  1:463672/140‑1 (MQ=255)                                                     ccTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACg                                                                       <  1:1304680/140‑1 (MQ=255)                                                          ccTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGAtata                                                                  <  2:755509/140‑1 (MQ=255)                                                              ccACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGt                                                              >  1:397395/1‑140 (MQ=255)                                                              ccACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGt                                                              >  2:508288/1‑140 (MQ=255)                                                               cACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTg                                                             >  1:1498255/1‑140 (MQ=255)                                                                     ggTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACt                                                       >  1:96442/1‑140 (MQ=255)                                                                          gTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCg                                                  <  2:1498255/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:1388769/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  2:1184578/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:1545288/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:1298393/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:49971/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:957105/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  1:127180/140‑1 (MQ=255)                                                                               ccATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGc                                             <  2:94516/140‑1 (MQ=255)                                                                                cATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGcc                                            >  2:1078583/1‑140 (MQ=255)                                                                                cATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGcc                                            >  1:458599/1‑140 (MQ=255)                                                                                cATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGcc                                            >  2:1481440/1‑140 (MQ=255)                                                                                 aTCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCa                                           >  2:1140362/1‑140 (MQ=255)                                                                                    ccGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCa                                        <  1:324508/139‑1 (MQ=255)                                                                                     cGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCAt                                       >  2:682642/1‑140 (MQ=255)                                                                                         tatCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTa                                   >  2:725608/1‑140 (MQ=255)                                                                                            cGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCg                                >  1:436355/1‑140 (MQ=255)                                                                                                 gCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTa                            >  1:1476213/1‑140 (MQ=255)                                                                                                 gCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTa                            >  2:1278335/1‑140 (MQ=255)                                                                                                   cccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTaaa                          >  2:89552/1‑140 (MQ=255)                                                                                                   cccAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTaaa                          >  1:779545/1‑140 (MQ=255)                                                                                                       gTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGa                      >  2:1524993/1‑140 (MQ=255)                                                                                                           tCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                  <  2:397395/140‑1 (MQ=255)                                                                                                           tCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                  <  1:833091/140‑1 (MQ=255)                                                                                                           tCCTGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcg                  <  1:1269997/140‑1 (MQ=255)                                                                                                              tGCGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTcgccg               <  2:729199/140‑1 (MQ=255)                                                                                                                gggTGGACGCTCGCCAGTTGTCAGACGCCCCTCCTGTGGCGCGAGGCCATATTCCACGGAACTGCCTCGGGATCCATGCACGATATACCGTGCGGACTCAGCGCCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt             <  2:1098596/139‑1 (MQ=255)                                                                                                                cGGTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCt             <  1:1078583/140‑1 (MQ=255)                                                                                                                  gTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCtgt           >  1:1343200/1‑140 (MQ=255)                                                                                                                  gTAGACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCtgt           >  1:758429/1‑140 (MQ=255)                                                                                                                     gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa        <  2:1093338/140‑1 (MQ=255)                                                                                                                     gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa        <  1:1537266/140‑1 (MQ=255)                                                                                                                     gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa        <  1:1473759/140‑1 (MQ=255)                                                                                                                     gACGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGa        >  1:739816/1‑140 (MQ=255)                                                                                                                      aCGCTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGAt       >  2:1267581/1‑140 (MQ=255)                                                                                                                         cTCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAgg    <  2:96442/140‑1 (MQ=255)                                                                                                                          tCGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGa   >  1:754043/1‑140 (MQ=255)                                                                                                                           cGCCATTTTTCAGACGCTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGAc  >  2:923734/1‑140 (MQ=255)                                                                                                                                           |                                                                                                                            TAGTAAGCCGGATAATTCCCAGGCACCGTCAACAGCGTTTCTTCGACACGTTCCTCACCTTCCACGCGGGTAAGTACGCCATCACGCATATCGTA‑GCCCCAGTCTTCCTGCGGTAGACGCTCGCCATTTTTCAGACGTTCTTCCTGTGGATCGAGGCCATATTTCACATAACTGCCTCGGGATCCATGCACGATATACCGTGCTGACTCAGCGGCTGCCAGCATGGTACCGTGTAAAATGACTCGCCGCTGTGGATAGGAC  >  NC_000913/1703401‑1703661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑