Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,257	A→T	I31N (ATC→AAC)	ydhU ←	putative cytochrome YdhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,257	0	A	T	100.0%	230.8 / NA	69	I31N (ATC→AAC)	ydhU	putative cytochrome YdhU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (37/32);  total (37/32)

TGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATAC  >  NC_000913/1750129‑1750390                                                                                                                                 |                                                                                                                                      tGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACAcc                                                                                                                            >  1:621160/1‑140 (MQ=255) tGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACAcc                                                                                                                            >  1:551554/1‑140 (MQ=255) tGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACAcc                                                                                                                            >  2:286991/1‑140 (MQ=255) tGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACAcc                                                                                                                            >  1:243871/1‑140 (MQ=255)   aCCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCgg                                                                                                                          >  1:1138450/1‑140 (MQ=255)   aCCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCgg                                                                                                                          >  2:507716/1‑140 (MQ=255)       cTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAACCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCaa                                                                                                                      >  2:406354/1‑140 (MQ=255)        tttGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCaaa                                                                                                                     >  1:429654/1‑140 (MQ=255)         ttGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCaaaa                                                                                                                    >  1:1290690/1‑140 (MQ=255)           gCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAAtt                                                                                                                  <  1:447561/140‑1 (MQ=255)               tAAAGATAAATCGTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCggg                                                                                                              >  1:16288/1‑140 (MQ=255)                 aaGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAg                                                                                                            >  1:374142/1‑140 (MQ=255)                    aTAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGa                                                                                                         >  1:1113123/1‑140 (MQ=255)                     tAAATCTTCTCCCCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGaa                                                                                                        <  2:115115/140‑1 (MQ=255)                     tAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGaa                                                                                                        <  1:614705/140‑1 (MQ=255)                     tAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGaa                                                                                                        <  2:1528971/140‑1 (MQ=255)                     tAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGaa                                                                                                        <  2:831705/140‑1 (MQ=255)                      aaaTCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAAc                                                                                                       >  1:1106374/1‑140 (MQ=255)                       aaTCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACt                                                                                                      <  1:1111922/140‑1 (MQ=255)                       aaTCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACt                                                                                                      >  2:1107791/1‑140 (MQ=255)                       aaTCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACt                                                                                                      <  1:938038/140‑1 (MQ=255)                             ctcACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGca                                                                                                >  2:1313260/1‑140 (MQ=255)                             ctcACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGca                                                                                                >  2:791740/1‑140 (MQ=255)                             ctcACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGca                                                                                                <  2:931253/140‑1 (MQ=255)                                  cATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAg                                                                                           <  1:1001663/140‑1 (MQ=255)                                   aTGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGt                                                                                          <  2:897978/140‑1 (MQ=255)                                     gATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTc                                                                                        >  2:1020716/1‑140 (MQ=255)                                          gTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTaa                                                                                   >  1:586593/1‑140 (MQ=255)                                          gTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTaa                                                                                   >  2:1086838/1‑140 (MQ=255)                                            ggCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACt                                                                                 <  2:172409/140‑1 (MQ=255)                                            ggCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACt                                                                                 <  2:551554/140‑1 (MQ=255)                                             gCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTg                                                                                >  1:1118653/1‑140 (MQ=255)                                                gATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGct                                                                             >  2:617804/1‑140 (MQ=255)                                                 aTTTCTTCACGCCACGAGCACGAAGCAAGGAATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACTCCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGctc                                                                            <  2:50285/140‑1 (MQ=255)                                                    tcttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGa                                                                         <  1:454594/140‑1 (MQ=255)                                                     cttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAACACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGaa                                                                        >  1:146298/1‑140 (MQ=255)                                                      ttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGaaa                                                                       <  2:243871/140‑1 (MQ=255)                                                      ttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGaaa                                                                       <  2:1053094/140‑1 (MQ=255)                                                      ttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGaaa                                                                       <  2:355828/140‑1 (MQ=255)                                                      ttcACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGaaa                                                                       <  2:3422/140‑1 (MQ=255)                                                        cACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACt                                                                     >  1:712373/1‑140 (MQ=255)                                                             cACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCa                                                                <  2:374142/140‑1 (MQ=255)                                                             cACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCa                                                                <  1:1107791/140‑1 (MQ=255)                                                                     cGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTg                                                        >  1:558014/1‑140 (MQ=255)                                                                        aGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGa                                                     <  1:1086838/140‑1 (MQ=255)                                                                        aGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGa                                                     <  1:286991/140‑1 (MQ=255)                                                                        aGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGa                                                     <  1:375004/140‑1 (MQ=255)                                                                        aGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATGCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGa                                                     <  2:16288/140‑1 (MQ=255)                                                                        aGCAAGGCATGCAGCCCCAGCACCAACCACACCCCAACCAGCAATACTCCGGCAATGTTCACCCACACCGACCAAAATTCGGGAGTGAACTGTGGCACATAGATCGCTAACTCGCTCTGAACTTGTTCAGCATGTTGCGa                                                     >  2:599758/1‑140 (MQ=255)                                                                         gCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGAc                                                    <  1:1410977/140‑1 (MQ=255)                                                                            aGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACggg                                                 >  1:284582/1‑140 (MQ=255)                                                                             ggCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGt                                                >  2:841440/1‑140 (MQ=255)                                                                             ggCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGt                                                >  2:1223369/1‑140 (MQ=255)                                                                               cATGCAGCCCCAGCACCAACCCCATCCCAACCAGCAATTCTCCGGCAATGTTCAACCCCACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTc                                              <  2:1372127/140‑1 (MQ=255)                                                                                aTGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCa                                             <  2:429654/140‑1 (MQ=255)                                                                                      ccccAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACAtt                                       <  2:1113123/140‑1 (MQ=255)                                                                                        ccAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACAtttt                                     >  2:1230743/1‑140 (MQ=255)                                                                                           gCACCAACCACATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCt                                  >  2:116022/1‑140 (MQ=255)                                                                                              ccaaccaCATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTGAGCATGTTGCGACGGGTTCATACATTTTCCtttt                               <  1:599758/140‑1 (MQ=255)                                                                                                aaccaCATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTa                             >  1:972417/1‑140 (MQ=255)                                                                                                 accaCATCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCATTTTaa                            >  2:1118820/1‑140 (MQ=255)                                                                                                     caTCCCAACCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCaa                        >  1:1238390/1‑140 (MQ=255)                                                                                                            aCCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGa                 >  1:525866/1‑140 (MQ=255)                                                                                                            aCCAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGCGAACTGTGGCACATAGATCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCATTTTAATCAAATGTTGa                 >  1:1277568/1‑140 (MQ=255)                                                                                                             ccAGCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGAc                >  2:1050705/1‑140 (MQ=255)                                                                                                                gCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGa             <  1:507716/140‑1 (MQ=255)                                                                                                                gCAATACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGa             <  2:146298/140‑1 (MQ=255)                                                                                                                   aTACTCCGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAAcc          <  1:617804/140‑1 (MQ=255)                                                                                                                        ccGGCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGa     >  2:850684/1‑140 (MQ=255)                                                                                                                          ggCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATa   >  1:893375/1‑140 (MQ=255)                                                                                                                           gCAATGTTCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATAc  >  2:1347101/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                      TGACCGCTTTGCTGTAAAGATAAATCTTCTCACCATGATCGGTGGCTGATTTCTTCACGCCACGAGCACGAAGCAAGGCATGCAGCCCCAGCACCAACCACATCCCAACCAGCAATACTCCGGCAATGATCAACCACACCGGCCAAAATTCGGGAGTGAACTGTGGCACATAGTTCGCTAACTGGCTCTGAAACTGTTCAGCATGTTGCGACGGGTTCATACATTTTCCTTTTTAATCAAATGTTGACCGAACCGGCGATAC  >  NC_000913/1750129‑1750390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑