Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,183	T→C	N217S (AAC→AGC)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,183	0	T	C	100.0%	251.4 / NA	67	N217S (AAC→AGC)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/35);  total (32/35)

AACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996048‑1996318                                                                                                                                         |                                                                                                                                        aaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGctttt                                                                                                                                     >  2:281689/1‑140 (MQ=255) aaCCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGctttt                                                                                                                                     >  2:896629/1‑140 (MQ=255)   ccGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCt                                                                                                                                   >  2:75361/1‑140 (MQ=255)   ccGGCTGATAGCGTGCATTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCt                                                                                                                                   >  1:1541405/1‑140 (MQ=255)    cGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCtg                                                                                                                                  >  2:517811/1‑140 (MQ=255)     ggCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCtgg                                                                                                                                 <  2:622373/140‑1 (MQ=255)     ggCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCtgg                                                                                                                                 <  1:184035/140‑1 (MQ=255)      gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:917755/1‑140 (MQ=255)              cGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGACTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTg                                                                                                                        >  2:1478477/1‑140 (MQ=255)               gTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGa                                                                                                                       <  1:1128924/140‑1 (MQ=255)               gTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGa                                                                                                                       >  1:890496/1‑140 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  2:846982/140‑1 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  2:1035079/140‑1 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  2:1486376/140‑1 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  1:278707/1‑140 (MQ=255)                tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:1126713/1‑140 (MQ=255)                 gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                     <  1:601631/140‑1 (MQ=255)                 gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                     <  2:1384764/140‑1 (MQ=255)                 gCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                     <  2:1533506/140‑1 (MQ=255)                   cTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGt                                                                                                                   <  2:1132342/140‑1 (MQ=255)                    tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  2:104169/140‑1 (MQ=255)                    tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  2:323590/140‑1 (MQ=255)                    tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  2:917755/140‑1 (MQ=255)                    tttCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTa                                                                                                                  <  2:986355/140‑1 (MQ=255)                        aGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATtct                                                                                                              <  2:156113/140‑1 (MQ=255)                          ccGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCa                                                                                                            <  1:983963/140‑1 (MQ=255)                             gTTTTTGGATTTGTCACGCCTGACAGTATAGAGATTAAATTAAGCCAGGAGCATCCGCGTAACAGGCAACCTGGGTCTTATTTGGAGCATT‑AATTTTTTTCTGCATGCTTTTCTGGGGGATATTGACCGTATTCTCagag                                                                                                         >  2:794917/1‑140 (MQ=255)                                  tGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                     <  1:731925/123‑1 (MQ=255)                                  tGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAcc                                                                                                                     >  2:731925/1‑123 (MQ=255)                                        tGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAat                                                                                              >  2:270659/1‑140 (MQ=255)                                        tGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAat                                                                                              >  2:544827/1‑140 (MQ=255)                                         ggCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatc                                                                                             >  2:382489/1‑140 (MQ=255)                                          gCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatca                                                                                            >  1:916302/1‑140 (MQ=255)                                              gCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGc                                                                                        <  1:1126713/140‑1 (MQ=255)                                               cAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCt                                                                                       <  1:1316870/140‑1 (MQ=255)                                               cAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCt                                                                                       >  2:1284543/1‑140 (MQ=255)                                                    cAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAtctc                                                                                  >  2:657033/1‑140 (MQ=255)                                                       aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                               >  1:187731/1‑140 (MQ=255)                                                       aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                               <  1:205147/140‑1 (MQ=255)                                                            agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                          >  1:461094/1‑140 (MQ=255)                                                              aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGt                                                                         >  2:909733/1‑140 (MQ=255)                                                              aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATTTCTGCTGATGtt                                                                        >  2:812275/1‑140 (MQ=255)                                                              aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                        >  1:1392915/1‑140 (MQ=255)                                                               tCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGttt                                                                       <  1:469662/140‑1 (MQ=255)                                                                     tAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCtt                                                                 <  2:1541405/140‑1 (MQ=255)                                                                      aaGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTc                                                                <  2:1028382/140‑1 (MQ=255)                                                                        gCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTccg                                                              <  1:517811/140‑1 (MQ=255)                                                                             tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         >  2:1239778/1‑140 (MQ=255)                                                                                cGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCAc                                                      <  2:1080271/140‑1 (MQ=255)                                                                                      cgTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGa                                                >  2:561729/1‑140 (MQ=255)                                                                                       gTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGaa                                               >  1:396851/1‑140 (MQ=255)                                                                                          aCAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAAttt                                            <  1:896629/140‑1 (MQ=255)                                                                                             ggCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCtt                                         >  2:904541/1‑140 (MQ=255)                                                                                                 aCCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTc                                     >  1:345449/1‑140 (MQ=255)                                                                                                    tGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgtg                                  <  1:1241614/140‑3 (MQ=255)                                                                                                    tGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTcgcg                                  <  2:11492/140‑1 (MQ=255)                                                                                                      ggTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCt                                <  1:561729/140‑1 (MQ=255)                                                                                                            aTTTGGTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGa                          >  1:35856/1‑140 (MQ=255)                                                                                                                ggTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      >  2:833830/1‑140 (MQ=255)                                                                                                                ggTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      <  1:1478477/140‑1 (MQ=255)                                                                                                                 gTGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTc                     <  2:278707/140‑1 (MQ=255)                                                                                                                  tGCATT‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCa                    >  2:815277/1‑140 (MQ=255)                                                                                                                      tt‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAtctc                >  1:606745/1‑140 (MQ=255)                                                                                                                       t‑AATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATctct               <  2:916302/140‑1 (MQ=255)                                                                                                                            ttttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCg           <  1:1284543/140‑1 (MQ=255)                                                                                                                                ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  1:544827/140‑1 (MQ=255)                                                                                                                                  cTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcatca     >  1:259976/1‑140 (MQ=255)                                                                                                                                  cTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcatca     <  1:909733/140‑1 (MQ=255)                                                                                                                                    gCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACt   >  1:1344403/1‑140 (MQ=255)                                                                                                                                     cATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTa  >  1:201222/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        AACCGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATT‑AATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996048‑1996318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑