Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,166,877	C→T	H404Y (CAC→TAC)	yegQ →	putative peptidase YegQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,166,877	0	C	T	100.0%	224.4 / NA	66	H404Y (CAC→TAC)	yegQ	putative peptidase YegQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/37);  total (29/37)

ACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAACCACGCGTA  >  NC_000913/2166740‑2167012                                                                                                                                          |                                                                                                                                        aCCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGtac                                                                                                                                       >  1:1100971/1‑140 (MQ=255) aCCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGtac                                                                                                                                       >  2:583032/1‑140 (MQ=255)  ccGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGtaca                                                                                                                                      <  2:1376657/140‑1 (MQ=255)  ccGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGtaca                                                                                                                                      <  1:1391258/140‑1 (MQ=255)                  ggTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAgg                                                                                                                      <  2:364830/140‑1 (MQ=255)                    tGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCg                                                                                                                    <  1:1076695/140‑1 (MQ=255)                       gTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAg                                                                                                                 <  2:425075/140‑1 (MQ=255)                        tttACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGc                                                                                                                <  2:267473/140‑1 (MQ=255)                          tACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTa                                                                                                              >  1:1102609/1‑140 (MQ=255)                           aCCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTAt                                                                                                             <  1:531206/140‑1 (MQ=255)                                  aGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATa                                                                                                      >  2:526473/1‑140 (MQ=255)                                    cgcAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGc                                                                                                    >  1:1059163/1‑140 (MQ=255)                                    cgcAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGc                                                                                                    >  2:804472/1‑140 (MQ=255)                                         gggggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAg                                                                                               >  2:1031316/1‑140 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTGATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:714823/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTTCCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  1:1474236/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  1:1508980/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:1022217/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:6056/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:863581/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:1085255/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:1523746/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  1:563047/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  1:1053288/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  2:246890/140‑1 (MQ=255)                                           gggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGc                                                                                             <  1:708870/140‑1 (MQ=255)                                            ggACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCg                                                                                            <  2:953655/140‑1 (MQ=255)                                             gACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa                                                                                           <  2:736680/140‑1 (MQ=255)                                             gACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGa                                                                                           <  2:925289/140‑1 (MQ=255)                                                cTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATgg                                                                                        <  1:1350007/140‑1 (MQ=255)                                                cTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATgg                                                                                        <  1:324098/140‑1 (MQ=255)                                                 tCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGt                                                                                       >  1:1180432/1‑140 (MQ=255)                                                   gcggcgGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACACCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTta                                                                                     <  1:1364841/140‑1 (MQ=255)                                                     ggcggTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTtata                                                                                   <  2:774563/140‑1 (MQ=255)                                                          tAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAAAATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACtgtg                                                                              >  2:963871/1‑140 (MQ=255)                                                                tGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTc                                                                        <  2:252104/140‑1 (MQ=255)                                                                 gAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTcc                                                                       <  2:265428/140‑1 (MQ=255)                                                                 gAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTcc                                                                       <  1:1386449/140‑1 (MQ=255)                                                                   aaaaTAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCg                                                                     >  2:1268355/1‑140 (MQ=255)                                                                    aaataaatTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCgg                                                                    <  1:756160/140‑1 (MQ=255)                                                                      ataaatTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTc                                                                  <  2:1102609/140‑1 (MQ=255)                                                                           ttCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCa                                                             <  1:1424834/140‑1 (MQ=255)                                                                            tctcCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAg                                                            >  1:437259/1‑140 (MQ=255)                                                                                cGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATc                                                        >  1:487887/1‑140 (MQ=255)                                                                                  ttGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCtt                                                      <  1:526473/140‑1 (MQ=255)                                                                                     gCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAGCGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAg                                                   <  1:1425578/140‑1 (MQ=255)                                                                                     gCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAg                                                   <  2:1215908/140‑1 (MQ=255)                                                                                     gCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAg                                                   >  1:275139/1‑140 (MQ=255)                                                                                          aGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCaa                                              >  2:1444654/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  2:364080/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  1:257758/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  1:448467/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  1:138852/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  2:153914/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  1:1334325/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  2:1297816/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  2:1244941/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  2:1046061/1‑140 (MQ=255)                                                                                                      atgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCgctgct                                  >  1:906652/1‑140 (MQ=255)                                                                                                       tgaCGCCGCAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGctgctg                                 >  1:827383/1‑140 (MQ=255)                                                                                                           gccgcAAGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGc                             >  1:122691/1‑140 (MQ=255)                                                                                                                aaGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAAt                        >  2:204551/1‑140 (MQ=255)                                                                                                                aaGGCAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAAt                        >  2:1424627/1‑140 (MQ=255)                                                                                                                    cAACATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTtct                    >  2:636459/1‑140 (MQ=255)                                                                                                                       cATTAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCg                 >  1:602151/1‑140 (MQ=255)                                                                                                                          tAATTTTACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCgggg              <  2:1180432/140‑1 (MQ=255)                                                                                                                              tttACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAAc          <  2:448467/140‑1 (MQ=255)                                                                                                                              tttACCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAAc          <  2:166307/140‑1 (MQ=255)                                                                                                                                 aCCCTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAACCAc       >  1:453997/1‑140 (MQ=255)                                                                                                                                  cccTTGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCTGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAACCAcg      >  2:387625/1‑140 (MQ=255)                                                                                                                                      tGAGTACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAACCACGCGTa  <  1:583032/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ACCGCCAGCAGTTTGTTGGTGAGTTTACCGGTGAGCGCAAGGGGGACCTCGCGGCGGTAGCGGTGAAAAATAAATTCTCCGTTGGCGACAGCCTTGAGCTGATGACGCCGCAAGGCAACATTAATTTTACCCTTGAGCACATGGAAAACGCCAAAGGCGAAGCTATGCCGATAGCACCAGGCGATGGTTATACTGTGTGGCTCCCGGTCCCGCAGGATCTTGAGCTCAATTACGCGCTGCTGATGCGTAATTTCTCCGGGGAAACCACGCGTA  >  NC_000913/2166740‑2167012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑