Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,556,769	(T)9→8	coding (116/144 nt)	ytiD →	protein YtiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,556,761	0	T	.	100.0%	395.8 / NA	89	coding (108/144 nt)	ytiD	protein YtiD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (53/36);  total (53/36)

TACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATG  >  NC_000913/4556628‑4556895                                                                                                                                      |                                                                                                                                       tACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttt                                                                                                                                  >  1:32852/1‑140 (MQ=255)    aaaCTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGttttttttga                                                                                                                               >  2:815432/1‑138 (MQ=255)         aaTTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTtg                                                                                                                         >  1:1421691/1‑140 (MQ=255)                    ataataCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTAt                                                                                                              >  1:1449849/1‑140 (MQ=255)                        taCATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCaaa                                                                                                          >  2:322828/1‑140 (MQ=255)                            tGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCAt                                                                                                      >  2:1344126/1‑140 (MQ=255)                             ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  1:634452/1‑140 (MQ=255)                             ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  1:595039/1‑140 (MQ=255)                             ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  2:195427/1‑140 (MQ=255)                             ggCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATg                                                                                                     >  2:876766/1‑140 (MQ=255)                              gCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGa                                                                                                    >  2:1164547/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  1:397064/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  2:156701/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  1:1155137/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttt                                                                                                >  1:1391178/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCACGACttt                                                                                                >  2:886748/1‑140 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTCTGATGATTTTTAt                                                                                                              >  1:1204555/1‑126 (MQ=255)                                  aTTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTCTGATGATTTTTAt                                                                                                              <  2:1204555/126‑1 (MQ=255)                                    tATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACttttt                                                                                              >  2:1117865/1‑140 (MQ=255)                                        cGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCt                                                                                          >  2:102906/1‑140 (MQ=255)                                          gAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGa                                                                                        <  1:1117865/140‑1 (MQ=255)                                           aaataaatAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaa                                                                                       >  1:265140/1‑140 (MQ=255)                                            aataaataATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa                                                                                      >  1:821531/1‑140 (MQ=255)                                            aataaataATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaa                                                                                      <  1:856989/140‑1 (MQ=255)                                             ataaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGaaaa                                                                                     <  1:876766/140‑1 (MQ=255)                                             ataaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTAaaaa                                                                                     >  2:1014453/1‑140 (MQ=255)                                               aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt                                                                                   <  2:594211/140‑1 (MQ=255)                                               aaataaTTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGt                                                                                   <  2:136973/140‑1 (MQ=255)                                                aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                  >  1:1232315/1‑140 (MQ=255)                                                aataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTc                                                                                  >  1:1187292/1‑140 (MQ=255)                                                 ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCa                                                                                 <  2:1099946/140‑1 (MQ=255)                                                 ataatTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCa                                                                                 >  1:822613/1‑140 (MQ=255)                                                     tttCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAg                                                                             <  1:815432/140‑1 (MQ=255)                                                       tCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat                                                                           >  1:64821/1‑140 (MQ=255)                                                       tCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGat                                                                           >  1:1501171/1‑140 (MQ=255)                                                            ccGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCa                                                                      >  1:952733/1‑140 (MQ=255)                                                              ggAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGGATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATa                                                                    <  1:95177/140‑1 (MQ=255)                                                                 aTTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGc                                                                 >  2:692273/1‑140 (MQ=255)                                                                  ttAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCa                                                                >  2:490786/1‑140 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:1453159/140‑1 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            >  1:1245481/1‑140 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  2:1391178/140‑1 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  2:265140/140‑1 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  2:32852/140‑1 (MQ=255)                                                                      gTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCACGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTa                                                            <  1:886748/140‑1 (MQ=255)                                                                       tAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTaa                                                           >  1:1422809/1‑140 (MQ=255)                                                                       tAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTaa                                                           >  2:1510522/1‑140 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        >  2:35660/1‑140 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        >  1:561620/1‑140 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        <  2:1155137/140‑1 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        >  1:1169085/1‑140 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        >  1:1413348/1‑140 (MQ=255)                                                                          cagcGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGt                                                        >  2:88155/1‑140 (MQ=255)                                                                             cGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGtttt                                                     >  1:322024/1‑140 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   <  2:1501171/140‑1 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   >  1:1053635/1‑140 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   >  2:516881/1‑140 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   >  2:1529602/1‑140 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   >  1:1389074/1‑140 (MQ=255)                                                                               gTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTtat                                                   >  1:578539/1‑140 (MQ=255)                                                                                 gACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATAc                                                 <  1:322828/140‑1 (MQ=255)                                                                                  aCAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACt                                                <  2:1422809/140‑1 (MQ=255)                                                                                      gTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGc                                            >  1:793110/1‑140 (MQ=255)                                                                                         ttttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGa                                         <  2:595039/140‑1 (MQ=255)                                                                                           ttCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACa                                       <  2:793110/140‑1 (MQ=255)                                                                                            tCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGACAAGACAGTAGATATTCATAGACAAGAAAGGTTTTATACTTTGCTGACAg                                      >  2:575408/1‑140 (MQ=255)                                                                                              aTCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGa                                    >  2:856743/1‑140 (MQ=255)                                                                                               tCTACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGAt                                   <  2:1000005/140‑1 (MQ=255)                                                                                                 tACGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTc                                 >  2:1147840/1‑140 (MQ=255)                                                                                                   cGTAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAg                               >  2:36520/1‑140 (MQ=255)                                                                                                     tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:195427/140‑1 (MQ=255)                                                                                                     tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:1164547/140‑1 (MQ=255)                                                                                                     tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  2:1413348/140‑1 (MQ=255)                                                                                                     tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:692273/140‑1 (MQ=255)                                                                                                     tAACTATTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGc                             <  1:412278/140‑1 (MQ=255)                                                                                                          aTTCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtc                        <  1:1529602/140‑1 (MQ=255)                                                                                                           ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                       <  1:367271/140‑1 (MQ=255)                                                                                                           ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                       <  2:1232315/140‑1 (MQ=255)                                                                                                           ttCGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTAAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGtct                       <  1:1014453/140‑1 (MQ=255)                                                                                                             cGGAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCa                     >  1:250512/1‑140 (MQ=255)                                                                                                               gAATATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGa                   <  2:64821/140‑1 (MQ=255)                                                                                                                aaTATTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGAc                  <  1:1510522/140‑1 (MQ=255)                                                                                                                 atatTCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACt                 <  2:634452/140‑1 (MQ=255)                                                                                                                     tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa             >  1:734593/1‑140 (MQ=255)                                                                                                                     tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa             >  2:1577842/1‑140 (MQ=255)                                                                                                                     tCAGAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACa             >  2:264831/1‑140 (MQ=255)                                                                                                                        gAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATgg          <  2:822613/140‑1 (MQ=255)                                                                                                                        gAATATACTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATgg          <  1:156701/140‑1 (MQ=255)                                                                                                                          atatACTAGCGG‑TTTTTTTTGAGTTTGATGGTTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGAt        >  1:1516051/1‑140 (MQ=255)                                                                                                                              aCTAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTaa    >  2:906890/1‑140 (MQ=255)                                                                                                                                tAGCGG‑TTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATg  <  2:1053635/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                       TACAAACTAATTGTTTCAAATAATACATGGCTGATTATGCGGAAATAAATAATTTCCCACCGGAATTAAGTAGCAGCGGTGACAAGTATTTTCATCTACGTAACTATTCGGAATATTCAGAATATACTAGCGGTTTTTTTTTGAGTTTGATGATTTTTATCAAATCATGACTTTTTTCCTGAAAAGTCAGTAGATATTCATAGGCAAGTAAGGTTTTATACTTTGCTGACAGGATTCAGGCCTGTCTCAGACTGACATGGATGTAATG  >  NC_000913/4556628‑4556895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑