Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	932,340	(A)8→7	intergenic (‑290/‑255)	trxB ← / → lrp	thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	932,333	0	A	.	100.0%	378.5 / NA	85	intergenic (‑283/‑262)	trxB/lrp	thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (41/44);  total (41/44)

TGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCT  >  NC_000913/932194‑932465                                                                                                                                            |                                                                                                                                     tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaaga                                                                                                                                      >  1:584770/1‑140 (MQ=255) tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaaga                                                                                                                                      >  1:566730/1‑140 (MQ=255) tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaaga                                                                                                                                      >  1:1143958/1‑140 (MQ=255) tGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGAAGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGgaaga                                                                                                                                      >  2:512556/1‑140 (MQ=255)   cTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaa                                                                                                                                    >  2:1168545/1‑140 (MQ=255)       ttAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                                <  1:501882/140‑1 (MQ=255)       ttAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa                                                                                                                                <  1:473721/140‑1 (MQ=255)         aaaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                              <  1:1341163/140‑3 (MQ=255)         aaaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca                                                                                                                              <  1:175903/140‑3 (MQ=255)           aTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaacagt                                                                                                                            <  1:1134821/140‑5 (MQ=255)           aTTTCCCGC‑TTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTa                                                                                                                          >  1:507904/1‑140 (MQ=255)             ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAt                                                                                                                         >  2:749601/1‑140 (MQ=255)             ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAt                                                                                                                         >  1:445339/1‑140 (MQ=255)                ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCt                                                                                                                      >  2:1079571/1‑140 (MQ=255)                   cTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat                                                                                                                   <  2:165822/140‑1 (MQ=255)                   cTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat                                                                                                                   <  2:1187786/140‑1 (MQ=255)                   cTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat                                                                                                                   <  2:283190/140‑1 (MQ=255)                          aGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCa                                                                                                            <  1:1289672/140‑1 (MQ=255)                           gCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCAt                                                                                                           <  2:922205/140‑1 (MQ=255)                             cGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATaa                                                                                                         <  2:1354257/140‑1 (MQ=255)                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAAc                                                                                                        >  2:1090244/1‑140 (MQ=255)                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAAc                                                                                                        <  1:594723/140‑1 (MQ=255)                              gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAAc                                                                                                        >  1:344313/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      >  2:681709/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      >  1:1023396/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      >  1:671167/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      >  1:979632/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      >  1:702840/1‑140 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      <  2:472890/140‑1 (MQ=255)                                ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACca                                                                                                      <  2:507904/140‑1 (MQ=255)                                  aaaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                                    <  2:639543/140‑1 (MQ=255)                                  aaaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatg                                                                                                    <  1:255030/140‑1 (MQ=255)                                   aaTGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatgc                                                                                                   <  2:621761/140‑1 (MQ=255)                                    atgatgAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatgca                                                                                                  <  1:1492342/140‑1 (MQ=255)                                       atgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGt                                                                                               <  2:147018/140‑1 (MQ=255)                                          aaTAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTaaa                                                                                            >  2:152431/1‑140 (MQ=255)                                          aaTAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTaaa                                                                                            >  2:207394/1‑140 (MQ=255)                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATa                                                                                          <  2:639801/140‑1 (MQ=255)                                            tAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATa                                                                                          <  1:1179982/140‑1 (MQ=255)                                             aaaCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc                                                                                         <  2:897657/140‑1 (MQ=255)                                               aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCa                                                                                       >  2:96548/1‑140 (MQ=255)                                               aCGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCa                                                                                       <  1:391539/140‑1 (MQ=255)                                                  ccccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGt                                                                                    >  2:959524/1‑140 (MQ=255)                                                   cccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGtt                                                                                   >  2:1562468/1‑140 (MQ=255)                                                    ccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGttt                                                                                  >  2:1139778/1‑140 (MQ=255)                                                     cTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa                                                                                 <  1:512556/140‑1 (MQ=255)                                                      tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTAc                                                                                <  2:788951/140‑1 (MQ=255)                                                        ttAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCg                                                                              <  1:395280/140‑1 (MQ=255)                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGc                                                                           >  1:757967/1‑140 (MQ=255)                                                           aTGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGc                                                                           >  1:713297/1‑140 (MQ=255)                                                               atatCTGGCATGTTGTACTAAAAATCGATTGTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                       <  2:941999/140‑1 (MQ=255)                                                               atatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGt                                                                       <  1:1023974/140‑1 (MQ=255)                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTg                                                                      <  1:96263/140‑1 (MQ=255)                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTg                                                                      <  2:1394356/140‑1 (MQ=255)                                                                tatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTg                                                                      <  2:445339/140‑1 (MQ=255)                                                                 atCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa                                                                     <  2:385552/140‑1 (MQ=255)                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaa                                                                    >  2:774750/1‑140 (MQ=255)                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaa                                                                    >  1:180139/1‑140 (MQ=255)                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaa                                                                    >  2:246864/1‑140 (MQ=255)                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaa                                                                    >  1:30306/1‑140 (MQ=255)                                                                  tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaa                                                                    >  1:1012464/1‑140 (MQ=255)                                                                   cTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGaaa                                                                   <  1:1070818/140‑1 (MQ=255)                                                                     ggCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATc                                                                 <  2:759146/140‑1 (MQ=255)                                                                     ggCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATc                                                                 >  2:971099/1‑140 (MQ=255)                                                                     ggCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATc                                                                 >  1:540427/1‑140 (MQ=255)                                                                      gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCt                                                                >  1:78177/1‑140 (MQ=255)                                                                          gttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                            <  2:671167/140‑1 (MQ=255)                                                                          gttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt                                                            <  2:584770/140‑1 (MQ=255)                                                                            tgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTAt                                                          >  1:1020158/1‑140 (MQ=255)                                                                             gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATg                                                         >  1:748003/1‑140 (MQ=255)                                                                             gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATg                                                         <  1:749601/140‑1 (MQ=255)                                                                               aCTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc                                                       <  1:96548/140‑1 (MQ=255)                                                                                cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCg                                                      >  2:1003497/1‑140 (MQ=255)                                                                                     aaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGAc                                                 >  1:1535285/1‑140 (MQ=255)                                                                                     aaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGAc                                                 >  2:344546/1‑140 (MQ=255)                                                                                      aTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACa                                                <  2:344313/140‑1 (MQ=255)                                                                                        cGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGa                                              >  1:1162073/1‑140 (MQ=255)                                                                                        cGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGa                                              <  2:1081744/140‑1 (MQ=255)                                                                                          aTGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACg                                            <  1:959524/140‑1 (MQ=255)                                                                                                  cTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGt                                    <  2:748003/140‑1 (MQ=255)                                                                                                           tCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGAt                           <  2:757967/140‑1 (MQ=255)                                                                                                                tGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACGGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTg                      >  2:65605/1‑140 (MQ=255)                                                                                                                tGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGGTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTg                      >  1:258962/1‑140 (MQ=255)                                                                                                                       ttGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAg               >  2:369394/1‑140 (MQ=255)                                                                                                                        tGCGCAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAgg              >  2:1359455/1‑140 (MQ=255)                                                                                                                        tGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAgg              >  2:1077327/1‑140 (MQ=255)                                                                                                                        tGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAgg              >  1:808543/1‑140 (MQ=255)                                                                                                                            aaaaCATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAc          >  1:594704/1‑140 (MQ=255)                                                                                                                            aaaaCATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCACATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAc          >  1:1025436/1‑140 (MQ=255)                                                                                                                               aCATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGt       <  1:1562468/140‑1 (MQ=255)                                                                                                                               aCATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGt       <  1:1408107/140‑1 (MQ=255)                                                                                                                                  ttCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGtctt    <  2:1535285/140‑1 (MQ=255)                                                                                                                                    cGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGtcttct  <  1:207394/140‑1 (MQ=255)                                                                                                                                    cGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGtcttct  <  1:1090244/140‑1 (MQ=255)                                                                                                                                    cGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGAAGTCGATAGCTGCCACAAGGCAACGGtcttct  <  2:1020158/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     TGCTACTTAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAACGGTCTTCT  >  NC_000913/932194‑932465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑