BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,128,145	(T)_8→7	intergenic (‑124/‑127)	glcD ← / → glcC	glycolate dehydrogenase, putative FAD‑linked subunit/DNA‑binding transcriptional dual regulator GlcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,128,138	0	T	.	100.0%	483.6 / NA	108	intergenic (‑117/‑134)	glcD/glcC	glycolate dehydrogenase, putative FAD‑linked subunit/DNA‑binding transcriptional dual regulator GlcC
Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (48/60); total (48/60)

Only 100 of 124 total aligned reads displayed.

GCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAAT  >  NC_000913/3128000‑3128273

gCTCTTCGTACATGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtt                                                                                                                                        >  1:1386204/1‑140 (MQ=255)

 ctctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttt                                                                                                                                       >  1:563297/1‑140 (MQ=255)

 ctctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttt                                                                                                                                       <  2:1200942/140‑1 (MQ=255)

 ctctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttt                                                                                                                                       >  2:89455/1‑140 (MQ=255)

   ctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttttt                                                                                                                                     >  1:182922/1‑140 (MQ=255)

   ctTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAttttt                                                                                                                                     >  2:1285031/1‑140 (MQ=255)

    ttCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttt                                                                                                                                    >  2:1563322/1‑140 (MQ=255)

      cGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttc                                                                                                                                  <  1:718642/140‑2 (MQ=255)

       gTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttcc                                                                                                                                 <  1:911595/140‑3 (MQ=255)

        tACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttcca                                                                                                                                <  1:459514/140‑4 (MQ=255)

         aCAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCAtttttttccag                                                                                                                               <  2:1285610/140‑5 (MQ=255)

             gATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGctct                                                                                                                          <  2:344814/140‑1 (MQ=255)

              aTGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtg                                                                                                                         <  2:1189715/140‑1 (MQ=255)

              aTGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtg                                                                                                                         <  2:808253/140‑1 (MQ=255)

                gCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtgtg                                                                                                                       <  2:332814/140‑1 (MQ=255)

                gCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtgtg                                                                                                                       >  1:1230738/1‑140 (MQ=255)

                gCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtgtg                                                                                                                       >  2:1469916/1‑140 (MQ=255)

                gCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCtgtg                                                                                                                       >  2:1437692/1‑140 (MQ=255)

                 cTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGa                                                                                                                      >  2:287497/1‑140 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  2:1382785/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  1:287497/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  1:953587/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  1:631597/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  1:139028/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  2:757635/140‑1 (MQ=255)

                   cATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGAcc                                                                                                                    <  2:896474/140‑1 (MQ=255)

                     tGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCtt                                                                                                                  >  2:1191876/1‑140 (MQ=255)

                     tGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCtt                                                                                                                  >  2:113844/1‑140 (MQ=255)

                     tGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCtt                                                                                                                  >  1:1240032/1‑140 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTGCCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGGCCTTGTc                                                                                                               <  2:180362/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  2:1370963/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  2:1185614/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  1:1032498/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  2:753146/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  1:662275/140‑1 (MQ=255)

                        gTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTc                                                                                                               <  1:462135/140‑1 (MQ=255)

                              ttCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGtt                                                                                                         >  2:148678/1‑140 (MQ=255)

                                cGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTaa                                                                                                       >  2:1127198/1‑140 (MQ=255)

                                 gCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAAc                                                                                                      <  2:609835/140‑1 (MQ=255)

                                  cTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACt                                                                                                     <  2:845195/140‑1 (MQ=255)

                                  cTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACt                                                                                                     <  2:478956/140‑1 (MQ=255)

                                       ttgtgttgtgTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATg                                                                                                <  2:357176/140‑1 (MQ=255)

                                           gttgtgtGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTaa                                                                                            >  1:1411517/1‑140 (MQ=255)

                                            ttgtgtGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTaaa                                                                                           <  2:773617/140‑1 (MQ=255)

                                               tgtgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTg                                                                                        >  2:316067/1‑140 (MQ=255)

                                               tgtgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTg                                                                                        >  2:593187/1‑140 (MQ=255)

                                                gtgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGa                                                                                       <  2:1554401/140‑1 (MQ=255)

                                                 tgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAAt                                                                                          <  2:673435/136‑1 (MQ=255)

                                                 tgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGAt                                                                                      >  2:622506/1‑140 (MQ=255)

                                                 tgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGAt                                                                                      >  2:706652/1‑140 (MQ=255)

                                                 tgGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGAt                                                                                      >  2:868144/1‑140 (MQ=255)

                                                   gCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGt                                                                                    <  1:1550176/140‑1 (MQ=255)

                                                      gCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAAc                                                                                 <  1:1324535/140‑1 (MQ=255)

                                                      gCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAAc                                                                                 >  2:311991/1‑140 (MQ=255)

                                                         gATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATa                                                                              >  1:270444/1‑140 (MQ=255)

                                                         gATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTAACGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAAt                                                                                          >  1:561023/1‑128 (MQ=255)

                                                         gATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTAACGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAAt                                                                                          <  2:561023/128‑1 (MQ=255)

                                                           tttttGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAAt                                                                            <  1:1563322/140‑1 (MQ=255)

                                                           tttttGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAAt                                                                            <  2:1246231/140‑1 (MQ=255)

                                                           tttttGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTCCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAAt                                                                            <  2:1162737/140‑1 (MQ=255)

                                                           tttttGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCCACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAAt                                                                            <  2:1295641/140‑1 (MQ=255)

                                                             tttGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCa                                                                          <  1:272522/140‑1 (MQ=255)

                                                               tGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACt                                                                        <  2:1368679/140‑1 (MQ=255)

                                                                    gctgctTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGt                                                                   <  1:132917/140‑1 (MQ=255)

                                                                        ctTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGAtg                                                               <  1:624137/140‑1 (MQ=255)

                                                                                  cAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGtttt                                                     >  2:1522230/1‑140 (MQ=255)

                                                                                  cAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGtttt                                                     >  2:1090875/1‑140 (MQ=255)

                                                                                     ttattaAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTCCCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTAGTCTTGGTTAACTCAATGTCAAATTGATGAAACATAATCACTTACGGGATGTGCGTGTTTTGcc                                                  >  2:561212/1‑139 (MQ=255)

                                                                                     ttattaAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCg                                                  >  2:1206582/1‑140 (MQ=255)

                                                                                     ttattaAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCg                                                  >  2:264427/1‑140 (MQ=255)

                                                                                     ttattaAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCg                                                  >  1:494450/1‑140 (MQ=255)

                                                                                        ttaAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGt                                               >  1:411894/1‑140 (MQ=255)

                                                                                         taAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGtt                                              <  2:182922/140‑1 (MQ=255)

                                                                                           aGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTaa                                            >  1:1311922/1‑140 (MQ=255)

                                                                                             cGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGa                                          >  1:1435904/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTTAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGGCTTGGTTAATTCAATGTTAAATTGTTGACACATAAGCAATTACGTGCAGTGCGGGTTTTGCGAGTTAAGaaa                                        >  2:1387755/1‑139 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  1:599237/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  1:124618/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  2:285342/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  1:409686/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  1:39498/1‑140 (MQ=255)

                                                                                               ggCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAAc                                        >  2:1156150/1‑140 (MQ=255)

                                                                                                 cTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAg                                      <  2:563297/140‑1 (MQ=255)

                                                                                                 cTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAg                                      >  1:1054003/1‑140 (MQ=255)

                                                                                                            cgtCTATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTc                           <  1:1191876/140‑1 (MQ=255)

                                                                                                                tATCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTAc                       <  2:235643/140‑1 (MQ=255)

                                                                                                                 aTCTCTTTAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTAcc                      <  1:1127198/140‑1 (MQ=255)

                                                                                                                       ttAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCa                <  2:604071/140‑1 (MQ=255)

                                                                                                                       ttAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCa                >  2:796626/1‑140 (MQ=255)

                                                                                                                       ttAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCa                >  1:618913/1‑140 (MQ=255)

                                                                                                                       ttAGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCa                <  1:1090875/140‑1 (MQ=255)

                                                                                                                         aGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACg              <  1:39219/140‑1 (MQ=255)

                                                                                                                         aGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACg              <  1:113844/140‑1 (MQ=255)

                                                                                                                         aGCTACCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACg              <  1:285342/140‑1 (MQ=255)

                                                                                                                             aCCGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGt          <  1:337798/140‑1 (MQ=255)

                                                                                                                              ccGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTc         >  1:653507/1‑140 (MQ=255)

                                                                                                                              ccGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTc         >  1:522596/1‑140 (MQ=255)

                                                                                                                               cGGTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTcc        <  1:1522230/140‑1 (MQ=255)

                                                                                                                                ggTCAGACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCg       >  2:807034/1‑140 (MQ=255)

                                                                                                                                     gACCA‑TTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAAt  >  1:1289504/1‑140 (MQ=255)

GCTCTTCGTACAAGATGCTCATGAGTAGGCTTCGCTTTGTTGTGTTGTGTGGCAGCTGATTTTTGCGCGCTGCTTCTGTGAACAGTTATTAAGCGGGCTTTTCGTTTTCGTCTATCTCTTTAGCTACCGGTCAGACCATTTTTTTTCCAGCTCTGTGACCTTGTCTTGGTTAACTCAATGTTAAATTGATGTAACATAATCACTTACGTGATGTGCGTGTTTTGCGAGTTAAGAACAGAAAAATTGGTCCTACCTGTGCACGAGGTCCGGGAAT  >  NC_000913/3128000‑3128273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`