Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,037,809 (GT)5→4 noncoding (291‑292/2905 nt) rrlA → 23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,8010G.98.2% 219.1 / 2.0 109noncoding (283/2905 nt)rrlA23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (40/67);  total (41/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.61e-01
*NC_0009134,037,8020T.98.2% 217.2 / 2.6 109noncoding (284/2905 nt)rrlA23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base . (40/67);  total (41/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 110 total aligned reads displayed.

GAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTC  >  NC_000913/4037693‑4037938
                                                                                                            ||                                                                                                                                        
gAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGt                                                                                                          <  2:555700/140‑1 (MQ=2)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:1282822/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:1175754/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:427784/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:431510/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  1:1477710/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:816577/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  1:96204/140‑1 (MQ=9)
 aGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTg                                                                                                         <  2:295464/140‑1 (MQ=9)
   gCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCg                                                                                                       >  2:1537714/1‑140 (MQ=12)
    cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGa                                                                                                      >  1:1273947/1‑140 (MQ=12)
    cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGa                                                                                                      >  2:307208/1‑140 (MQ=12)
    cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGa                                                                                                      >  2:55590/1‑140 (MQ=12)
    cGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGa                                                                                                      >  2:489123/1‑140 (MQ=12)
      aaCCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATa                                                                                                    <  2:687876/140‑1 (MQ=16)
            gggAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAgggg                                                                                              >  2:1052826/1‑139 (MQ=12)
              gAACTGAACCATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGa                                                                                            <  2:436977/140‑1 (MQ=255)
              gAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGa                                                                                            <  1:4724/140‑1 (MQ=16)
              gAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGa                                                                                            <  1:349647/140‑1 (MQ=16)
                aCTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACa                                                                                          >  2:329995/1‑140 (MQ=12)
                 cTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAg                                                                                         >  1:1208623/1‑140 (MQ=12)
                   gAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGcc                                                                                       >  2:1559637/1‑140 (MQ=12)
                   gAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGcc                                                                                       >  1:224020/1‑140 (MQ=12)
                   gAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGcc                                                                                       <  2:78185/140‑1 (MQ=16)
                          cTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAACCCCGTaca                                                                                >  1:799482/1‑140 (MQ=255)
                              gTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACaaa                                                                            >  2:875893/1‑140 (MQ=12)
                              gTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACaaa                                                                            >  2:1373372/1‑140 (MQ=12)
                              gTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACaaa                                                                            >  1:825157/1‑140 (MQ=12)
                                aCCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACaaaaa                                                                          <  2:596384/140‑1 (MQ=16)
                                  cccGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATg                                                                        >  1:1139983/1‑140 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATg                                                                        >  1:1543097/1‑138 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATg                                                                        <  2:1543097/138‑1 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcc                                                                      >  2:1311980/1‑139 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGca                                                                      >  1:507354/1‑140 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGca                                                                      <  2:837725/140‑1 (MQ=16)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGca                                                                      >  1:60100/1‑140 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGca                                                                      >  2:1295935/1‑140 (MQ=12)
                                    cGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGca                                                                      >  2:936865/1‑140 (MQ=12)
                                     gAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGTCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcac                                                                     <  2:1456663/140‑1 (MQ=255)
                                     gAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcac                                                                     >  1:967526/1‑140 (MQ=12)
                                     gAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcac                                                                     <  1:794264/140‑1 (MQ=16)
                                      aGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcaca                                                                    <  2:768138/140‑1 (MQ=16)
                                      aGGAAAAGAAATCAACCGAGATGCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGcaca                                                                    <  1:850498/140‑1 (MQ=255)
                                       ggAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACAt                                                                   >  1:445102/1‑140 (MQ=12)
                                             gAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCtgtg                                                             <  2:123243/140‑1 (MQ=16)
                                              aaaTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGa                                                            <  2:894891/140‑1 (MQ=16)
                                              aaaTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGa                                                            <  1:1052826/140‑1 (MQ=16)
                                              aaaTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGa                                                            <  1:240720/140‑1 (MQ=16)
                                               aaTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAg                                                           <  2:326610/140‑1 (MQ=12)
                                                aTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                                          >  1:226628/1‑140 (MQ=12)
                                                aTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                                          >  1:453462/1‑140 (MQ=12)
                                                aTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                                          >  1:1577572/1‑140 (MQ=12)
                                                aTCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGc                                                          <  1:570699/140‑1 (MQ=12)
                                                  cAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTc                                                        >  1:50766/1‑140 (MQ=12)
                                                   aaCCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCg                                                       <  2:146043/140‑1 (MQ=12)
                                                   aaCCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCg                                                       <  2:414496/140‑1 (MQ=12)
                                                   aaCCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCg                                                       <  1:1161238/140‑1 (MQ=12)
                                                     ccGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGTACATGCTGTGAGCTCGAt                                                     >  2:667488/1‑140 (MQ=255)
                                                     ccGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAACCTGAATCAG‑G‑GTGAGTTAGGGGAACCGTCTGGAAAGGCGTGCGATAAAGGGGGACAGCCCCGGACACAAAAATGAACAAGCTGTGAGCTCGaa                                                     >  2:783278/1‑139 (MQ=11)
                                                          aTTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTa                                                <  2:216220/140‑1 (MQ=12)
                                                             cccccAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAggg                                             >  1:603725/1‑140 (MQ=12)
                                                             cccccAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAggg                                             <  2:201182/140‑1 (MQ=12)
                                                               cccAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCg                                           <  2:1259440/140‑1 (MQ=12)
                                                                ccAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCgg                                          <  1:1373372/140‑1 (MQ=12)
                                                                 cAGTAGCGGCGAGCGAACGGTGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCggg                                         <  2:411156/140‑1 (MQ=255)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  2:936756/140‑1 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       >  2:1075740/1‑140 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  1:4449/140‑1 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       >  2:893232/1‑140 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  1:875893/140‑1 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  2:1537917/140‑1 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  2:606679/140‑1 (MQ=12)
                                                                   gTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGAGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGac                                       <  2:1150567/140‑1 (MQ=255)
                                                                       cggcgAGCGAACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGt                                   <  1:1176515/140‑1 (MQ=12)
                                                                         gcgagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTgg                                 >  1:768812/1‑140 (MQ=12)
                                                                         gcgagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTgg                                 >  2:429909/1‑140 (MQ=12)
                                                                          cgagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGt                                <  1:508665/140‑1 (MQ=12)
                                                                          cgagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGt                                >  1:1256333/1‑140 (MQ=12)
                                                                           gagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCTCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTa                               <  1:1156177/140‑1 (MQ=255)
                                                                           gagcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTa                               <  1:1036687/140‑1 (MQ=12)
                                                                             gcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATc                             <  2:1322816/140‑1 (MQ=12)
                                                                             gcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATc                             <  2:696949/140‑1 (MQ=12)
                                                                             gcgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATc                             <  1:87344/140‑1 (MQ=12)
                                                                              cgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATcc                            >  1:967763/1‑140 (MQ=12)
                                                                              cgaACGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATcc                            <  1:489101/140‑1 (MQ=12)
                                                                                 aCGGGGAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGt                         <  2:72431/140‑1 (MQ=12)
                                                                                      gAGCAGCCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGTTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGaa                    <  1:1559637/140‑1 (MQ=255)
                                                                                       aggagcCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGa                     >  2:721870/4‑140 (MQ=1)
                                                                                       aacagcCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAat                   >  1:1094201/3‑140 (MQ=12)
                                                                                           gcCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATgg               >  2:115154/1‑140 (MQ=12)
                                                                                           gcCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATgg               >  2:1179342/1‑140 (MQ=12)
                                                                                           gcCCAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTAAACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATgg               <  2:1179602/140‑1 (MQ=255)
                                                                                              cAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggggg            <  1:893232/140‑1 (MQ=12)
                                                                                              cAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggggg            <  1:262239/140‑1 (MQ=12)
                                                                                              cAGAGCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATggggg            >  1:1166083/1‑140 (MQ=12)
                                                                                                gagCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGa          <  1:783278/140‑1 (MQ=12)
                                                                                                 agCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGAc         <  1:789358/140‑1 (MQ=12)
                                                                                                 agCCTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGAc         <  1:329995/140‑1 (MQ=12)
                                                                                                    cTGAATCA‑‑GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCAt      <  1:634812/140‑1 (MQ=12)
                                                                                                         tCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATAT‑GGGGGACCAtcctc  <  1:721870/140‑1 (MQ=17)
                                                                                                            ||                                                                                                                                        
GAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGTGCGATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTC  >  NC_000913/4037693‑4037938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑