Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,152	(C)5→6	coding (816/2463 nt)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147	1	.	C	100.0%	341.4 / NA	91	T271P (ACC→CCC)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (44/47);  total (44/47)

TTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACA  >  NC_000913/1010‑1279                                                                                                                                           |                                                                                                                                      tttGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑cc                                                                                                                                    <  2:1215672/140‑1 (MQ=255) tttGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑cc                                                                                                                                    >  1:789988/1‑140 (MQ=255) tttGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑cc                                                                                                                                    >  1:780742/1‑140 (MQ=255)      acggacgTTGACGGGGTCTATACCTGCGGCCCGCGTCAGGTGGCCGAGGCGAGGTTGTTGAAGACGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCGCGCCCGCACCATTA‑‑cccccca                                                                                                                               <  2:275901/140‑3 (MQ=255)      acggacgTTGACGGGGTCTATACCTGCGACCCGCGTCAGTTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑cccccca                                                                                                                               <  2:1172884/140‑3 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  1:1030786/140‑1 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           >  2:461008/1‑140 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  2:420355/140‑1 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  1:1359496/140‑1 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  2:946773/140‑1 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  2:1200161/140‑1 (MQ=255)           cgTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGcc                                                                                                                           <  1:327339/140‑1 (MQ=255)            gTTGACGGGGTCTATACCTGCGCCCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGccc                                                                                                                          <  1:197573/140‑1 (MQ=255)            gTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGccc                                                                                                                          <  2:594547/140‑1 (MQ=255)                 cGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTc                                                                                                                     <  1:276671/140‑1 (MQ=255)                   gggTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCa                                                                                                                   <  2:1032487/140‑1 (MQ=255)                    ggTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAg                                                                                                                  >  1:1545365/1‑140 (MQ=255)                    ggTCTATACCTGCGACCCGCGTCAGGTGCACGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAg                                                                                                                  >  2:70577/1‑140 (MQ=255)                        tataCCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCCATCGCCCAGTTCCAGATc                                                                                                               >  1:1571870/1‑140 (MQ=255)                        tataCCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATcc                                                                                                              >  2:1229170/1‑140 (MQ=255)                          taCCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCt                                                                                                            >  2:440493/1‑140 (MQ=255)                            ccTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACCTCGGCGATAGGCTGCTTCAGTCCCGCACCATTA‑‑CCCCCATCGCCCAGTTCCAGATCCCGTGc                                                                                                         <  2:206935/140‑1 (MQ=255)                              tGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGcc                                                                                                        <  1:623780/140‑1 (MQ=255)                               gCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCt                                                                                                       >  2:715222/1‑140 (MQ=255)                               gCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCt                                                                                                       >  2:1484196/1‑140 (MQ=255)                                cGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTg                                                                                                      >  1:263735/1‑140 (MQ=255)                                cGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTg                                                                                                      >  1:1581931/1‑140 (MQ=255)                                cGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTg                                                                                                      >  1:1235006/1‑140 (MQ=255)                                   cccGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGAtt                                                                                                   <  2:1335068/140‑1 (MQ=255)                                    ccGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTa                                                                                                  >  2:1071769/1‑140 (MQ=255)                                    ccGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTa                                                                                                  >  2:337443/1‑140 (MQ=255)                                        gTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTaaaaa                                                                                              <  2:802234/140‑1 (MQ=255)                                               gCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGa                                                                                       <  2:649555/140‑1 (MQ=255)                                                 ccGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGaaa                                                                                     <  2:780742/140‑1 (MQ=255)                                                 ccGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGaaa                                                                                     >  1:939837/1‑140 (MQ=255)                                                 ccGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCACACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGaaa                                                                                     <  2:112690/140‑1 (MQ=255)                                                  cGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAAt                                                                                    >  2:803704/1‑140 (MQ=255)                                                  cGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAAt                                                                                    >  2:1046209/1‑140 (MQ=255)                                                  cGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAAt                                                                                    >  1:418522/1‑140 (MQ=255)                                                     tGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCt                                                                                 >  1:390767/1‑140 (MQ=255)                                                       cGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCa                                                                               <  2:367389/140‑1 (MQ=255)                                                       cGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCa                                                                               >  2:1356697/1‑140 (MQ=255)                                                       cGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCa                                                                               <  2:1318732/140‑1 (MQ=255)                                                          ggTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGc                                                                            <  2:659936/140‑1 (MQ=255)                                                           gttgttGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCa                                                                           >  1:774337/1‑140 (MQ=255)                                                           gttgttGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCa                                                                           <  2:718507/140‑1 (MQ=255)                                                            ttgttgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCAc                                                                          >  1:1423499/1‑140 (MQ=255)                                                               ttgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAg                                                                       >  1:216386/1‑140 (MQ=255)                                                                tgAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAgg                                                                      <  1:440493/140‑1 (MQ=255)                                                                 gAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGt                                                                     <  2:501684/140‑1 (MQ=255)                                                                    gTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACg                                                                  >  2:148352/1‑140 (MQ=255)                                                                      cGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCt                                                                >  1:1075017/1‑140 (MQ=255)                                                                      cGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCt                                                                <  1:1488383/140‑1 (MQ=255)                                                                        aTGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCa                                                              >  1:638391/1‑140 (MQ=255)                                                                        aTGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCa                                                              >  1:363798/1‑140 (MQ=255)                                                                         tGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCAt                                                             >  2:1181257/1‑140 (MQ=255)                                                                          gTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCAtt                                                            <  1:715222/140‑1 (MQ=255)                                                                          gTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCAtt                                                            <  2:939837/140‑1 (MQ=255)                                                                               aCCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGc                                                       >  2:1542027/1‑140 (MQ=255)                                                                                ccAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGcc                                                      <  1:1229170/140‑1 (MQ=255)                                                                                   ggAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGc                                                   >  2:906461/1‑140 (MQ=255)                                                                                    gAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGcc                                                  <  2:107619/140‑1 (MQ=255)                                                                                      agagATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGt                                                >  1:913912/4‑140 (MQ=255)                                                                                       gCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGtg                                               <  2:118263/140‑1 (MQ=255)                                                                                       gCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGtg                                               <  2:1187706/140‑1 (MQ=255)                                                                                        cGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGtga                                              >  1:846746/1‑140 (MQ=255)                                                                                         gATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGtgat                                             <  2:1235006/140‑1 (MQ=255)                                                                                                   tCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAAt                                   >  1:128835/1‑140 (MQ=255)                                                                                                      tACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTAc                                <  2:1419607/140‑1 (MQ=255)                                                                                                      tACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTAc                                <  2:484415/140‑1 (MQ=255)                                                                                                      tACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTAc                                <  2:390767/140‑1 (MQ=255)                                                                                                        cTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCg                              >  1:916141/1‑140 (MQ=255)                                                                                                          tCGGCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGt                            >  1:217323/1‑140 (MQ=255)                                                                                                            ggCGCTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCa                          <  2:263735/140‑1 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTc                           >  1:704265/1‑138 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTc                           <  2:704265/138‑1 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa                         >  2:1568817/1‑140 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa                         >  2:1005654/1‑140 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa                         >  1:560186/1‑140 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa                         >  1:357768/1‑140 (MQ=255)                                                                                                             gcgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCaa                         >  2:1053010/1‑140 (MQ=255)                                                                                                              cgcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAg                        >  1:618660/1‑140 (MQ=255)                                                                                                               gcTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAgg                       >  2:22546/1‑140 (MQ=255)                                                                                                                cTAAAGTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAACCGTGATGAAGACGAATTACCGGTCAAggg                      <  1:1046209/140‑1 (MQ=255)                                                                                                                     gTTCTTCACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCAttt                 <  2:128835/140‑1 (MQ=255)                                                                                                                        cttcACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCa              <  2:263679/140‑1 (MQ=255)                                                                                                                        cttcACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCa              <  1:751882/140‑1 (MQ=255)                                                                                                                          tcACCCCCGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAAt            <  2:638391/140‑1 (MQ=255)                                                                                                                             cccccGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTg         <  1:931955/140‑1 (MQ=255)                                                                                                                               cccGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGaa       <  2:1581931/140‑1 (MQ=255)                                                                                                                               cccGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGaa       <  2:1075017/140‑1 (MQ=255)                                                                                                                               cccGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGaa       <  2:98936/140‑1 (MQ=255)                                                                                                                                ccGCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAAt      >  1:395972/1‑140 (MQ=255)                                                                                                                                  gCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATaa    >  1:717612/1‑140 (MQ=255)                                                                                                                                  gCACCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATaa    <  1:148352/140‑1 (MQ=255)                                                                                                                                    aCCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACa  <  2:789988/140‑1 (MQ=255)                                                                                                                                    aCCATTAC‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACa  <  2:800024/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                      TTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTA‑‑CCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACA  >  NC_000913/1010‑1279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑