Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,691	0	T	G	25.6%	202.1 / 14.9	86	intergenic (+24/‑140)	ymiC/acnA	protein YmiC/aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base T (15/49);  new base G (22/0);  total (37/49)
Fisher's exact test for biased strand distribution p-value = 5.51e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCT  >  NC_000913/1335558‑1335830                                                                                                                                      |                                                                                                                                            tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAt                                                                                                                                       <  1:339746/140‑1 (MQ=255) tAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAt                                                                                                                                       >  1:667700/1‑140 (MQ=255)       tgtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGTCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctt                                                                                                                                 <  2:478138/140‑1 (MQ=255)        gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgcttt                                                                                                                                >  2:560922/1‑140 (MQ=255)        gtAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgcttt                                                                                                                                >  1:1483527/1‑140 (MQ=255)         tAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCAtttgctttg                                                                                                                               >  1:753054/1‑140 (MQ=255)          aGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCAtttgctttgc                                                                                                                              >  2:381720/1‑140 (MQ=255)           gCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGcc                                                                                                                             >  1:826570/1‑140 (MQ=255)             cTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCcac                                                                                                                           <  1:610673/140‑1 (MQ=255)             cTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCcac                                                                                                                           <  1:442567/140‑1 (MQ=255)             cTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTATTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCcac                                                                                                                           <  2:403912/140‑1 (MQ=255)                tGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAg                                                                                                                        >  2:1184839/1‑140 (MQ=255)                tGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAg                                                                                                                        >  2:956435/1‑140 (MQ=255)                 gATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAgg                                                                                                                       >  1:233118/1‑140 (MQ=255)                  aTCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGt                                                                                                                      <  2:363436/140‑1 (MQ=255)                     aaCACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtc                                                                                                                   >  2:869900/1‑140 (MQ=255)                      acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGTTTTGCCACAAGGTTtct                                                                                                                  >  1:893171/1‑140 (MQ=255)                      acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  <  1:1400036/140‑1 (MQ=255)                      acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  <  1:601047/140‑1 (MQ=255)                      acacAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTtct                                                                                                                  >  2:1245010/1‑140 (MQ=255)                       cacaAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTtctc                                                                                                                 >  1:1083597/1‑140 (MQ=255)                            atatGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGCCGGGGTTGTCTTTTCTTTTTCCACAAGGTTTTTCCTCtt                                                                                                            >  2:256339/1‑140 (MQ=255)                                    tatTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAt                                                                                                    <  2:208947/140‑1 (MQ=255)                                     atTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAtt                                                                                                   <  2:1226266/140‑1 (MQ=255)                                      caggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAAttt                                                                                                  <  1:764643/138‑1 (MQ=255)                                        ggTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTgg                                                                                                >  2:234820/1‑140 (MQ=255)                                         gTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTggg                                                                                               <  1:560922/140‑1 (MQ=255)                                         gTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTggg                                                                                               <  1:381720/140‑1 (MQ=255)                                         gTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTggg                                                                                               <  1:869900/140‑1 (MQ=255)                                          tCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgt                                                                                              <  1:1132769/140‑1 (MQ=255)                                           ccTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTTATTTGCTTTTCCACAAGGGTTCTCCTCTTTTATCAATTTGGgtt                                                                                             >  1:1025319/1‑140 (MQ=255)                                            cTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGgttg                                                                                            >  2:1184896/1‑140 (MQ=255)                                                   ggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaa                                                                                     >  2:252656/1‑140 (MQ=255)                                                   ggCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCCTTTTCTTTTCCACAAGGGTTCTCCTCTTTTATCAATTTGGGTTGTTATCaa                                                                                     >  2:1067240/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGGGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                    >  1:161179/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCCTTTGCTTTGCCACAAGGATTCTCCTCTTTTTTCAATTTGGGGTGTTTTcctg                                                                                    >  2:764643/1‑137 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                    >  1:586630/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                    >  1:765181/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCCTTTGCTTTGCCACAAGGGTTCTCCTCTTTTTTCAATTTGGGTTGTTTTCaaa                                                                                    >  2:863629/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCCCAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCaaa                                                                                    >  2:590699/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCaaa                                                                                    >  1:1198783/1‑140 (MQ=255)                                                    gcgcGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCaaa                                                                                    >  1:1242083/1‑140 (MQ=255)                                                      gcgTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                                                                  <  2:1483527/140‑1 (MQ=255)                                                      gcgTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATc                                                                                  <  2:753054/140‑1 (MQ=255)                                                         ttttCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAAGTTTCTCCTCTTTTATCAATTTTGGGTGTTTTCAAATCGtt                                                                               >  2:1553711/1‑140 (MQ=255)                                                            tctcTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  1:281010/140‑1 (MQ=255)                                                            tctcTGTCGATGCTCTTCTCGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcg                                                                            <  1:1184839/140‑1 (MQ=255)                                                              tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                                                                          <  1:1171022/140‑1 (MQ=255)                                                              tctGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTAcgcg                                                                          <  1:302442/140‑1 (MQ=255)                                                                 gTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATg                                                                       <  2:1089132/140‑1 (MQ=255)                                                                 gTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATg                                                                       <  2:1083597/140‑1 (MQ=255)                                                                       gCTCTTCTGGGCCGACCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                                                                 <  1:972648/140‑1 (MQ=255)                                                                       gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGGGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                                                                 <  2:161179/140‑1 (MQ=255)                                                                       gCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTtgtg                                                                 <  1:1491872/140‑1 (MQ=255)                                                                         tcttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGtt                                                               <  1:1462924/140‑1 (MQ=255)                                                                          cttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTa                                                              <  1:252656/140‑1 (MQ=255)                                                                           ttctGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTAt                                                             <  2:375002/140‑1 (MQ=255)                                                                               gggCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCttt                                                         <  1:181713/140‑1 (MQ=255)                                                                                  ccGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAat                                                      <  1:420239/140‑1 (MQ=255)                                                                                    gAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAatat                                                    <  2:842976/140‑1 (MQ=255)                                                                                     aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   <  1:1245010/140‑1 (MQ=255)                                                                                     aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTTCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAAACGTTACGCGGTGGTTGTGTTATCTTTAATAtt                                                   >  2:1218989/1‑140 (MQ=255)                                                                                     aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  1:1050482/1‑140 (MQ=255)                                                                                     aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  1:1220876/1‑140 (MQ=255)                                                                                     aaCTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATAtt                                                   >  1:1226463/1‑140 (MQ=255)                                                                                         cctcTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAcc                                               <  1:538521/140‑1 (MQ=255)                                                                                         cctcTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCAcc                                               <  1:590699/140‑1 (MQ=255)                                                                                            ctGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTg                                            >  1:707114/1‑140 (MQ=255)                                                                                              ggATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGaa                                          <  2:170166/140‑1 (MQ=255)                                                                                               gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAag                                         <  2:70203/140‑1 (MQ=255)                                                                                               gATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAag                                         <  2:893171/140‑1 (MQ=255)                                                                                                    ttACTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAAt                                    >  1:209586/1‑140 (MQ=255)                                                                                                       cTCACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAg                                 >  1:266194/1‑140 (MQ=255)                                                                                                         cACTGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAggg                               >  1:1129602/1‑140 (MQ=255)                                                                                                            tGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTTCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCtt                            >  2:912742/1‑140 (MQ=255)                                                                                                            tGATCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCtt                            >  1:136610/1‑140 (MQ=255)                                                                                                             gATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTTCCACAAGGGTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTc                           >  1:487655/1‑140 (MQ=255)                                                                                                              aTCCTTGACCCCGCTGCGGCGGGGTTGTTATTTTTTTTGCCACAAGGTTTCTCCTCTTTTATCCATTTGGGGTGTTTTCAAAACGTTACGCCATGCTTTGGGTATTTTTAAAATTTTCCCCGATGAGAAATACGGCtttg                          >  2:416322/1‑138 (MQ=255)                                                                                                              aTCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCg                          <  2:233118/140‑1 (MQ=255)                                                                                                              aTCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCCCAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCg                          >  1:1051787/1‑140 (MQ=255)                                                                                                              aTCCTTGACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCg                          >  2:1299644/1‑140 (MQ=255)                                                                                                               tCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTTCCACAAGGTTTTTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGc                         >  2:334261/1‑140 (MQ=255)                                                                                                                 cTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCaa                       <  1:863629/140‑1 (MQ=255)                                                                                                                   tGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAcc                     <  1:1553711/140‑1 (MQ=255)                                                                                                                    gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    <  2:765181/140‑1 (MQ=255)                                                                                                                    gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    <  1:453752/140‑1 (MQ=255)                                                                                                                    gACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    <  1:234820/140‑1 (MQ=255)                                                                                                                    gACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    >  1:807282/1‑140 (MQ=255)                                                                                                                    gACCCCGCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGGTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAAccc                    >  2:1225349/1‑140 (MQ=255)                                                                                                                      ccccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATATTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTg                  <  1:1067240/140‑1 (MQ=255)                                                                                                                      ccccGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCCCTTTTTTCAATTTGGGTTGTTTTCAAATCGTTACCCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTg                  >  2:603355/1‑140 (MQ=255)                                                                                                                        ccGCTGCGGCGGGGGTGTCATTTGCTTTTCCCCAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTc                >  2:1367902/1‑140 (MQ=255)                                                                                                                          gCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              <  1:1218989/140‑1 (MQ=255)                                                                                                                          gCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGTTTCCCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              >  2:774005/1‑140 (MQ=255)                                                                                                                          gCTGCGGCGGGGGTGTCATTTGCTTTGCCACAAGGGTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAt              >  1:990391/1‑140 (MQ=255)                                                                                                                           cTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCAtt             <  2:826570/140‑1 (MQ=255)                                                                                                                               ggcggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg         <  2:586630/140‑1 (MQ=255)                                                                                                                               ggcggGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAagg         <  1:256339/140‑1 (MQ=255)                                                                                                                                      ttGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGACTTCGCAACCCTGTCATTAAGGAGGAGCt  <  1:956435/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                            TAAGGGTGTAGCCTATGATCAACACAAATATGAAATATTGGTCCTGGATGGGCGCGTTTTCTCTGTCGATGCTCTTCTGGGCCGAACTCCTCTGGATCATTACTCACTGATCCTTGACCCCGCTGCGGCGGGGTTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCT  >  NC_000913/1335558‑1335830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑