Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,152	T→C	D97D (GAT→GAC)	tabA →	DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,152	0	T	C	100.0%	332.0 / NA	87	D97D (GAT→GAC)	tabA	DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/39);  total (48/39)

ATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAG  >  NC_000913/4475015‑4475288                                                                                                                                          |                                                                                                                                         atGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACaa                                                                                                                                        >  1:633793/1‑140 (MQ=255)   gACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAg                                                                                                                                      <  1:1048365/140‑1 (MQ=255)        aGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATc                                                                                                                                 >  1:106083/1‑140 (MQ=255)        aGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATc                                                                                                                                 >  1:144537/1‑140 (MQ=255)        aGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCATGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATc                                                                                                                                 >  1:3737/1‑140 (MQ=255)         gCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCg                                                                                                                                >  2:24018/1‑140 (MQ=255)          ccGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGc                                                                                                                               >  1:444630/1‑140 (MQ=255)                 aaGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTg                                                                                                                        <  2:969335/140‑1 (MQ=255)                   gCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGcc                                                                                                                      >  1:296503/1‑140 (MQ=255)                    cTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCg                                                                                                                     >  1:377975/1‑140 (MQ=255)                       gCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCTGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGaa                                                                                                                  >  2:1161199/1‑140 (MQ=255)                        ccGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAg                                                                                                                 <  1:1191530/140‑1 (MQ=255)                           tGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCg                                                                                                              <  1:1042691/140‑1 (MQ=255)                                 gTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTtgatg                                                                                                        <  1:983167/140‑1 (MQ=255)                                   aCCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACC‑TCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATgaga                                                                                                     >  1:442231/1‑140 (MQ=255)                                     cATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGaa                                                                                                    <  2:1432997/140‑1 (MQ=255)                                       tGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGaaaa                                                                                                  >  2:1496925/1‑140 (MQ=255)                                       tGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGaaaa                                                                                                  >  1:893073/1‑140 (MQ=255)                                          ccGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAg                                                                                               >  1:1488950/1‑140 (MQ=255)                                          ccGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAg                                                                                               <  1:1236269/140‑1 (MQ=255)                                           cGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGt                                                                                              <  1:795965/140‑1 (MQ=255)                                           cGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGt                                                                                              <  2:1064350/140‑1 (MQ=255)                                           cGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCATTGATGAGAAAACAGt                                                                                              <  1:1579101/140‑1 (MQ=255)                                            gCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGtt                                                                                             >  1:140581/1‑140 (MQ=255)                                            gCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGtt                                                                                             >  2:567890/1‑140 (MQ=255)                                            gCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGtt                                                                                             >  1:179869/1‑140 (MQ=255)                                               aTCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTAtt                                                                                          >  1:1256939/1‑140 (MQ=255)                                                      cATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATg                                                                                   <  2:106083/140‑1 (MQ=255)                                                        ttCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGaa                                                                                 >  1:721820/1‑140 (MQ=255)                                                        ttCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGaa                                                                                 >  2:1588032/1‑140 (MQ=255)                                                        ttCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGaa                                                                                 >  2:1445451/1‑140 (MQ=255)                                                          cAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAgg                                                                               >  1:307882/1‑140 (MQ=255)                                                          cAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAgg                                                                               >  1:949328/1‑140 (MQ=255)                                                          cAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAgg                                                                               <  1:895974/140‑1 (MQ=255)                                                          cAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAgg                                                                               >  2:971063/1‑140 (MQ=255)                                                            gATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTg                                                                             >  1:638475/1‑140 (MQ=255)                                                               tgtgTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCGGACAAAGGCATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGAtt                                                                          <  2:377615/140‑1 (MQ=255)                                                                 tgtTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGAtttt                                                                        >  1:636966/1‑140 (MQ=255)                                                                   ttAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTttgt                                                                      <  1:24018/140‑1 (MQ=255)                                                                       aaGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTttgttgag                                                                  >  1:231547/1‑138 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  2:1434652/1‑140 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  2:527236/1‑140 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  1:778355/1‑140 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  1:1089415/1‑140 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  1:1178281/1‑140 (MQ=255)                                                                           tCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCACCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGtttt                                                              >  2:126657/1‑140 (MQ=255)                                                                             aGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTAt                                                            >  1:1156958/1‑140 (MQ=255)                                                                                aaGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCg                                                         <  2:296503/140‑1 (MQ=255)                                                                                   gCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCgggg                                                      <  2:128247/140‑1 (MQ=255)                                                                                    cATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCggggg                                                     >  1:473351/1‑140 (MQ=255)                                                                                    cATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCggggg                                                     <  2:1074184/140‑1 (MQ=255)                                                                                      tGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGaa                                                   <  1:1331970/140‑1 (MQ=255)                                                                                        aCCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGt                                                 <  1:808556/140‑1 (MQ=255)                                                                                        aCCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGt                                                 <  2:3737/140‑1 (MQ=255)                                                                                        aCCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGGGATTTTGTTGTGTTTTATCCGGAGGAAgg                                                 >  2:520666/1‑139 (MQ=255)                                                                                          cTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGc                                               >  1:865453/1‑140 (MQ=255)                                                                                          cTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGc                                               <  1:340163/140‑1 (MQ=255)                                                                                          cTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGc                                               <  1:1496925/140‑1 (MQ=255)                                                                                             cAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATa                                            >  2:148262/1‑140 (MQ=255)                                                                                             cAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATa                                            >  2:977103/1‑140 (MQ=255)                                                                                               gCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATaaa                                          <  1:971063/140‑1 (MQ=255)                                                                                                  cGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCg                                       <  2:624015/140‑1 (MQ=255)                                                                                                  cGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCg                                       >  1:758374/1‑140 (MQ=255)                                                                                                       ccTGCAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCtgtg                                  >  1:414936/1‑140 (MQ=255)                                                                                                           cAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCa                              <  2:1488950/140‑1 (MQ=255)                                                                                                           cAGGCGCGCCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCa                              >  1:1491142/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  1:1438383/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  2:80367/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  1:259907/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  1:183342/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  1:1256559/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  1:1573161/1‑140 (MQ=255)                                                                                                                 cgcCGGATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGAGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgc                        >  2:941199/1‑140 (MQ=255)                                                                                                                     ggATACCGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGgcgcgc                    <  2:140581/140‑1 (MQ=255)                                                                                                                           cGACTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGccc              <  2:778355/140‑1 (MQ=255)                                                                                                                             aCTGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAg            <  1:307794/140‑1 (MQ=255)                                                                                                                               tGGTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGt          <  1:1445451/140‑1 (MQ=255)                                                                                                                                ggTTAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGtt         <  2:1581888/140‑1 (MQ=255)                                                                                                                                  ttAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCg       <  2:444630/140‑1 (MQ=255)                                                                                                                                  ttAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCg       <  2:633793/140‑1 (MQ=255)                                                                                                                                  ttAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCg       <  2:144537/140‑1 (MQ=255)                                                                                                                                  ttAGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCg       <  1:899285/140‑1 (MQ=255)                                                                                                                                    aGCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCa     <  2:377975/140‑1 (MQ=255)                                                                                                                                     gCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCaa    <  2:307882/140‑1 (MQ=255)                                                                                                                                     gCTGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCaa    <  1:148262/140‑1 (MQ=255)                                                                                                                                       tGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAg  <  1:126657/140‑1 (MQ=255)                                                                                                                                       tGACAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAg  <  1:520666/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ATGACCGAGCCGTACGAAGCTCGCCGTGCGGAGTACCATGCCCGCTATCTTGACATTCAGATTGTGTTAAAAGGTCAGGAAGGCATGACCTTCAGCACGCAACCTGCAGGCGCGCCGGATACCGACTGGTTAGCTGATAAAGACATCGCATTTTTGCCGGAAGGCGTTGATGAGAAAACAGTTATTCTAAATGAAGGTGATTTTGTTGTGTTTTATCCGGGGGAAGTGCATAAACCGCTGTGCGCAGTGGGCGCGCCAGCCCAGGTTCGCAAAG  >  NC_000913/4475015‑4475288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑