Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	286,748	C→T	P452S (CCC→TCC)	yagG →	putative D‑xylonate transporter YagG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	286,748	0	C	T	100.0%	292.1 / NA	85	P452S (CCC→TCC)	yagG	putative D‑xylonate transporter YagG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/36);  total (49/36)

TTCTGCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAG  >  NC_000913/286616‑286884                                                                                                                                     |                                                                                                                                         ttctgCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGAcgc                                                                                                                                   >  2:346545/1‑140 (MQ=255)  tctgCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGAcgcc                                                                                                                                  >  2:86385/1‑140 (MQ=255)      cGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGa                                                                                                                              >  2:171547/1‑140 (MQ=255)           tgCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGcc                                                                                                                         >  1:843396/1‑140 (MQ=255)              cGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGACCGTTTCCGACGCCGCGACAGCCGCa                                                                                                                      <  2:630338/140‑1 (MQ=255)               ggtggtGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCAt                                                                                                                     <  1:1133782/140‑1 (MQ=255)                   gtgCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCa                                                                                                                 <  2:1081358/140‑1 (MQ=255)                           cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGTCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCgg                                                                                                         >  2:1259357/1‑140 (MQ=255)                           cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCgg                                                                                                         >  2:301507/1‑140 (MQ=255)                           cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCgg                                                                                                         >  2:136745/1‑140 (MQ=255)                           cgcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCgg                                                                                                         >  1:618000/1‑140 (MQ=255)                            gcgGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGa                                                                                                        <  1:1307882/140‑1 (MQ=255)                              gggCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAgg                                                                                                      <  1:1235242/140‑1 (MQ=255)                                 cATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCaa                                                                                                   >  2:998118/1‑140 (MQ=255)                                 cATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCaa                                                                                                   >  2:470950/1‑140 (MQ=255)                                   tGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                 >  1:1481356/1‑140 (MQ=255)                                   tGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                 >  1:971746/1‑140 (MQ=255)                                   tGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                 >  1:72706/1‑140 (MQ=255)                                   tGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAata                                                                                                 >  1:544895/1‑140 (MQ=255)                                     ttCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGTCGCATCCCATTAACCGGAGGCAATATg                                                                                               >  2:617950/1‑140 (MQ=255)                                       catcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGa                                                                                             <  2:1581881/140‑1 (MQ=255)                                        atcatGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGaa                                                                                            <  2:1510127/140‑1 (MQ=255)                                            tGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCa                                                                                        >  1:1356171/1‑140 (MQ=255)                                             gCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCAc                                                                                       >  1:120698/1‑140 (MQ=255)                                              cTGTCGCTCTACAAGCTCGCCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACt                                                                                      >  2:1438257/1‑140 (MQ=255)                                              cTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACt                                                                                      <  1:759814/140‑1 (MQ=255)                                              cTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACt                                                                                      >  2:495937/1‑140 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     <  1:348402/140‑1 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     >  2:54286/1‑140 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     >  1:96520/1‑140 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     <  2:517843/140‑1 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     >  1:1047881/1‑140 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     >  2:516460/1‑140 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     <  1:395949/140‑1 (MQ=255)                                               tGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTa                                                                                     <  1:487178/140‑1 (MQ=255)                                                 tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAc                                                                                   >  1:672099/1‑140 (MQ=255)                                                 tCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAAc                                                                                   >  2:277145/1‑140 (MQ=255)                                                           aGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCa                                                                         >  2:1566188/1‑140 (MQ=255)                                                                 ccGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCt                                                                   >  1:1272012/1‑140 (MQ=255)                                                                 ccGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCt                                                                   >  1:361749/1‑140 (MQ=255)                                                                  cGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCtt                                                                  <  2:1530480/140‑1 (MQ=255)                                                                  cGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCtt                                                                  <  2:803116/140‑1 (MQ=255)                                                                   gATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTc                                                                 >  2:1221737/1‑140 (MQ=255)                                                                     tGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCaa                                                               >  2:570157/1‑140 (MQ=255)                                                                       cccGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAcc                                                             <  1:431820/140‑1 (MQ=255)                                                                        ccGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAAccc                                                            <  2:544895/140‑1 (MQ=255)                                                                         cgcgTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCg                                                           <  2:795955/140‑1 (MQ=255)                                                                          gcgTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCgg                                                          >  1:373049/1‑140 (MQ=255)                                                                             tGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGAcc                                                       <  2:96520/140‑1 (MQ=255)                                                                               gAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCATTATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCg                                                     >  1:657956/1‑140 (MQ=255)                                                                                  gCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTcc                                                  >  2:913158/1‑140 (MQ=255)                                                                                   ccATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTccc                                                 <  1:292404/140‑1 (MQ=255)                                                                                    cATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCt                                                >  1:1500019/1‑140 (MQ=255)                                                                                     aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtg                                               >  2:459831/1‑140 (MQ=255)                                                                                     aTCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGACCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtg                                               <  1:1444693/140‑1 (MQ=255)                                                                                      tCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtgt                                              >  2:808464/1‑140 (MQ=255)                                                                                      tCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtgt                                              >  2:341172/1‑140 (MQ=255)                                                                                      tCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCtgg                                              >  2:1121968/1‑139 (MQ=255)                                                                                               aGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAgg                                     <  1:48344/140‑1 (MQ=255)                                                                                                gCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAggg                                    <  1:277145/140‑1 (MQ=255)                                                                                                 cTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGc                                   >  2:1033834/1‑140 (MQ=255)                                                                                                  tGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCg                                  <  1:808464/140‑1 (MQ=255)                                                                                                  tGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCg                                  <  2:1481356/140‑1 (MQ=255)                                                                                                    aTTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                <  1:171547/140‑1 (MQ=255)                                                                                                    aTTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                <  2:843396/140‑1 (MQ=255)                                                                                                    aTTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                <  2:275361/140‑1 (MQ=255)                                                                                                    aTTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                <  1:470950/140‑1 (MQ=255)                                                                                                    aTTAAGCACCGCGCGGCGCAGGGCCAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAg                                <  1:660992/140‑1 (MQ=255)                                                                                                        aGCACCGCGCGGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGact                            <  1:1259357/140‑1 (MQ=255)                                                                                                              gcgcgGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACa                      >  2:991969/1‑140 (MQ=255)                                                                                                               cgcgGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACac                     >  1:867948/1‑139 (MQ=255)                                                                                                               cgcgGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACAt                     >  2:804021/1‑140 (MQ=255)                                                                                                               cgcgGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACAt                     >  1:286588/1‑140 (MQ=255)                                                                                                               cgcgGCGCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACAt                     >  2:124355/1‑140 (MQ=255)                                                                                                                  ggcgCAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGc                  >  2:1550786/1‑140 (MQ=255)                                                                                                                    cgcAGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCa                >  1:1288036/1‑140 (MQ=255)                                                                                                                       aGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCt             <  2:1047881/140‑1 (MQ=255)                                                                                                                       aGGGCGAGGCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCt             <  2:1272927/140‑1 (MQ=255)                                                                                                                              ggCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCtt      >  1:758048/1‑140 (MQ=255)                                                                                                                              ggCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCtt      >  2:1234816/1‑140 (MQ=255)                                                                                                                              ggCCGTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCtt      >  1:1575777/1‑140 (MQ=255)                                                                                                                                  gTTTCCGGCGCGGCGACAGCGGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGTCAGGGCGAGGACTACTACATCGCCCCCTCGACCTTCGAg  <  2:867948/140‑1 (MQ=255)                                                                                                                                  gTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAg  <  1:1486549/140‑1 (MQ=255)                                                                                                                                  gTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAg  <  1:991969/140‑1 (MQ=255)                                                                                                                                  gTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAg  <  1:516460/140‑1 (MQ=255)                                                                                                                                  gTTTCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAg  <  2:94044/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TTCTGCGTGGTGCCGGTGGTGCTCTACGCGGGCATGTTCATCATGCTGTCGCTCTACAAGCTCACCGATGCCCGCGTGGAGGCCATCAGCCGGCAGCTGATTAAGCACCGCGCGGCGCAGGGCGAGGCCGTTCCCGACGCCGCGACAGCCGCATCCCATTAACCGGAGGCAATATGGAAATCACTAACCCGATACTCACCGGCTTCAACCCGGACCCGTCCCTGTGCCGCCAGGGCGAGGACTACTACATCGCCACCTCGACCTTCGAG  >  NC_000913/286616‑286884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑