Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	298,590	A→G	L46P (CTG→CCG)	paoD ←	molybdenum cofactor insertion chaperone for PaoABC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,590	0	A	G	100.0%	253.3 / NA	68	L46P (CTG→CCG)	paoD	molybdenum cofactor insertion chaperone for PaoABC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/36);  total (32/36)

AGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGAC  >  NC_000913/298453‑298724                                                                                                                                          |                                                                                                                                       aGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGc                                                                                                                                      >  1:526716/1‑140 (MQ=255)      atcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCAc                                                                                                                                 <  1:274331/140‑1 (MQ=255)      atcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCAc                                                                                                                                 <  1:484909/140‑1 (MQ=255)       tcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCAcc                                                                                                                                >  1:1395144/1‑140 (MQ=255)       tcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCAcc                                                                                                                                >  1:494540/1‑140 (MQ=255)       tcatcTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCAcc                                                                                                                                >  1:113983/1‑140 (MQ=255)           ctcCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCa                                                                                                                            >  1:510434/1‑140 (MQ=255)             ccAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCACACCACCATCTGCGCCCCGAGCGGGCGCGCCGCACCGCCGCGTATCTCCACCGGCGTCACCAGCAcc                                                                                                                          <  2:259213/140‑1 (MQ=255)                gcgcTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCt                                                                                                                       >  1:725856/1‑140 (MQ=255)                gcgcTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCt                                                                                                                       >  2:678114/1‑140 (MQ=255)                    ttCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCtt                                                                                                                   >  2:1301554/1‑140 (MQ=255)                          agcggcagcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCgg                                                                                                             <  1:1116133/140‑1 (MQ=255)                                 gcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAg                                                                                                      <  1:1254937/140‑1 (MQ=255)                                 gcggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAg                                                                                                      <  2:773700/140‑1 (MQ=255)                                  cggcCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGACACCAGCACCGCTCCTTTACCGGACATTAgc                                                                                                     >  2:630423/1‑140 (MQ=255)                                           cGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACg                                                                                            >  1:480433/1‑140 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCCCCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  2:751932/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  2:1395144/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  2:494540/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  1:1451178/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  2:295381/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  1:613886/140‑1 (MQ=255)                                              aGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCg                                                                                         <  1:696063/140‑1 (MQ=255)                                                ccgccAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGaa                                                                                       >  1:209271/1‑140 (MQ=255)                                                     agagACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCa                                                                                  <  1:372928/140‑1 (MQ=255)                                                     agagACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCa                                                                                  <  1:127378/140‑1 (MQ=255)                                                        gaCAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGaa                                                                               <  2:180156/140‑1 (MQ=255)                                                        gaCAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGaa                                                                               <  1:270683/140‑1 (MQ=255)                                                        gaCAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGaa                                                                               <  1:853736/140‑1 (MQ=255)                                                        gaCAAAACCGCAGTAACCACCATCTTCGCGCACCACCATCTGCGCCCCGGGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGCCATTAGCGCCTCTACGGCGAAGCGCAGaa                                                                               <  2:597065/140‑1 (MQ=255)                                                            aaaCCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGt                                                                           >  2:707225/1‑140 (MQ=255)                                                            aaaCCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGt                                                                           <  1:956464/140‑1 (MQ=255)                                                                  cAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCggtg                                                                     >  1:82828/1‑140 (MQ=255)                                                                    gTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCggtggt                                                                   <  1:802226/140‑1 (MQ=255)                                                                             aTCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTga                                                          >  2:251223/1‑140 (MQ=255)                                                                                    cgcACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGc                                                   <  2:526716/140‑1 (MQ=255)                                                                                         cgccaTCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGct                                              <  1:1432067/138‑1 (MQ=255)                                                                                          accaTCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGctc                                             <  1:678114/140‑1 (MQ=255)                                                                                              tCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGc                                         >  2:22234/1‑140 (MQ=255)                                                                                              tCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGc                                         >  2:186093/1‑140 (MQ=255)                                                                                                 gcgcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTa                                      >  2:651594/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTCCCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGCCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  2:761544/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  1:654498/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  1:1162116/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  2:451467/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  1:527922/1‑140 (MQ=255)                                                                                                   gcCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATg                                    >  1:1484916/1‑140 (MQ=255)                                                                                                    ccccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                   <  1:707225/140‑1 (MQ=255)                                                                                                    ccccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                   >  2:1571788/1‑140 (MQ=255)                                                                                                    ccccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                   <  2:82828/140‑1 (MQ=255)                                                                                                    ccccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGc                                   <  1:595450/140‑1 (MQ=255)                                                                                                     cccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGcc                                  >  1:432181/1‑140 (MQ=255)                                                                                                     cccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGcc                                  >  1:1254850/1‑140 (MQ=255)                                                                                                     cccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCCCAGAATGGTCCGGCGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGcc                                  >  1:1042759/1‑140 (MQ=255)                                                                                                     cccGAGCGGACGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGcc                                  >  1:288513/1‑140 (MQ=255)                                                                                                      ccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCa                                 >  2:841045/1‑140 (MQ=255)                                                                                                      ccGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCa                                 <  2:1017209/140‑1 (MQ=255)                                                                                                        gAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgt                               >  2:985911/1‑140 (MQ=255)                                                                                                         aGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgtg                              >  2:376529/1‑140 (MQ=255)                                                                                                         aGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgtg                              >  2:1543206/1‑140 (MQ=255)                                                                                                         aGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgtg                              >  1:1477376/1‑140 (MQ=255)                                                                                                          gCGGGCGCGCCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAgtgt                             <  1:186093/140‑1 (MQ=255)                                                                                                              gcgcgcCGCGCCGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGt                         <  1:1382913/140‑1 (MQ=255)                                                                                                                gcgccgcgccGCCGCGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCt                       <  2:152847/140‑1 (MQ=255)                                                                                                                          gccgcGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCaaaaa             <  2:666548/140‑1 (MQ=255)                                                                                                                           ccgcGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAg            <  2:113983/140‑1 (MQ=255)                                                                                                                           ccgcGTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACA‑TAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGc           <  2:725856/140‑1 (MQ=255)                                                                                                                            cgcgTATCTCCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGc           <  2:1433826/140‑1 (MQ=255)                                                                                                                                    tcCACCGGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGa   <  1:76312/140‑1 (MQ=255)                                                                                                                                     ccaccgGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGAc  >  1:718906/1‑140 (MQ=255)                                                                                                                                     ccaccgGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGAc  >  1:645637/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AGCCCATCATCTCCAGCGCTTCAAAAGCGGCAGCGGCCTCCACGCAGCCGCCAGAGACAAAACCGCAGTAACGACCATCTTCGCGCACCACCATCTGCGCCCCGAGCGGGCGCGCCGCGCCGCCGCGTATCTCCACCAGCGTCACCAGCACCGCTCCTTTACCGGACATTAGCGCCTCTACGGCGAAGCGCAGAATGGTCCGGTGGTCATCGGTGAGAAACGCCTGCTCTGGCTTATGCCAGTGTTCGTCTTTGTCAAAAAGCGGGTATGAC  >  NC_000913/298453‑298724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑