Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	846,384	C→T	G27D (GGC→GAC)	glnQ ←	L‑glutamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	846,384	0	C	T	100.0%	265.6 / NA	78	G27D (GGC→GAC)	glnQ	L‑glutamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/44);  total (34/44)

TCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGCCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCA  >  NC_000913/846249‑846512                                                                                                                                        |                                                                                                                                 tCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTg                                                                                                                              >  2:764146/1‑140 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  2:1343410/140‑1 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  2:754324/140‑1 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  2:405327/140‑1 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  1:1163355/140‑1 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  2:43520/140‑1 (MQ=255)          ttCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGtt                                                                                                                     <  1:1570522/140‑1 (MQ=255)            cGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCa                                                                                                                   <  1:571201/140‑1 (MQ=255)               aTCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAAt                                                                                                                <  1:686751/140‑1 (MQ=255)                         ttCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATtgtg                                                                                                      <  2:450181/140‑1 (MQ=255)                          tCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgc                                                                                                     <  1:880323/140‑1 (MQ=255)                          tCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgc                                                                                                     <  1:1043628/140‑1 (MQ=255)                          tCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgc                                                                                                     <  1:1127141/140‑1 (MQ=255)                          tCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgc                                                                                                     <  2:717401/140‑1 (MQ=255)                          tCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgc                                                                                                     <  2:70106/140‑1 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    <  1:764146/140‑1 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    >  1:734586/1‑140 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    >  2:579358/1‑140 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    >  1:437253/1‑140 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    >  1:378423/1‑140 (MQ=255)                           cAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgca                                                                                                    >  1:1161/1‑140 (MQ=255)                            aGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgcag                                                                                                   >  1:1574566/1‑140 (MQ=255)                              gCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTgcagca                                                                                                 >  1:258249/1‑140 (MQ=255)                               ccATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCAc                                                                                                <  1:579358/140‑1 (MQ=255)                                 aTCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCt                                                                                              >  1:1337767/1‑140 (MQ=255)                                  tCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTg                                                                                             >  2:861822/1‑140 (MQ=255)                                  tCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTg                                                                                             >  2:936709/1‑140 (MQ=255)                                  tCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTg                                                                                             >  1:109359/1‑140 (MQ=255)                                   cGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTgg                                                                                            >  2:258347/1‑140 (MQ=255)                                    gACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCTCCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTggg                                                                                           <  1:1285839/140‑1 (MQ=255)                                        aTCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTgg                                                                                       >  2:1256621/1‑140 (MQ=255)                                         tCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTggg                                                                                      <  2:1412021/140‑1 (MQ=255)                                            gATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCa                                                                                   <  2:1490367/140‑1 (MQ=255)                                            gATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATACAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCGCGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCa                                                                                   <  1:492404/140‑1 (MQ=255)                                             aTCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCaa                                                                                  >  1:311914/1‑140 (MQ=255)                                             aTCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCaa                                                                                  <  2:896987/140‑1 (MQ=255)                                             aTCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCaa                                                                                  >  1:1080058/1‑140 (MQ=255)                                              tCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGACTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCaaa                                                                                 >  2:962606/1‑140 (MQ=255)                                               cGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAg                                                                                >  1:432970/1‑140 (MQ=255)                                               cGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAg                                                                                <  2:541367/140‑1 (MQ=255)                                                    gAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCtt                                                                           >  1:1166238/1‑140 (MQ=255)                                                           tttCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACg                                                                    <  2:1253229/140‑1 (MQ=255)                                                                 ccAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTa                                                              <  2:1210342/140‑1 (MQ=255)                                                                       tgttgATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCa                                                        >  1:1572037/1‑140 (MQ=255)                                                                            aTGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATcac                                                   <  2:378423/140‑1 (MQ=255)                                                                             tGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATcaca                                                  <  1:936709/140‑1 (MQ=255)                                                                                  cgcagcAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGAtt                                             >  1:848821/1‑140 (MQ=255)                                                                                      gcagGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCa                                         <  2:1574566/140‑1 (MQ=255)                                                                                          ggTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCct                                     >  1:1222986/1‑140 (MQ=255)                                                                                          ggTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCct                                     <  2:1080058/140‑1 (MQ=255)                                                                                          ggTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCct                                     <  2:1103752/140‑1 (MQ=255)                                                                                          ggTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCct                                     <  1:65624/140‑1 (MQ=255)                                                                                            tCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCcttc                                   >  2:1035895/1‑140 (MQ=255)                                                                                            tCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCcttc                                   >  1:1520292/1‑140 (MQ=255)                                                                                            tCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCcttc                                   >  1:281303/1‑140 (MQ=255)                                                                                            tCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCcttc                                   >  1:936189/1‑140 (MQ=255)                                                                                               aTTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCttt                                >  2:1380276/1‑140 (MQ=255)                                                                                                    ccggaaccggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAga                           <  2:243654/140‑1 (MQ=255)                                                                                                    ccggaaccggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAga                           <  1:962606/140‑1 (MQ=255)                                                                                                    ccggaaccggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAga                           <  2:432970/140‑1 (MQ=255)                                                                                                     cggaaccggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAgac                          >  1:1399608/1‑140 (MQ=255)                                                                                                        aaccggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAgacgac                       >  1:526735/1‑140 (MQ=255)                                                                                                         accggaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGacgacg                      <  1:264984/140‑1 (MQ=255)                                                                                                             gaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGa                  >  1:957027/1‑140 (MQ=255)                                                                                                             gaCGGCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGa                  >  1:439147/1‑140 (MQ=255)                                                                                                                ggCCCGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATa               <  2:1237601/140‑1 (MQ=255)                                                                                                                  cccGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATaaa             >  2:1371393/1‑140 (MQ=255)                                                                                                                  cccGATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATaaa             >  1:663716/1‑140 (MQ=255)                                                                                                                     gATAATCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCt          <  2:1399608/140‑1 (MQ=255)                                                                                                                          tCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCa     <  2:437253/140‑1 (MQ=255)                                                                                                                          tCACCACGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCa     <  2:109359/140‑1 (MQ=255)                                                                                                                            accacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCAcc   >  2:540009/1‑140 (MQ=255)                                                                                                                            accacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCAcc   <  1:861822/140‑1 (MQ=255)                                                                                                                             ccacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCa  <  2:1337767/140‑1 (MQ=255)                                                                                                                             ccacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCa  <  2:1572037/140‑1 (MQ=255)                                                                                                                             ccacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCa  <  2:936189/140‑1 (MQ=255)                                                                                                                             ccacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCa  <  2:570044/140‑1 (MQ=255)                                                                                                                             ccacGACTTCGTCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCa  <  2:1520292/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 TCGTCAACTTTCGGATCGTTAACCTTCAGGCCATCGACAATCAGATCGCCGGAGGTGATTTCTTCCAGTTTGTTGATGCAGCGCAGCAGGGTCGATTTACCGGAACCGGACGGCCCGATAATCACCACGACTTCGCCCTGGGCAATGTTCAAATCGATATTGTGCAGCACCTGGGTTGGGCCAAAGTGCTTGGAGACGTTTTTAAATTCAATCACAGGATTTTCATCCTTCTTTCCAGACGACGCAGAATAAAGCTCAGCACCA  >  NC_000913/846249‑846512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑