Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,319,424	T→C	Y122C (TAC→TGC)	trpC ←	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,319,424	0	T	C	100.0%	236.5 / NA	62	Y122C (TAC→TGC)	trpC	fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

ATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGTAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAA  >  NC_000913/1319288‑1319558                                                                                                                                         |                                                                                                                                       aTTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAg                                                                                                                                     >  1:1246152/1‑140 (MQ=255)    aCTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGt                                                                                                                                  <  2:994954/140‑1 (MQ=255)      tGACTTCGGTCAGCCCCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCg                                                                                                                                <  1:1145142/140‑1 (MQ=255)      tGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCg                                                                                                                                <  1:1051745/140‑1 (MQ=255)          ttCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGTGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATaa                                                                                                                            >  1:347429/1‑140 (MQ=255)          ttCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATaa                                                                                                                            >  1:582291/1‑140 (MQ=255)            cGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATg                                                                                                                          <  2:611978/140‑1 (MQ=255)             ggTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGa                                                                                                                         >  1:1136275/1‑140 (MQ=255)             ggTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGa                                                                                                                         <  2:683135/140‑1 (MQ=255)                  gCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCt                                                                                                                    <  1:818665/140‑1 (MQ=255)                  gCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCt                                                                                                                    >  2:588427/1‑140 (MQ=255)                      ccccATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTAc                                                                                                                >  1:1525247/1‑140 (MQ=255)                                aGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCgg                                                                                                      >  1:1087975/1‑140 (MQ=255)                                  aCTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCt                                                                                                    >  1:363425/1‑140 (MQ=255)                                   cTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTg                                                                                                   <  2:1579694/140‑1 (MQ=255)                                      tgAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCgg                                                                                                >  1:1086962/1‑140 (MQ=255)                                      tgAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCgg                                                                                                >  2:1510497/1‑140 (MQ=255)                                      tgAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGCCTTTACATAAAATCGGCTGCgg                                                                                                >  1:1150270/1‑140 (MQ=255)                                      tgAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGAAATAGCACGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCgg                                                                                                >  2:1426453/1‑140 (MQ=255)                                       gAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCggg                                                                                               >  1:1441397/1‑140 (MQ=255)                                       gAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCggg                                                                                               >  2:1343059/1‑140 (MQ=255)                                       gAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCggg                                                                                               <  1:1369547/140‑1 (MQ=255)                                        aGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGTGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCgggg                                                                                              <  1:1284874/140‑1 (MQ=255)                                         gCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGc                                                                                             >  1:1418749/1‑140 (MQ=255)                                             cggcggCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGAtt                                                                                         >  2:1104499/1‑140 (MQ=255)                                                cggcAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTgg                                                                                      >  2:106913/1‑140 (MQ=255)                                                  gcAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCt                                                                                    <  2:1439136/140‑1 (MQ=255)                                                   cAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTg                                                                                   <  1:1098893/140‑1 (MQ=255)                                                          gCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATgg                                                                            >  1:852820/1‑140 (MQ=255)                                                                ttGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGggagga                                                                      <  1:656114/140‑1 (MQ=255)                                                                       tcATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTaaa                                                               <  2:1246152/140‑1 (MQ=255)                                                                         aTCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGc                                                             >  1:12259/1‑140 (MQ=255)                                                                               tACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCt                                                       <  1:1104499/140‑1 (MQ=255)                                                                                 cTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGa                                                     <  1:588427/140‑1 (MQ=255)                                                                                    aaaGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGaaaa                                                  >  1:1361658/1‑140 (MQ=255)                                                                                    aaaGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGaaaa                                                  >  1:1384368/1‑140 (MQ=255)                                                                                      aGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAata                                                <  2:661363/140‑1 (MQ=255)                                                                                         aTTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATAttt                                             >  1:594710/1‑140 (MQ=255)                                                                                         aTTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATAttt                                             >  1:176805/1‑140 (MQ=255)                                                                                                aGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCtcatca                                      >  2:13958/1‑140 (MQ=255)                                                                                                  cAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCAtcagt                                    >  1:128790/1‑140 (MQ=255)                                                                                                               ggTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAAtt                       >  1:1461347/1‑140 (MQ=255)                                                                                                               ggTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAAtt                       >  2:1540510/1‑140 (MQ=255)                                                                                                               ggTAATAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGCAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAAtt                       >  2:261929/1‑140 (MQ=255)                                                                                                                   aTAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCg                   <  1:106913/140‑1 (MQ=255)                                                                                                                   aTAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCg                   <  2:342921/140‑1 (MQ=255)                                                                                                                    tAGCGCGCCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGGCGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGa                  <  2:1150270/140‑1 (MQ=255)                                                                                                                         cgcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGt             >  2:797121/1‑140 (MQ=255)                                                                                                                         cgcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGt             <  2:1525247/140‑1 (MQ=255)                                                                                                                         cgcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGt             >  1:925905/1‑140 (MQ=255)                                                                                                                         cgcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGt             <  2:363425/140‑1 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            <  2:347429/140‑1 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            <  2:1062774/140‑1 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            >  1:1097149/1‑140 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            <  2:1086962/140‑1 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            >  2:1399628/1‑140 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            <  2:1087975/140‑1 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            >  1:485404/1‑140 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTa            >  1:281105/1‑140 (MQ=255)                                                                                                                          gcCAGATAGATCTGGCAAGGGTCGATAATGAAGTCTCTGCATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCCCCGAAATTGCCGAAGCGTa            <  2:924688/140‑1 (MQ=255)                                                                                                                           ccAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCGGTCAGCACCGAAATTGCCGAAGCGTaa           >  1:1325018/1‑140 (MQ=255)                                                                                                                           ccAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTaa           <  1:1510497/140‑1 (MQ=255)                                                                                                                           ccAGATAGATCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTaa           >  1:1555553/1‑140 (MQ=255)                                                                                                                                    tCTGGCAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATaa  >  1:1454194/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ATTACTGACTTCGGTCAGCACCCCCATCTCCAGACTGTGAGCGACGGCGGCAAGCTGGCGATATTGGTCGTCATCCAGTACTGAAAGCATTAATAAGCAGGCATCGGCCTGGTAATAGCGCGCCAGATAGATCTGGTAAGGGTCGATAATGAAGTCTTTACATAAAATCGGCTGCGGGGCGATTTGGCTGACGATGGGGAGGAAATTAAAGCTCCCCTGAAAATATTTCTCATCAGTCAGCACCGAAATTGCCGAAGCGTAATGTTTATAA  >  NC_000913/1319288‑1319558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑