Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,645	T→C	V240A (GTT→GCT)	paaA →	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,645	0	T	C	100.0%	244.9 / NA	64	V240A (GTT→GCT)	paaA	phenylacetyl‑CoA 1,2‑epoxidase, monooxygenase subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/24);  total (40/24)

GTTCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGTTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCC  >  NC_000913/1454508‑1454781                                                                                                                                          |                                                                                                                                         gTTCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTc                                                                                                                                        >  2:909636/1‑140 (MQ=255) gTTCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTc                                                                                                                                        >  2:1439479/1‑140 (MQ=255) gTTCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGGTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTc                                                                                                                                        >  1:1546234/1‑140 (MQ=255)  ttCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTcc                                                                                                                                       >  2:555106/1‑140 (MQ=255)    cGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTg                                                                                                                                     >  2:979088/1‑140 (MQ=255)     gggCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGa                                                                                                                                    >  1:33214/1‑140 (MQ=255)     gggCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGATATGACCGCTCCTGa                                                                                                                                    >  2:298916/1‑140 (MQ=255)       gCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGAcc                                                                                                                                  >  1:795119/1‑140 (MQ=255)             cGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATc                                                                                                                            <  2:458254/140‑1 (MQ=255)               atgatAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTg                                                                                                                          >  2:1007749/1‑140 (MQ=255)                      cTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTg                                                                                                                   >  1:14388/1‑140 (MQ=255)                        cGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGac                                                                                                                 >  1:171823/1‑140 (MQ=255)                        cGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGac                                                                                                                 <  1:979088/140‑1 (MQ=255)                         gCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGaca                                                                                                                >  2:895427/1‑140 (MQ=255)                         gCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGaca                                                                                                                >  2:1352187/1‑140 (MQ=255)                          ccAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGacac                                                                                                               >  1:772478/1‑140 (MQ=255)                                 gcgcCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGc                                                                                                        >  2:78288/1‑140 (MQ=255)                                         aGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTa                                                                                                <  1:1057148/140‑1 (MQ=255)                                             tcACCTGGAAAATCAAACGTTTCACCAACGCCGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGc                                                                                            >  1:242781/1‑140 (MQ=255)                                               aCCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCtt                                                                                          >  1:340322/1‑140 (MQ=255)                                                  tGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTgg                                                                                       <  1:499/140‑1 (MQ=255)                                                    gAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTg                                                                                     >  2:1356060/1‑140 (MQ=255)                                                         tCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGGGAGATc                                                                                >  2:1147037/1‑140 (MQ=255)                                                             aCGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACt                                                                            <  1:1388921/140‑1 (MQ=255)                                                                tttCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGc                                                                         <  1:909636/140‑1 (MQ=255)                                                                 ttCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCa                                                                        <  1:555106/140‑1 (MQ=255)                                                                 ttCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCa                                                                        <  2:795119/140‑1 (MQ=255)                                                                           acgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTaat                                                              >  2:1502712/1‑139 (MQ=255)                                                                           acgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAAc                                                              >  1:506218/1‑140 (MQ=255)                                                                           acgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAAc                                                              >  1:1264009/1‑140 (MQ=255)                                                                           acgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAAc                                                              >  1:1451068/1‑140 (MQ=255)                                                                           acgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAAc                                                              >  1:1347702/1‑140 (MQ=255)                                                                            cgaGCTCCGCCAGCGTTTCGTGGATAACACCGTTCCCCAGGTTGAAATGCTCGGTATGACCGCTCCTGGCCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACg                                                             <  2:573476/140‑1 (MQ=255)                                                                            cgaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACg                                                             <  2:105416/140‑1 (MQ=255)                                                                             gaACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGa                                                            <  2:487100/140‑1 (MQ=255)                                                                               aCTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAg                                                          <  1:1439479/140‑1 (MQ=255)                                                                                   cGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAAt                                                      >  1:415820/1‑140 (MQ=255)                                                                                    gCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAAtt                                                     <  1:994247/140‑1 (MQ=255)                                                                                    gCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAAtt                                                     >  1:123216/1‑140 (MQ=255)                                                                                    gCCAGCGTTCCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAAtt                                                     >  2:1126559/1‑140 (MQ=255)                                                                                             tCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTcgc                                            >  2:651521/1‑140 (MQ=255)                                                                                               gTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCgg                                          <  2:242781/140‑1 (MQ=255)                                                                                                  gATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAAt                                       <  1:1502712/140‑1 (MQ=255)                                                                                                  gATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAAt                                       <  1:1303457/140‑1 (MQ=255)                                                                                                  gATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAAt                                       <  2:624510/140‑1 (MQ=255)                                                                                                    tAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAAttt                                     <  2:14388/140‑1 (MQ=255)                                                                                                     aaCACCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTg                                    <  2:1347702/140‑1 (MQ=255)                                                                                                       cacCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTa                                  >  1:1000184/1‑140 (MQ=255)                                                                                                       cacCGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTa                                  >  2:1335468/1‑140 (MQ=255)                                                                                                         ccGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAAt                                <  2:33214/140‑1 (MQ=255)                                                                                                          cGTTCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATc                               >  1:824670/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  1:656663/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  2:298007/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  2:898178/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  2:521000/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  1:1233152/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  2:1093186/1‑140 (MQ=255)                                                                                                            ttCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAg                             >  1:328619/1‑140 (MQ=255)                                                                                                             tCCACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAgg                            >  2:1344728/1‑140 (MQ=255)                                                                                                              ccACAGGTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGga                           >  1:344522/1‑140 (MQ=255)                                                                                                                    gTTGAAATGCTCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTCTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCt                     <  2:772478/140‑1 (MQ=255)                                                                                                                              tCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCaaa           >  2:152005/1‑140 (MQ=255)                                                                                                                              tCGGTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCaaa           >  2:15662/1‑140 (MQ=255)                                                                                                                                 gTATGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGt        <  2:1264009/140‑1 (MQ=255)                                                                                                                                   aTGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTaa      <  2:1269291/140‑1 (MQ=255)                                                                                                                                    tGACCGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTaaa     <  1:298916/140‑1 (MQ=255)                                                                                                                                       ccGCTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGcc  <  2:1000184/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTTCGGGCCAAACGATGATAACTCGCCAAACAGCGCCAGAAGTCTCACCTGGAAAATCAAACGTTTCACCAACGACGAACTCCGCCAGCGTTTCGTGGATAACACCGTTCCACAGGTTGAAATGCTCGGTATGACCGTTCCTGACCCGGATCTGCATTTTGACACTGAAAGCGGTCACTACCGCTTTGGTGAGATCGACTGGCAGGAGTTTAACGAAGTAATTAACGGTCGCGGAATTTGTAATCAGGAGAGGCTCGACGCCAAACGTAAAGCC  >  NC_000913/1454508‑1454781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑