Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,522,527	C→T	G180E (GGG→GAG)	yncD ←	putative TonB‑dependent outer membrane receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,522,527	0	C	T	100.0%	268.1 / NA	78	G180E (GGG→GAG)	yncD	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/46);  total (32/46)

TTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAG  >  NC_000913/1522394‑1522660                                                                                                                                      |                                                                                                                                      tttGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCCTTGCTTTCAGCTCATAgc                                                                                                                                 >  2:935890/1‑140 (MQ=255)       aTTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGc                                                                                                                           >  1:1128667/1‑140 (MQ=255)         tGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTg                                                                                                                         >  2:946746/1‑140 (MQ=255)           gCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGcc                                                                                                                       >  1:1587313/1‑140 (MQ=255)           gCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGcc                                                                                                                       >  1:1095560/1‑140 (MQ=255)           gCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGcc                                                                                                                       >  2:847443/1‑140 (MQ=255)           gCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGcc                                                                                                                       >  2:572181/1‑140 (MQ=255)               aaTTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCaaaa                                                                                                                   >  1:867529/1‑140 (MQ=255)                aTTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCATGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAAc                                                                                                                  >  1:172460/1‑140 (MQ=255)                  ttttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTg                                                                                                                <  2:504812/140‑1 (MQ=255)                  ttttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTg                                                                                                                <  1:341209/140‑1 (MQ=255)                  ttttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTg                                                                                                                <  2:757196/140‑1 (MQ=255)                    ttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGcc                                                                                                              <  1:1298179/140‑1 (MQ=255)                    ttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGcc                                                                                                              <  2:743624/140‑1 (MQ=255)                    ttCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGcc                                                                                                              <  1:1394687/140‑1 (MQ=255)                       tgtgCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCgta                                                                                                           <  1:808550/140‑1 (MQ=255)                        gtgCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCgtag                                                                                                          <  1:582405/140‑1 (MQ=255)                           cgcCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTaa                                                                                                       <  2:809394/140‑1 (MQ=255)                                cTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAactact                                                                                                  <  1:1069175/140‑1 (MQ=255)                                 tATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTg                                                                                                 <  2:296165/140‑1 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          <  1:354966/140‑1 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          >  1:1490591/1‑140 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          <  1:1108555/140‑1 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          <  2:1128667/140‑1 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          >  2:61332/1‑140 (MQ=255)                                        aCGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCaa                                                                                          <  1:136730/140‑1 (MQ=255)                                         cGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAAt                                                                                         <  1:577188/140‑1 (MQ=255)                                             aGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAAtggtt                                                                                     >  1:206471/1‑140 (MQ=255)                                             aGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAAtggtt                                                                                     <  2:1587919/140‑1 (MQ=255)                                               ccGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAAtggttgg                                                                                   >  2:1305294/1‑140 (MQ=255)                                                    ggTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCt                                                                              >  2:594607/1‑140 (MQ=255)                                                    ggTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCt                                                                              >  1:290643/1‑140 (MQ=255)                                                    ggTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCt                                                                              <  1:1116040/140‑1 (MQ=255)                                                        gTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCtgttg                                                                          >  1:170121/1‑140 (MQ=255)                                                        gTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCtgttg                                                                          >  1:903032/1‑140 (MQ=255)                                                              cgcgTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGt                                                                    <  1:736203/140‑1 (MQ=255)                                                              cgcgTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGt                                                                    <  2:1062778/140‑1 (MQ=255)                                                              cgcgTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGt                                                                    <  1:1134329/140‑1 (MQ=255)                                                                      tGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGggtgg                                                            <  2:459323/140‑1 (MQ=255)                                                                        agaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGgtggtg                                                          <  2:950611/140‑1 (MQ=255)                                                                         gaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGa                                                         <  2:1490591/140‑1 (MQ=255)                                                                         gaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGa                                                         <  2:1587313/140‑1 (MQ=255)                                                                         gaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGa                                                         <  1:457042/140‑1 (MQ=255)                                                                         gaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGa                                                         <  2:903032/140‑1 (MQ=255)                                                                         gaCGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGa                                                         <  2:206471/140‑1 (MQ=255)                                                                           cGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAca                                                       <  2:28394/140‑1 (MQ=255)                                                                           cGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAca                                                       <  1:446400/140‑1 (MQ=255)                                                                             gtgtAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAcatt                                                     >  1:946016/1‑140 (MQ=255)                                                                             gtgtAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAcatt                                                     >  1:1311373/1‑140 (MQ=255)                                                                              tgtAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAcattc                                                    >  2:1556006/1‑140 (MQ=255)                                                                              tgtAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAcattc                                                    >  1:980837/1‑140 (MQ=255)                                                                                tAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGAcattcat                                                  >  2:516125/1‑140 (MQ=255)                                                                                   tCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTAc                                               >  2:1111096/1‑140 (MQ=255)                                                                                        aTCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCccac                                          >  1:108629/1‑140 (MQ=255)                                                                                        aTCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGCAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCccac                                          >  2:299576/1‑140 (MQ=255)                                                                                          cGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATTGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCccacca                                        <  1:1555737/140‑1 (MQ=255)                                                                                          cGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCccacca                                        >  2:1243065/1‑140 (MQ=255)                                                                                            ccAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGa                                      >  2:1347164/1‑140 (MQ=255)                                                                                              gggCTGTGTGCCGTCTCCCGTTGCGCCCTTTGCTTTCAGCTTATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                    <  1:935890/139‑1 (MQ=255)                                                                                              gggCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                    <  1:996675/139‑1 (MQ=255)                                                                                              agccTGTGGGCCGCCTCCCGTGGCGACCGTTGCTTTCAGCTCAGAGCGCCACGTGCTAAAACTGCCGTAGTAACTAGTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGTCATTCATTTCCCCACCAGAcg                                    <  2:172460/137‑1 (MQ=255)                                                                                              aGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                    <  2:1131503/140‑1 (MQ=255)                                                                                              aGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                    <  2:867529/140‑1 (MQ=255)                                                                                              aGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcg                                    <  1:169126/140‑1 (MQ=255)                                                                                               ggCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGAcgc                                   <  1:61332/140‑1 (MQ=255)                                                                                                   gtgtgCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTg                               <  2:646993/140‑1 (MQ=255)                                                                                                    tgtgCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCCGGGTGGTGACATTCATTACCCCACCAGACGCGTTGc                              >  1:1315385/1‑140 (MQ=255)                                                                                                        ccGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATa                          <  2:22187/140‑1 (MQ=255)                                                                                                        ccGTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATa                          <  1:191274/140‑1 (MQ=255)                                                                                                          gTCTCCCGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACa                        <  1:572181/140‑1 (MQ=255)                                                                                                              cccGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGc                    <  2:227398/140‑1 (MQ=255)                                                                                                               ccGTTGCGCCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCa                   <  1:164254/140‑1 (MQ=255)                                                                                                                     cgcCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAg             >  1:1104379/1‑140 (MQ=255)                                                                                                                      gcCCGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAgg            >  1:1273444/1‑140 (MQ=255)                                                                                                                       cccGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAggg           >  2:534083/1‑140 (MQ=255)                                                                                                                        ccGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAgggg          <  1:1377395/140‑1 (MQ=255)                                                                                                                        ccGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAgggg          <  1:946746/140‑1 (MQ=255)                                                                                                                         cGTTGCTTTCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGc         >  1:1364122/1‑140 (MQ=255)                                                                                                                                ttCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACgcag  <  1:594607/140‑1 (MQ=255)                                                                                                                                ttCAGCTCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACgcag  <  1:1111096/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTTGGCATTGGCTAAATTTTTCTGTGCGCCACTATGGTCACGATAGCCGTGGGTCGTAAAACGCGTGGTTGAGACGGTGTAATCGACATCGCCAGGCTGTGTGCCGTCTCCCGTTGCGCCCGTTGCTTTCAGCCCATAGCGCCAGCTGCCAAAACTGCCGTAGTAACTACTGGCTTCAATGGTTGGTGGCTGTTGTCCGGTCTGGGTGGTGACATTCATTACCCCACCAGACGCGTTGCCATACAGGGCAGAGAAGGGGCCACGCAG  >  NC_000913/1522394‑1522660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑