BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	84,997	A→G	Q210Q (CAA→CAG)	leuO →	DNA‑binding transcriptional dual regulator LeuO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	84,997	0	A	G	100.0%	392.2 / NA	103	Q210Q (CAA→CAG)	leuO	DNA‑binding transcriptional dual regulator LeuO
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/59); total (44/59)

Only 100 of 105 total aligned reads displayed.

TTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTG  >  NC_000913/84864‑85132

ttAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATgcg                                                                                                                                   <  1:717842/140‑1 (MQ=255)

   gTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATgcggcg                                                                                                                                >  1:435917/1‑140 (MQ=255)

    ttATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGcggcgg                                                                                                                               <  2:201871/140‑1 (MQ=255)

     tATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGt                                                                                                                              <  1:848757/140‑1 (MQ=255)

     tATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGt                                                                                                                              <  2:937688/140‑1 (MQ=255)

         aaGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCg                                                                                                                          >  2:1417104/1‑140 (MQ=255)

             cTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCg                                                                                                                      >  1:575857/1‑140 (MQ=255)

             cTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCg                                                                                                                      >  2:1029157/1‑140 (MQ=255)

              ttCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGa                                                                                                                     >  1:1581661/1‑140 (MQ=255)

              ttCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGa                                                                                                                     >  1:706053/1‑140 (MQ=255)

              ttCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGa                                                                                                                     >  2:882201/1‑140 (MQ=255)

              ttCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGa                                                                                                                     >  2:176906/1‑140 (MQ=255)

               tCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGAt                                                                                                                    <  1:988739/140‑1 (MQ=255)

                       ccTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTcgc                                                                                                            <  1:495163/140‑1 (MQ=255)

                        cTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTcgcg                                                                                                           <  1:427480/140‑1 (MQ=255)

                        cTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTcgcg                                                                                                           <  1:400627/140‑1 (MQ=255)

                           aaTTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCa                                                                                                        <  1:1018737/140‑1 (MQ=255)

                           aaTTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCa                                                                                                        <  1:1157721/140‑1 (MQ=255)

                              ttACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCAttt                                                                                                     <  1:1082580/140‑1 (MQ=255)

                                 ccAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGt                                                                                                  <  2:437090/140‑1 (MQ=255)

                                 ccAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGt                                                                                                  <  1:1119049/140‑1 (MQ=255)

                                 ccAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGAGGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGt                                                                                                  <  1:1296822/140‑1 (MQ=255)

                                     cGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc                                                                                              >  2:570281/1‑140 (MQ=255)

                                     cGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc                                                                                              <  1:1226880/140‑1 (MQ=255)

                                     cGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc                                                                                              >  2:1080075/1‑140 (MQ=255)

                                     cGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAc                                                                                              <  1:1536441/140‑1 (MQ=255)

                                      gTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAAcc                                                                                             <  1:89593/140‑1 (MQ=255)

                                       tACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCt                                                                                            >  2:412212/1‑140 (MQ=255)

                                        aCCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCtt                                                                                           >  2:845522/1‑140 (MQ=255)

                                          cATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTTCTGAATCATGATGTTTATAACGAACAGCATGCGGCGGTTTCTCACGATCGTTTCGCGTCATTTAGTCAACCTTgg                                                                                         <  2:32099/140‑1 (MQ=255)

                                          cATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTgg                                                                                         <  2:1033506/140‑1 (MQ=255)

                                            ttattTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTa                                                                                       <  2:1341248/140‑1 (MQ=255)

                                               tttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAATAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGa                                                                                    >  2:963055/1‑140 (MQ=255)

                                               tttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGa                                                                                    >  2:1584231/1‑140 (MQ=255)

                                               tttAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGa                                                                                    >  1:233982/1‑140 (MQ=255)

                                                 tAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGaca                                                                                  >  1:1308502/1‑140 (MQ=255)

                                                  aaaGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTTCTGAAGCATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGacac                                                                                 <  1:1074171/140‑1 (MQ=255)

                                                  aaaGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGacac                                                                                 <  2:351799/140‑1 (MQ=255)

                                                  aaaGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGacac                                                                                 <  1:543284/140‑1 (MQ=255)

                                                  aaaGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGacac                                                                                 <  1:882201/140‑1 (MQ=255)

                                                    aGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg                                                                               >  1:1166625/1‑140 (MQ=255)

                                                    aGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACgg                                                                               >  1:1459973/1‑140 (MQ=255)

                                                       tGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAg                                                                            <  2:1231307/140‑1 (MQ=255)

                                                       tGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAg                                                                            <  1:147322/140‑1 (MQ=255)

                                                       tGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGATTCGCTCGATCGTTTCGCGTCATTTAATCAAACTTGGTATGaaaccgaaa                                                                            >  1:1400536/1‑135 (MQ=255)

                                                          aaTGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATa                                                                         <  1:1451356/140‑1 (MQ=255)

                                                            tGGTGTTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaag                                                                       <  1:438815/140‑1 (MQ=255)

                                                            tGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaag                                                                       <  2:801423/140‑1 (MQ=255)

                                                            tGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaag                                                                       <  2:124845/140‑1 (MQ=255)

                                                              gTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagca                                                                     <  1:1029157/140‑1 (MQ=255)

                                                               tGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATaagcaa                                                                    >  1:252668/1‑140 (MQ=255)

                                                                   ggTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGGAGATAAGCAAGCCa                                                                >  1:302256/1‑140 (MQ=255)

                                                                    gTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAg                                                               <  2:432439/140‑1 (MQ=255)

                                                                    gTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAg                                                               <  2:976077/140‑1 (MQ=255)

                                                                      aGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATACCGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGAAACGGTAGATAAGCAAGCCAGTa                                                             <  1:1013044/140‑1 (MQ=255)

                                                                        ccAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGTTCGTTTCGCGTCATTTAGTATACCTTGGCATTCCACGGTAGATAAACAACGCAGtcac                                                           >  2:483137/1‑137 (MQ=255)

                                                                        ccAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGGCAACCTTGGTATGACACGGTAGATAAACCAGCCAGCATc                                                           >  1:1581272/1‑140 (MQ=255)

                                                                         cAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcg                                                          <  1:1547594/140‑1 (MQ=255)

                                                                         cAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcg                                                          <  1:1417104/140‑1 (MQ=255)

                                                                         cAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcg                                                          >  1:1283556/1‑140 (MQ=255)

                                                                          aGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcgc                                                         >  1:1337727/1‑140 (MQ=255)

                                                                           gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATcgcg                                                        >  2:758413/1‑140 (MQ=255)

                                                                            cAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCGAGCCAGTATCGCGt                                                       >  2:869547/1‑140 (MQ=255)

                                                                              aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTAt                                                     <  2:1284118/140‑1 (MQ=255)

                                                                              aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTAt                                                     <  1:23626/140‑1 (MQ=255)

                                                                              aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTAt                                                     <  1:176906/140‑1 (MQ=255)

                                                                                aaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCa                                                   <  1:1369538/140‑1 (MQ=255)

                                                                                   catcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAggg                                                <  1:1080075/140‑1 (MQ=255)

                                                                                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCa                                              <  1:570281/140‑1 (MQ=255)

                                                                                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCa                                              <  2:1507862/140‑1 (MQ=255)

                                                                                     tcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCa                                              <  1:1584231/140‑1 (MQ=255)

                                                                                        aacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATgg                                           <  2:838966/140‑1 (MQ=255)

                                                                                              tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAatga                                     <  1:122438/140‑1 (MQ=255)

                                                                                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAatgatg                                   <  2:1459973/140‑1 (MQ=255)

                                                                                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCCCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAatgatg                                   >  1:346459/1‑140 (MQ=255)

                                                                                                  ggCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCGTGGCAATGATGAg                                 <  1:845522/140‑1 (MQ=255)

                                                                                                  ggCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAg                                 <  2:270294/140‑1 (MQ=255)

                                                                                                  ggCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAg                                 <  2:665769/140‑1 (MQ=255)

                                                                                                   gCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGc                                <  1:839567/140‑1 (MQ=255)

                                                                                                   gCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGc                                <  2:132404/140‑1 (MQ=255)

                                                                                                   gCCCGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGAAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGc                                <  2:1308502/140‑1 (MQ=255)

                                                                                                    cccGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCg                               <  2:655672/140‑1 (MQ=255)

                                                                                                     ccGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGt                              <  1:851019/140‑1 (MQ=255)

                                                                                                     ccGTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGt                              >  2:18905/1‑140 (MQ=255)

                                                                                                       gTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTAc                            >  1:110382/1‑140 (MQ=255)

                                                                                                       gTTACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTAc                            >  1:1354960/1‑140 (MQ=255)

                                                                                                        ttACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAACCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACt                           >  1:75602/1‑140 (MQ=255)

                                                                                                        ttACTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGCTTCGCGTCATTTAGTCAACCTTGGTATGACACGGGAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGGAATGATGAGCGGAcc                           >  2:489833/1‑139 (MQ=255)

                                                                                                          aCTGAAACATGATGTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                         <  1:1585614/140‑1 (MQ=255)

                                                                                                                   tgatgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGgtgt                >  2:154428/1‑140 (MQ=255)

                                                                                                                    gatgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAACGTGGTGTc               >  1:884456/1‑140 (MQ=255)

                                                                                                                     atgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCg              >  2:1491118/1‑140 (MQ=255)

                                                                                                                     atgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCg              >  2:74838/1‑140 (MQ=255)

                                                                                                                      tgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc             >  2:1279495/1‑140 (MQ=255)

                                                                                                                      tgTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc             >  2:628632/1‑140 (MQ=255)

                                                                                                                       gTTTATAACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCa            <  1:18905/140‑1 (MQ=255)

                                                                                                                          tataACGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAAc         >  2:499661/1‑140 (MQ=255)

                                                                                                                             aaCGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCa      >  2:1375395/1‑140 (MQ=255)

                                                                                                                              aCGAACAGCATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAt     <  2:252668/140‑1 (MQ=255)

                                                                                                                                 aacagcATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTg  >  2:1183624/1‑140 (MQ=255)

TTAGTTATGAAGACTTCCATCGTCCTGAATTTACCAGCGTACCATTATTTAAAGATGAAATGGTGCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTG  >  NC_000913/84864‑85132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`