Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,588,397	G→A	P148L (CCT→CTT)	ydeS ←	putative fimbrial protein YdeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,588,397	0	G	A	100.0%	328.4 / NA	92	P148L (CCT→CTT)	ydeS	putative fimbrial protein YdeS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/55);  total (37/55)

CGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCAT  >  NC_000913/1588261‑1588529                                                                                                                                         |                                                                                                                                     cGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCa                                                                                                                                   <  1:1393182/140‑1 (MQ=255)      cTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAata                                                                                                                              <  1:239948/140‑1 (MQ=255)         aCAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtg                                                                                                                           <  1:45046/140‑1 (MQ=255)           aGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgtt                                                                                                                         <  2:1576898/140‑1 (MQ=255)           aGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgtt                                                                                                                         >  2:562356/1‑140 (MQ=255)            ggAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttc                                                                                                                        <  2:176936/140‑1 (MQ=255)             gAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttct                                                                                                                       <  1:190378/140‑1 (MQ=255)              aaCCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttctg                                                                                                                      >  2:1431311/1‑140 (MQ=255)               aCCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttctgt                                                                                                                     >  1:656796/1‑140 (MQ=255)               aCCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttctgt                                                                                                                     >  1:306360/1‑140 (MQ=255)                ccTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATtgttctgtt                                                                                                                    >  1:621958/1‑140 (MQ=255)                   ctTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGttctgttctg                                                                                                                 >  2:1175197/1‑140 (MQ=255)                    ttGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTgg                                                                                                                >  2:224426/1‑140 (MQ=255)                    ttGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTgg                                                                                                                <  2:1372879/140‑1 (MQ=255)                    ttGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTgg                                                                                                                <  2:418999/140‑1 (MQ=255)                     tgtgAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGt                                                                                                               <  2:1079054/140‑1 (MQ=255)                      gtgAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTa                                                                                                              <  2:754421/140‑1 (MQ=255)                       tgAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCTAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTAc                                                                                                             >  2:1160960/1‑140 (MQ=255)                          aGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAg                                                                                                          <  2:808046/140‑1 (MQ=255)                           gTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGt                                                                                                         >  2:742149/1‑140 (MQ=255)                            tCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTg                                                                                                        <  2:656796/140‑1 (MQ=255)                              ttttCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTggg                                                                                                      <  1:833552/140‑1 (MQ=255)                              ttttCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTggg                                                                                                      <  1:179045/140‑1 (MQ=255)                              ttttCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTggg                                                                                                      >  1:1520161/1‑140 (MQ=255)                                 tCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATc                                                                                                   <  2:1049801/140‑1 (MQ=255)                                   atatCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCa                                                                                                 <  2:437406/140‑1 (MQ=255)                                   atatCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCa                                                                                                 <  2:433652/140‑1 (MQ=255)                                   atatCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCa                                                                                                 <  2:774052/140‑1 (MQ=255)                                        tGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCa                                                                                            >  2:592234/1‑140 (MQ=255)                                          aCTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATc                                                                                          >  1:1276164/1‑140 (MQ=255)                                          aCTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATc                                                                                          >  2:380156/1‑140 (MQ=255)                                          aCTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATc                                                                                          >  2:783507/1‑140 (MQ=255)                                           ctctGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATcc                                                                                         <  2:608656/140‑1 (MQ=255)                                           ctctGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATcc                                                                                         <  2:322385/140‑1 (MQ=255)                                            tctGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATccc                                                                                        >  1:1105366/1‑140 (MQ=255)                                             ctGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCg                                                                                       <  1:148483/140‑1 (MQ=255)                                               gATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGc                                                                                     <  1:609425/140‑1 (MQ=255)                                               gATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGc                                                                                     <  1:861587/140‑1 (MQ=255)                                                attattGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCa                                                                                    >  1:634903/1‑140 (MQ=255)                                                attattGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCa                                                                                    >  2:1396152/1‑140 (MQ=255)                                                 ttattGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCAt                                                                                   <  1:1361952/140‑1 (MQ=255)                                                 ttattGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCAt                                                                                   <  2:1248467/140‑1 (MQ=255)                                                 ttattGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTAACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCAt                                                                                   <  1:138368/140‑1 (MQ=255)                                                    ttGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGaa                                                                                >  2:846872/1‑140 (MQ=255)                                                     tGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAg                                                                               >  2:1474010/1‑140 (MQ=255)                                                      gAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCGTCCCGGCATGAAGa                                                                              <  1:1102399/140‑1 (MQ=255)                                                        aaTTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATc                                                                            >  2:565572/1‑140 (MQ=255)                                                         aTTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCa                                                                           <  2:1520161/140‑1 (MQ=255)                                                          ttCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCAt                                                                          >  1:1084526/1‑140 (MQ=255)                                                               gggTAAAGGTTGCCGAAGCCCTCAGCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGt                                                                     >  2:1164115/1‑140 (MQ=255)                                                                    aaGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCAc                                                                <  2:306360/140‑1 (MQ=255)                                                                       gTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCgg                                                             <  1:224426/140‑1 (MQ=255)                                                                          gCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACg                                                          >  2:102263/1‑140 (MQ=255)                                                                          gCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACg                                                          >  1:198179/1‑140 (MQ=255)                                                                          gCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACg                                                          >  2:612113/1‑140 (MQ=255)                                                                           ccGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGc                                                         >  2:1028258/1‑140 (MQ=255)                                                                                gCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATAtt                                                    <  2:634903/140‑1 (MQ=255)                                                                                 cccTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATAttt                                                   >  2:1066177/1‑140 (MQ=255)                                                                                 cccTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATAttt                                                   <  1:742149/140‑1 (MQ=255)                                                                                    tCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCg                                                <  1:1160960/140‑1 (MQ=255)                                                                                    tCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCg                                                <  1:234142/140‑1 (MQ=255)                                                                                      aCCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTc                                              <  2:665633/140‑1 (MQ=255)                                                                                       ccAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTcc                                             <  1:1474010/140‑1 (MQ=255)                                                                                          gTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAat                                          >  2:560669/1‑140 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  2:664334/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  2:1105366/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  2:293600/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         >  2:1561805/1‑140 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  1:1175197/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  1:565572/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  1:562356/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         <  1:1431311/140‑1 (MQ=255)                                                                                           tCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAata                                         >  1:438937/1‑140 (MQ=255)                                                                                            cccGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAatat                                        >  2:886943/1‑140 (MQ=255)                                                                                                gATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCt                                    <  2:826031/140‑1 (MQ=255)                                                                                                     aCGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATAcc                               >  2:427942/1‑140 (MQ=255)                                                                                                      cGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATAccc                              >  1:352497/1‑140 (MQ=255)                                                                                                        gACTTCTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCaa                            <  2:889908/140‑1 (MQ=255)                                                                                                             cTGAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCgg                       <  2:785817/140‑1 (MQ=255)                                                                                                               gAGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAg                     >  1:1385731/1‑140 (MQ=255)                                                                                                                aGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAgg                    <  1:254947/140‑1 (MQ=255)                                                                                                                aGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAgg                    <  1:612113/140‑1 (MQ=255)                                                                                                                aGTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAgg                    <  1:886943/140‑1 (MQ=255)                                                                                                                 gTTGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGc                   <  2:785225/140‑1 (MQ=255)                                                                                                                   tGACTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCg                 <  1:1164115/140‑1 (MQ=255)                                                                                                                     aCTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTa               >  2:564037/1‑140 (MQ=255)                                                                                                                      cTTCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTAt              <  2:621958/140‑1 (MQ=255)                                                                                                                       ttCAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTAtt             >  1:489520/1‑140 (MQ=255)                                                                                                                         cAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGc           <  1:380156/140‑1 (MQ=255)                                                                                                                         cAGACGAGCGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGc           <  1:899568/140‑1 (MQ=255)                                                                                                                                 cGGAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCa   >  2:810062/1‑140 (MQ=255)                                                                                                                                  ggAGTAAAGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCAt  <  1:427942/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     CGTAGCTAACAGGAACCTCTTGTGAAGTCTTTTCATATCTGACTCTGATTATTGAAATTCAAGGGTAAAGGTTGCCGAAGCCCTCACCAGTCCCGGATTGACGGACTTCTGAGTTGACTTCAGACGAGCGGAGTAAGGCAAAATATTGTTCTGTTCTGGTACCAGTGGGATCCACTGCATCCCGGCATGAAGATCATTCAGTTTCACCGGACGCATATTTGCGTCCAATATTTCTATACCCAAACCGGAGGCCGTATTGCTTCCTTCAT  >  NC_000913/1588261‑1588529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑