Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,785,430	T→C	E561E (GAA→GAG)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,785,430	0	T	C	100.0%	284.4 / NA	75	E561E (GAA→GAG)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/41);  total (34/41)

CCGAGCATCGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCTTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATACGGTTGA  >  NC_000913/1785296‑1785566                                                                                                                                       |                                                                                                                                         ccGAGCATCGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCtcagt                                                                                                                                     <  2:1470896/140‑1 (MQ=255)    aGCATCGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCtcagtcag                                                                                                                                  <  2:1521136/140‑1 (MQ=255)       aTCGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAgacg                                                                                                                               <  1:1518484/140‑1 (MQ=255)         cGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACGCAGCGTCGCGATCCCCTCAGTCAgacgac                                                                                                                             <  2:351143/140‑1 (MQ=255)         cGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAgacgac                                                                                                                             <  1:553649/140‑1 (MQ=255)          gggTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGAcc                                                                                                                            <  1:182826/140‑1 (MQ=255)              tctcTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACg                                                                                                                        <  1:766285/140‑1 (MQ=255)               ctctTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGt                                                                                                                       >  1:452950/1‑140 (MQ=255)                     aTCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAAtt                                                                                                                 >  1:1502307/1‑140 (MQ=255)                     aTCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAAtt                                                                                                                 >  1:24853/1‑140 (MQ=255)                       cTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCa                                                                                                               <  2:1409495/140‑1 (MQ=255)                       cTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCa                                                                                                               >  2:1274592/1‑140 (MQ=255)                         ggCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCAcg                                                                                                             <  2:1151513/140‑1 (MQ=255)                          gCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCAcgc                                                                                                            >  2:310165/1‑140 (MQ=255)                          gCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCAcgc                                                                                                            <  2:1095467/140‑1 (MQ=255)                                    cTCACCACCGACCAGAATGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGACGCGATCCCCTCAGTCAGACGCCCAACGTAAAATTCACGCGGAGAATCaa                                                                                                  >  2:336771/1‑140 (MQ=255)                                      caccacCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCaaaa                                                                                                <  2:535474/140‑1 (MQ=255)                                      caccacCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCaaaa                                                                                                <  2:867593/140‑1 (MQ=255)                                       accaccGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAAc                                                                                               >  1:1270474/1‑140 (MQ=255)                                       accaccGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAAc                                                                                               <  2:601286/140‑1 (MQ=255)                                        ccaccGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAAcc                                                                                              <  2:399553/140‑1 (MQ=255)                                        ccaccGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAAcc                                                                                              <  2:1405854/140‑1 (MQ=255)                                          accGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCtt                                                                                            >  1:783554/1‑140 (MQ=255)                                          accGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCtt                                                                                            >  2:155552/1‑140 (MQ=255)                                              aCCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTtcat                                                                                        >  2:706984/1‑140 (MQ=255)                                               ccAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCAGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTtcatc                                                                                       >  2:912998/1‑140 (MQ=255)                                                      ggCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCCCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTcgc                                                                                >  2:122735/1‑140 (MQ=255)                                                        cATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCgg                                                                              >  1:1326262/1‑140 (MQ=255)                                                           atTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGAtt                                                                           <  1:1160563/140‑1 (MQ=255)                                                            ttcgttcgATTTAAAATCAGAGAGACGGACAATAACGCGCTTCGGATAAAACGCGGCACCTAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGTAGAATCAAAACCTTTCATCATCTCGCGGAttt                                                                          >  2:1508850/1‑140 (MQ=255)                                                             tcgttcgATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTc                                                                         >  2:680304/1‑140 (MQ=255)                                                                 tcgATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGttt                                                                     >  1:862529/1‑140 (MQ=255)                                                                 tcgATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGttt                                                                     >  1:165913/1‑140 (MQ=255)                                                                  cgATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGtttt                                                                    >  1:956864/1‑140 (MQ=255)                                                                    aTTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGc                                                                  >  1:452344/1‑140 (MQ=255)                                                                        aaaaTCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACt                                                              <  2:1061576/140‑1 (MQ=255)                                                                         aaaTCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTg                                                             <  2:452344/140‑1 (MQ=255)                                                                         aaaTCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTg                                                             <  2:1348695/140‑1 (MQ=255)                                                                            tCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCgg                                                          <  2:163815/140‑1 (MQ=255)                                                                               gagagaCGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTc                                                       >  2:7146/1‑140 (MQ=255)                                                                               gagagaCGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTc                                                       >  1:1316780/1‑140 (MQ=255)                                                                               gagagaCGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTc                                                       >  1:1124990/1‑140 (MQ=255)                                                                               gagagaCGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTc                                                       >  2:616744/1‑140 (MQ=255)                                                                                    aCGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGAt                                                  >  1:789697/1‑140 (MQ=255)                                                                                     cGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATc                                                 >  2:569533/1‑140 (MQ=255)                                                                                       gACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGt                                               <  2:1437649/140‑1 (MQ=255)                                                                                       gACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGt                                               <  2:24853/140‑1 (MQ=255)                                                                                         cAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCa                                             <  1:122735/140‑1 (MQ=255)                                                                                         cAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCa                                             <  1:155552/140‑1 (MQ=255)                                                                                         cAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCa                                             <  1:680304/140‑1 (MQ=255)                                                                                              aCGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCaaa                                           <  2:696857/137‑1 (MQ=255)                                                                                              aCGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCaaa                                           >  1:696857/1‑137 (MQ=255)                                                                                                 cgcTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAg                                     <  1:1238917/140‑1 (MQ=255)                                                                                                 cgcTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAg                                     <  1:950862/140‑1 (MQ=255)                                                                                                  gcTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGc                                    >  1:408144/1‑140 (MQ=255)                                                                                                  gcTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGc                                    <  2:89451/140‑1 (MQ=255)                                                                                                  gcTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTC‑GACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCa                                   >  1:585073/1‑140 (MQ=255)                                                                                                   cTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCa                                   >  1:1402137/1‑140 (MQ=255)                                                                                                   cTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCa                                   >  1:440736/1‑140 (MQ=255)                                                                                                    ttCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAg                                  >  2:775305/1‑140 (MQ=255)                                                                                                     tCGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGt                                 <  1:1287779/140‑1 (MQ=255)                                                                                                      cGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTg                                >  2:1331888/1‑140 (MQ=255)                                                                                                      cGGATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTg                                >  1:29857/1‑140 (MQ=255)                                                                                                        gATAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTgcg                              <  2:195235/140‑1 (MQ=255)                                                                                                         aTAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTgcgc                             <  1:706984/140‑1 (MQ=255)                                                                                                          tAAAACGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAGCCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGcgcg                            <  1:383544/140‑1 (MQ=255)                                                                                                           aaaaCGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCgt                           <  2:789697/140‑1 (MQ=255)                                                                                                              aCGCGGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCgtggg                        >  1:979001/1‑140 (MQ=255)                                                                                                                gcgGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCgtgggtg                      <  2:165913/140‑1 (MQ=255)                                                                                                                gcgGCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCgtgggtg                      <  1:155944/140‑1 (MQ=255)                                                                                                                  ggCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGa                    >  2:320795/1‑140 (MQ=255)                                                                                                                   gCACCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGAc                   >  1:1298196/1‑140 (MQ=255)                                                                                                                     aCCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGc                 <  1:1089019/140‑1 (MQ=255)                                                                                                                     aCCCAGCGTCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGc                 <  2:1502307/140‑1 (MQ=255)                                                                                                                             tCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATa         <  2:783554/140‑1 (MQ=255)                                                                                                                             tCGCGATCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATa         <  1:569533/140‑1 (MQ=255)                                                                                                                                   tCCCCTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATACGGTtg   <  1:1356192/140‑1 (MQ=255)                                                                                                                                    ccccTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATACGGTtga  <  2:29857/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         CCGAGCATCGGGTTCTCTTCATCTGGCTCGTAACGCTCACCACCGACCAGGTTGGCATATTCGTTCGATTTAAAATCAGAGAGACGGACAATGACGCGCTTCGGATAAAACGCGGCACCCAGCGTCGCGATCCCTTCAGTCAGACGACCAACGTAAAATTCACGCGGAGAATCAAAACCTTTCATCATCTCGCGGATTTCGTTTTGCAACTGCGGTTCCTGATCGTCAAACTCAAGCAGTGCGCGTGGGTGGACGCCAATCATACGGTTGA  >  NC_000913/1785296‑1785566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑