Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,827,696	T→C	E188E (GAA→GAG)	astB ←	N‑succinylarginine dihydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,827,696	0	T	C	100.0%	203.6 / NA	57	E188E (GAA→GAG)	astB	N‑succinylarginine dihydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/26);  total (31/26)

AAAATCACCTGTTGGGGATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACG  >  NC_000913/1827559‑1827831                                                                                                                                          |                                                                                                                                        aaaaTCACCTGTTGGGGATTCCCCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTc                                                                                                                                       <  1:1109196/140‑1 (MQ=255)  aaaTCACCTGTTGGGGATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCa                                                                                                                                      <  2:908159/140‑1 (MQ=255)  aaaTCACCTGTTGGGGATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCa                                                                                                                                      >  2:775539/1‑140 (MQ=255)            ttGGGGATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGAc                                                                                                                            >  2:942796/1‑140 (MQ=255)               gggATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGAccgc                                                                                                                         <  2:1021006/140‑1 (MQ=255)                  aTTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCga                                                                                                                      <  1:976697/140‑1 (MQ=255)                      aCCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACg                                                                                                                  >  1:985660/1‑140 (MQ=255)                          gATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATtg                                                                                                              >  1:1477902/1‑140 (MQ=255)                              cAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGt                                                                                                          <  2:885327/140‑1 (MQ=255)                                 ccTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTg                                                                                                       >  1:859777/1‑140 (MQ=255)                                 ccTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTg                                                                                                       >  2:412488/1‑140 (MQ=255)                                   ttGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTgcc                                                                                                     <  1:338158/140‑1 (MQ=255)                                     gCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTgccgc                                                                                                   >  2:1407503/1‑140 (MQ=255)                                     gCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTgccgc                                                                                                   >  1:1244861/1‑140 (MQ=255)                                     gCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTgccgc                                                                                                   >  1:493756/1‑140 (MQ=255)                                           gCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCtc                                                                                             >  1:355792/1‑140 (MQ=255)                                                  tGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCg                                                                                      <  1:842051/140‑1 (MQ=255)                                                   ggCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGa                                                                                     <  1:1508606/140‑1 (MQ=255)                                                   ggCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGa                                                                                     <  1:775539/140‑1 (MQ=255)                                                    gCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATCCCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAg                                                                                    <  2:1328615/140‑1 (MQ=255)                                                    gCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAg                                                                                    <  1:1140424/140‑1 (MQ=255)                                                    gCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAg                                                                                    <  1:942796/140‑1 (MQ=255)                                                     cTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGc                                                                                   <  1:52760/140‑1 (MQ=255)                                                     cTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGc                                                                                   <  2:701895/140‑1 (MQ=255)                                                     cTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGc                                                                                   <  1:705410/140‑1 (MQ=255)                                                       tCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTTATCACCGAGCaa                                                                                 >  1:1386866/1‑140 (MQ=255)                                                        cgcgAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAAc                                                                                >  2:1057538/1‑140 (MQ=255)                                                         gcgAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACg                                                                               <  2:1565815/140‑1 (MQ=255)                                                         gcgAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACg                                                                               <  2:1477902/140‑1 (MQ=255)                                                         gcgAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACg                                                                               <  1:1050815/140‑1 (MQ=255)                                                          cgAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGc                                                                              >  1:72627/1‑140 (MQ=255)                                                                            tAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCTTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGa                                                            >  1:717068/1‑140 (MQ=255)                                                                              aCGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAAt                                                          <  2:607032/140‑1 (MQ=255)                                                                                    aGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGc                                                    >  1:782648/1‑140 (MQ=255)                                                                                    aGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGc                                                    >  2:502654/1‑140 (MQ=255)                                                                                                  tGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTtcg                                      <  1:490470/140‑1 (MQ=255)                                                                                                    ccTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTtcgtc                                    >  1:793714/1‑140 (MQ=255)                                                                                                    ccTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTtcgtc                                    >  1:721406/1‑140 (MQ=255)                                                                                                    ccTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTtcgtc                                    >  2:1532416/1‑140 (MQ=255)                                                                                                     cttcttCTCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTtcgtcg                                   >  1:528236/1‑140 (MQ=255)                                                                                                        cttctCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTa                                >  2:344562/1‑140 (MQ=255)                                                                                                         ttctCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTaa                               >  2:1311721/1‑140 (MQ=255)                                                                                                         ttctCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTaa                               >  2:1243667/1‑140 (MQ=255)                                                                                                         ttctCGCCCGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTaa                               >  2:567804/1‑140 (MQ=255)                                                                                                               cccGTAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGGTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCg                         >  1:1502996/1‑140 (MQ=255)                                                                                                                   tAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCttt                     >  1:191827/1‑140 (MQ=255)                                                                                                                   tAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCttt                     >  2:1461656/1‑140 (MQ=255)                                                                                                                   tAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCttt                     >  1:479861/1‑140 (MQ=255)                                                                                                                   tAGACAAAAAGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCttt                     >  1:1564276/1‑140 (MQ=255)                                                                                                                         aaaaGTTGCATCCCCGCCTCGCCGCAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCCCCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAg               <  2:1180999/140‑1 (MQ=255)                                                                                                                         aaaaGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTTATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAg               <  2:1386866/140‑1 (MQ=255)                                                                                                                         aaaaGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAg               <  2:355792/140‑1 (MQ=255)                                                                                                                         aaaaGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAg               <  2:1093779/140‑1 (MQ=255)                                                                                                                         aaaaGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAg               <  1:1532416/140‑1 (MQ=255)                                                                                                                          aaaGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGc              >  1:1444283/1‑140 (MQ=255)                                                                                                                            aGTTGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGa            <  1:745655/140‑1 (MQ=255)                                                                                                                              ttGCATACCCGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGAtt          <  1:1057538/140‑1 (MQ=255)                                                                                                                                      ccGGCTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACg  >  2:307199/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AAAATCACCTGTTGGGGATTCACCTGATTCAGCCTTGCCACCGCCTCGCTGGCTTCGCGAGTCTGTCGCGCCGGATAACGGGAAGGCCGGGTATCATTGCCTTCTTCTCGCCCGTAGACAAAAAGTTGCATACCCGGTTCACCGTAATGACCGCCGAGACGATTGTGGTTTGCCGCCCCCTCATCACCGAGCAACGCTACCTGTGGCAACGCCGAATGGACGCTAAATTTCTCTTCGTCGTTAAAAATCGCTTTTAACAGCGATTCAGTGACG  >  NC_000913/1827559‑1827831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑