Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	92,651	C→T	A413A (GCC→GCT)	ftsI →	peptidoglycan DD‑transpeptidase FtsI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	92,651	0	C	T	100.0%	314.9 / NA	91	A413A (GCC→GCT)	ftsI	peptidoglycan DD‑transpeptidase FtsI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (50/41);  total (50/41)

GTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCT  >  NC_000913/92516‑92787                                                                                                                                        |                                                                                                                                         gTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCt                                                                                                                                      <  2:462870/140‑1 (MQ=255)              tCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTa                                                                                                                         <  1:1529856/140‑1 (MQ=255)              tCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTa                                                                                                                         <  1:1543074/140‑1 (MQ=255)              tCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTa                                                                                                                         <  1:1099406/140‑1 (MQ=255)                cTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACg                                                                                                                       <  1:289897/140‑1 (MQ=255)                cTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACg                                                                                                                       <  2:495722/140‑1 (MQ=255)                    gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCt                                                                                                                   <  2:1314679/140‑1 (MQ=255)                    gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCt                                                                                                                   <  1:87585/140‑1 (MQ=255)                    gCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCt                                                                                                                   <  2:1328960/140‑1 (MQ=255)                     cGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTa                                                                                                                  <  2:566793/140‑1 (MQ=255)                          gtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGt                                                                                                             >  2:1102364/1‑140 (MQ=255)                          gtagATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGt                                                                                                             <  1:832368/140‑1 (MQ=255)                            agATACTTACTCACGTTTTGGACTGGGAGAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTaa                                                                                                           <  1:1226772/140‑1 (MQ=255)                             gATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAac                                                                                                          >  1:1334324/1‑140 (MQ=255)                              aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                                                                                         <  1:1327189/140‑1 (MQ=255)                              aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                                                                                         <  2:554402/140‑1 (MQ=255)                              aTACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAaca                                                                                                         <  1:1203254/140‑1 (MQ=255)                               tacttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAacac                                                                                                        >  1:237260/1‑140 (MQ=255)                                  ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAt                                                                                                     >  2:321093/1‑140 (MQ=255)                                  ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAt                                                                                                     >  1:936957/1‑140 (MQ=255)                                  ttactCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAt                                                                                                     <  2:209680/140‑1 (MQ=255)                                   tactCACGTTTTGGACTGGGAAAAGCGACTAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCAtt                                                                                                    >  1:24649/1‑140 (MQ=255)                                    actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                                                                                   >  1:1561913/1‑140 (MQ=255)                                    actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                                                                                   >  1:1347669/1‑140 (MQ=255)                                    actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                                                                                   >  2:167598/1‑140 (MQ=255)                                    actCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTa                                                                                                   >  1:1317027/1‑140 (MQ=255)                                     ctCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTAc                                                                                                  >  2:1565180/1‑140 (MQ=255)                                          gTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTa                                                                                             >  2:1391324/1‑140 (MQ=255)                                            tttGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAgc                                                                                           >  1:656384/1‑140 (MQ=255)                                             ttGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAgcg                                                                                          <  2:748212/140‑1 (MQ=255)                                              tGGACTGGGAAAAGCGACCAATTTGGGGATGGTCGGCGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGCCTAATGGTAACACCATTACAGTCAgcgc                                                                                         >  2:814004/1‑140 (MQ=255)                                                    gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTcc                                                                                   >  2:212688/1‑139 (MQ=255)                                                    gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCt                                                                                   >  1:880030/1‑140 (MQ=255)                                                    gggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCt                                                                                   >  1:1168379/1‑140 (MQ=255)                                                     ggAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTa                                                                                  <  1:563152/140‑1 (MQ=255)                                                       aaaaGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACg                                                                                <  2:519437/140‑1 (MQ=255)                                                       aaaaGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACg                                                                                <  2:2719/140‑1 (MQ=255)                                                          aGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCaa                                                                             >  1:1474590/1‑140 (MQ=255)                                                          aGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCaa                                                                             <  2:218633/140‑1 (MQ=255)                                                          aGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCaa                                                                             <  1:493333/140‑1 (MQ=255)                                                               cccaTTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGACTAATGGCAACACCATTACAGGTAGCGCGAGTCTACGCAACTATc                                                                        >  2:1176436/4‑140 (MQ=255)                                                               ccAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATc                                                                        >  2:229638/1‑140 (MQ=255)                                                                cAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCg                                                                       <  1:1252008/140‑1 (MQ=255)                                                                      ggggTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCt                                                                 <  2:1121265/140‑1 (MQ=255)                                                                         gTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACg                                                              >  1:1239725/1‑140 (MQ=255)                                                                         gTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACg                                                              >  1:1373726/1‑140 (MQ=255)                                                                           tGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGc                                                            <  1:771584/140‑1 (MQ=255)                                                                             gTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAt                                                          >  1:1104321/1‑140 (MQ=255)                                                                             gTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAt                                                          >  1:322618/1‑140 (MQ=255)                                                                             gTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAt                                                          >  1:107978/1‑140 (MQ=255)                                                                             gTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCAt                                                          >  2:1107881/1‑140 (MQ=255)                                                                                ggaggaCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTGCCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTa                                                       <  2:235326/140‑1 (MQ=255)                                                                                ggAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTa                                                       <  2:24649/140‑1 (MQ=255)                                                                                  agaACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATc                                                     >  1:1546672/1‑140 (MQ=255)                                                                                     aCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGcc                                                  <  2:237260/140‑1 (MQ=255)                                                                                     aCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGcc                                                  <  2:1317027/140‑1 (MQ=255)                                                                                     aCGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGcc                                                  <  1:128444/140‑1 (MQ=255)                                                                                       gCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACACAACTATCGGCAGCTACGGCATTTATCGCCCa                                                <  1:1339309/140‑1 (MQ=255)                                                                                          gTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTg                                             <  2:1546672/140‑1 (MQ=255)                                                                                           tGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGt                                            >  1:999704/1‑140 (MQ=255)                                                                                             gCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCg                                          <  2:101095/140‑1 (MQ=255)                                                                                              cTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGa                                         >  2:1130332/1‑140 (MQ=255)                                                                                              cTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGa                                         >  1:361832/1‑140 (MQ=255)                                                                                               ttATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGAt                                        <  1:383668/140‑1 (MQ=255)                                                                                                 atatCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTa                                      >  2:1127633/1‑140 (MQ=255)                                                                                                     ccTCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCaa                                  >  1:1012468/1‑140 (MQ=255)                                                                                                       tCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAg                                >  2:241563/1‑140 (MQ=255)                                                                                                       tCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAg                                >  1:282593/1‑140 (MQ=255)                                                                                                       tCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAg                                >  1:25793/1‑140 (MQ=255)                                                                                                       tCAAAAACAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAg                                >  1:227363/1‑140 (MQ=255)                                                                                                       tCAAAAACAACGGTGGGCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAg                                >  2:9805/1‑140 (MQ=255)                                                                                                         aaaaaCAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGtt                              >  1:698300/1‑140 (MQ=255)                                                                                                           aaaCAACGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGa                            >  2:1252862/1‑140 (MQ=255)                                                                                                               aacGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccc                        <  2:1347669/140‑1 (MQ=255)                                                                                                                acGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAccccc                       >  1:441546/1‑140 (MQ=255)                                                                                                                 cGGTGGTCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGAcccccc                      <  1:1107881/140‑1 (MQ=255)                                                                                                                   gtggtCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCgg                    >  2:1077977/1‑140 (MQ=255)                                                                                                                    tggtCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGt                   <  1:1102364/140‑1 (MQ=255)                                                                                                                     ggtCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGtt                  <  2:1104321/140‑1 (MQ=255)                                                                                                                     ggtCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGtt                  <  2:525580/140‑1 (MQ=255)                                                                                                                     ggtCTGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGtt                  <  1:212688/140‑1 (MQ=255)                                                                                                                         tGACATAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCg              >  1:1092919/1‑140 (MQ=255)                                                                                                                             aTAGAGAGGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGa          <  2:1334324/140‑1 (MQ=255)                                                                                                                               agagagGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAAc        <  2:107978/140‑1 (MQ=255)                                                                                                                                gagagGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACg       >  2:129867/1‑140 (MQ=255)                                                                                                                                 agagGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgt      >  1:1517619/1‑140 (MQ=255)                                                                                                                                 agagGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgt      >  1:1337664/1‑140 (MQ=255)                                                                                                                                   agGGCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACgtgt    >  1:843172/1‑140 (MQ=255)                                                                                                                                     ggCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCt  >  1:277654/1‑140 (MQ=255)                                                                                                                                     ggCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCt  >  1:1532861/1‑140 (MQ=255)                                                                                                                                     ggCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCt  >  1:712504/1‑140 (MQ=255)                                                                                                                                     ggCTACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCt  >  1:10772/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         GTTAGCGATGCCGTCCTCAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTATATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCATTACAGTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTCCCGGTGAACGTGTCT  >  NC_000913/92516‑92787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑