Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,996,183	T→C	N217S (AAC→AGC)	sdiA ←	DNA‑binding transcriptional dual regulator SdiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,996,183	0	T	C	100.0%	239.2 / NA	63	N217S (AAC→AGC)	sdiA	DNA‑binding transcriptional dual regulator SdiA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/27);  total (36/27)

CGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996051‑1996318                                                                                                                                     |                                                                                                                                        cGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtg                                                                                                                                  >  2:339559/1‑140 (MQ=255)  ggCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtgg                                                                                                                                 >  2:646524/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:877396/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:752349/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  2:838306/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:293270/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  1:269479/1‑140 (MQ=255)   gCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCtggt                                                                                                                                >  2:603535/1‑140 (MQ=255)         aGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAAt                                                                                                                          <  1:1290484/140‑1 (MQ=255)         aGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAAt                                                                                                                          <  2:501749/140‑1 (MQ=255)           cGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTg                                                                                                                        <  2:539802/140‑1 (MQ=255)           cGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTg                                                                                                                        <  1:442671/140‑1 (MQ=255)             tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  1:1415544/1‑140 (MQ=255)             tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      <  1:568297/140‑1 (MQ=255)             tGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGAc                                                                                                                      >  2:872895/1‑140 (MQ=255)               ccTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCg                                                                                                                    <  1:334703/140‑1 (MQ=255)               ccTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCg                                                                                                                    <  2:315084/140‑1 (MQ=255)                    cAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATtc                                                                                                               >  1:434656/1‑140 (MQ=255)                       ccGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCa                                                                                                            <  2:788151/140‑1 (MQ=255)                          gTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCagag                                                                                                         >  2:222881/1‑140 (MQ=255)                                 cATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACa                                                                                                  >  1:964938/1‑140 (MQ=255)                                      ggCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatc                                                                                             >  2:1243434/1‑140 (MQ=255)                                        cACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAatcat                                                                                           >  2:697369/1‑140 (MQ=255)                                             aGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTa                                                                                      <  1:907525/140‑1 (MQ=255)                                                 cAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTAtctc                                                                                  >  2:210659/1‑140 (MQ=255)                                                    aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                               >  2:237862/1‑140 (MQ=255)                                                    aaaaGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTctgc                                                                               <  2:1415544/140‑1 (MQ=255)                                                        gagaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGAt                                                                           <  1:986116/140‑1 (MQ=255)                                                         agaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATg                                                                          >  2:633593/1‑140 (MQ=255)                                                          gaTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGt                                                                         >  2:282168/1‑140 (MQ=255)                                                           aTCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGtt                                                                        <  1:1076387/140‑1 (MQ=255)                                                              aaaTTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTc                                                                     >  1:1131994/1‑140 (MQ=255)                                                              aaaTTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTc                                                                     >  2:155681/1‑140 (MQ=255)                                                                aTTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTccc                                                                   <  1:618830/140‑1 (MQ=255)                                                                   aaGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTc                                                                <  2:1066442/140‑1 (MQ=255)                                                                       cAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTccgcc                                                            >  1:1113181/1‑140 (MQ=255)                                                                          tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         <  2:1067644/140‑1 (MQ=255)                                                                          tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         <  1:140436/140‑1 (MQ=255)                                                                          tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         >  1:1497545/1‑140 (MQ=255)                                                                          tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTc                                                         >  1:1529936/1‑140 (MQ=255)                                                                          tAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGAGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTACCGCCGTc                                                         >  1:627391/1‑140 (MQ=255)                                                                             cGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCAc                                                      <  1:872895/140‑1 (MQ=255)                                                                               gCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCt                                                    >  2:267285/1‑140 (MQ=255)                                                                                ccGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTc                                                   >  1:763299/1‑140 (MQ=255)                                                                                                   ggTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCt                                >  2:1012118/1‑140 (MQ=255)                                                                                                   ggTCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCt                                >  1:167126/1‑140 (MQ=255)                                                                                                     tCTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTC‑TTTTCCCGCTTGc                             >  1:1405661/1‑140 (MQ=255)                                                                                                      cTTATTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGc                             >  2:1199148/1‑140 (MQ=255)                                                                                                         aTTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGa                          >  2:260386/1‑140 (MQ=255)                                                                                                         aTTTGGTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGa                          >  2:628034/1‑140 (MQ=255)                                                                                                             ggTGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAAtt                      >  2:520085/1‑140 (MQ=255)                                                                                                               tGCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCa                    >  1:156188/1‑140 (MQ=255)                                                                                                                gCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAt                   <  1:222881/140‑1 (MQ=255)                                                                                                                gCATTAATTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCAt                   <  2:1113181/140‑1 (MQ=255)                                                                                                                     aaTTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTg              <  2:752349/140‑1 (MQ=255)                                                                                                                      aTTTTTTTCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTgg             <  2:877396/140‑1 (MQ=255)                                                                                                                       tttttttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGc            <  2:895041/140‑1 (MQ=255)                                                                                                                            ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCTCCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  2:627391/140‑1 (MQ=255)                                                                                                                            ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  1:210659/140‑1 (MQ=255)                                                                                                                            ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  1:603535/140‑1 (MQ=255)                                                                                                                            ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  2:434656/140‑1 (MQ=255)                                                                                                                            ttCTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcat       <  2:293270/140‑1 (MQ=255)                                                                                                                              cTGCATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGtcatca     <  1:1012118/140‑1 (MQ=255)                                                                                                                                 cATGCTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTa  >  2:1406670/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CGGCTGATAGCGTGCCTTTCAGCCGGTTTTTGCATCTGGCACGCAGGACAGAAAAGAGATCAAATTAAGCCAGTAGCGGCCGCGTAACAGGCAACCTGGGTCTTATTTGGTGCATTAATTTTTTTCTGCATGTTTTTCTGGTGGAAATTGACCGTATTCTCAGAGATTGACAAAATCATCGCTATCTCTGCTGATGTTTTCCCTTCCGCCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTA  >  NC_000913/1996051‑1996318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑