Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,168,392	A→G	intergenic (‑86/‑320)	yegR ← / → yegS	uncharacterized protein YegR/lipid kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,168,392	0	A	G	100.0%	133.5 / NA	36	intergenic (‑86/‑320)	yegR/yegS	uncharacterized protein YegR/lipid kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/12);  total (24/12)

CATAATAAAAATACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATG  >  NC_000913/2168262‑2168522                                                                                                                                   |                                                                                                                                   cATAATAAAAATACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGtt                                                                                                                           >  2:1584423/1‑140 (MQ=255) cATAATAAAAATACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGtt                                                                                                                           <  1:30460/140‑1 (MQ=255)                aattaatGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTcc                                                                                                            >  1:1269748/1‑140 (MQ=255)                      ttatatAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATa                                                                                                      >  1:723221/3‑140 (MQ=255)                      tGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATa                                                                                                      >  2:902801/1‑140 (MQ=255)                      tGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATa                                                                                                      >  1:441554/1‑140 (MQ=255)                           taGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAg                                                                                                 >  2:1066100/1‑140 (MQ=255)                              cagcTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGaa                                                                                              >  1:1050014/1‑140 (MQ=255)                              cagcTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGaa                                                                                              >  1:1435872/1‑140 (MQ=255)                                  tATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTAc                                                                                          >  2:1524027/1‑140 (MQ=255)                                   aTCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACg                                                                                         >  2:1562043/1‑140 (MQ=255)                                                        cccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGt                                                                    <  1:1584423/140‑1 (MQ=255)                                                        cccATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGt                                                                    <  1:902801/140‑1 (MQ=255)                                                           aTTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAAc                                                                 >  2:1039903/1‑140 (MQ=255)                                                                     aaCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATggggg                                                       <  1:1524027/140‑1 (MQ=255)                                                                      aCGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGc                                                      >  1:780516/1‑140 (MQ=255)                                                                          cacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTa                                                  <  2:868276/140‑1 (MQ=255)                                                                          cacTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTa                                                  <  2:441554/140‑1 (MQ=255)                                                                           acTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTaa                                                 >  1:1299421/1‑140 (MQ=255)                                                                               tctAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAatata                                             <  1:1040709/140‑1 (MQ=255)                                                                                    tgtTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAAtagattata                                        >  2:1147096/1‑140 (MQ=255)                                                                                      tttAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATatattatatt                                      >  1:1273137/1‑140 (MQ=255)                                                                                         aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                                   >  2:1468715/1‑140 (MQ=255)                                                                                         aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                                   <  2:723221/140‑1 (MQ=255)                                                                                         aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGaa                                   >  1:248581/1‑140 (MQ=255)                                                                                         aaaTATAATAATGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTNNNNNGTAACTCCATGGGGGCTTTAATATATTATATTGaa                                   >  2:1191482/1‑140 (MQ=255)                                                                                                    tGGCATGATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGt                        >  1:1458197/1‑140 (MQ=255)                                                                                                          gATTATTATAATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGtt                  <  2:1050014/140‑1 (MQ=255)                                                                                                                tataATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAatat            <  2:1299421/140‑1 (MQ=255)                                                                                                                  taATTGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTa          <  2:1435872/140‑1 (MQ=255)                                                                                                                     ttGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt       >  2:28684/1‑140 (MQ=255)                                                                                                                     ttGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt       <  2:366329/140‑1 (MQ=255)                                                                                                                     ttGAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGt       >  1:1584132/1‑140 (MQ=255)                                                                                                                       gAATAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGtat     >  1:156263/1‑140 (MQ=255)                                                                                                                          tAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATg  >  1:663687/1‑140 (MQ=255)                                                                                                                          tAGGATTATGATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATg  >  1:587199/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   CATAATAAAAATACTAATTAATGATATAGCAGCTATCTTTTTCATCTCACCTTCCCCCATTAAATACCAACGACACTCTCTAGTGTTTAAATATAATAATGGCATGATTATTATAATTGAATAGGATTATAATAAATGTTCTGTACAACATTTCCTACATAAGTAGGAATTACGGACATTGAGGCCCTTCAGGGTAACTCCATGGGGGCTTTAATATATTATATTGAAGATGCCACTGTTTAGTTGAATATTAGGTATATG  >  NC_000913/2168262‑2168522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑