Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	175,792	A→G	Y229C (TAC→TGC)	clcA →	chloride:H(+) antiporter ClcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	175,792	0	A	G	100.0%	202.8 / NA	54	Y229C (TAC→TGC)	clcA	chloride:H(+) antiporter ClcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/26);  total (28/26)

CGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTACCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGC  >  NC_000913/175657‑175926                                                                                                                                        |                                                                                                                                       cGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGGATTTTGTTTATTATCGAAGAGATGCGTCCGCAGGTTCGCTATACGTTAATTTCGATTAAAGCGGGATTTATTGGTGTCATTATGTCCACCCTTATGTGCCgg                                                                                                                                    >  1:1116518/1‑140 (MQ=255)   gggCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTTTCGAAGAGATTCGTCCCCAGTTTTGCTATAAGGTAAATTCGATTAAAGGGGGATTTTTTTGTGTCATTATGTCGACCATTATGTGCCCGag                                                                                                                                  >  2:645422/1‑139 (MQ=255)       tggctgCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGAttttt                                                                                                                              <  1:502104/140‑1 (MQ=255)            gCGGCCTTTAACGCGCCGATGGCGGGTATTTTGTTTAATATCAAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCa                                                                                                                         <  1:1445191/140‑1 (MQ=255)               gCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGGATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGa                                                                                                                      >  1:75736/1‑140 (MQ=255)                   ttAACGCGCCGCTGGCGGGGATTTTGTTTATTATCGAAGAGATGCGTCCGCCGGTTCGCTATACGTTAATTTCGATTAAAGCGGGATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAggt                                                                                                                  >  2:177602/1‑138 (MQ=255)                     aaCGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGc                                                                                                                <  2:97587/140‑1 (MQ=255)                      aCGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCg                                                                                                               <  1:1540490/140‑1 (MQ=255)                            cgcTGGCGGGGATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGttgatt                                                                                                         >  2:1062902/1‑140 (MQ=255)                                   gggTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCg                                                                                                  <  1:1238591/140‑1 (MQ=255)                                                  tatCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATg                                                                                   <  2:786533/140‑1 (MQ=255)                                                    tCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATgcg                                                                                 <  2:1133104/140‑1 (MQ=255)                                                    tCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATgcg                                                                                 <  2:806649/140‑1 (MQ=255)                                                    tCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATgcg                                                                                 <  2:1093131/140‑1 (MQ=255)                                                    tCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATgcg                                                                                 <  1:744419/140‑1 (MQ=255)                                                     cGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATgcgc                                                                                >  1:1048242/1‑140 (MQ=255)                                                      gAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGcgcc                                                                               <  2:1201263/140‑1 (MQ=255)                                                       aaGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGcgccg                                                                              >  1:623345/1‑140 (MQ=255)                                                            aTGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTaa                                                                         >  2:1234592/1‑140 (MQ=255)                                                            aTGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTaa                                                                         >  1:643252/1‑140 (MQ=255)                                                             tGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAAt                                                                        <  1:995014/140‑1 (MQ=255)                                                              gCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATa                                                                       >  2:1562524/1‑140 (MQ=255)                                                              gCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATa                                                                       >  2:207339/1‑140 (MQ=255)                                                              gCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATa                                                                       <  1:1062902/140‑1 (MQ=255)                                                                  ccGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGATAAACTTTCTGATGCGCCGCTTAATACGCt                                                                   >  2:565871/1‑140 (MQ=255)                                                                   cGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCtg                                                                  <  2:815841/140‑1 (MQ=255)                                                                          tCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCttt                                                           <  1:645422/140‑1 (MQ=255)                                                                                    ttAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCt                                                 <  2:672929/140‑1 (MQ=255)                                                                                    ttAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCt                                                 <  2:849888/140‑1 (MQ=255)                                                                                     tAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTc                                                >  2:1348312/1‑140 (MQ=255)                                                                                      aaTTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCg                                               >  1:428546/1‑140 (MQ=255)                                                                                      aaTTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCg                                               >  1:1441413/1‑140 (MQ=255)                                                                                          tCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGtat                                           >  1:448405/1‑140 (MQ=255)                                                                                             aTTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGtattat                                        >  2:174738/1‑140 (MQ=255)                                                                                                  aGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTg                                   <  1:1562524/140‑1 (MQ=255)                                                                                                    cGGTATTTATTGGTGTCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGc                                 >  1:849751/1‑140 (MQ=255)                                                                                                                 tgtCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTa                    >  1:1080444/1‑140 (MQ=255)                                                                                                                 tgtCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTa                    <  2:1270588/140‑1 (MQ=255)                                                                                                                   tCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtt                  >  2:95185/1‑140 (MQ=255)                                                                                                                   tCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtt                  >  1:221187/1‑140 (MQ=255)                                                                                                                   tCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtt                  <  2:75736/140‑1 (MQ=255)                                                                                                                   tCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtt                  >  2:1567654/1‑140 (MQ=255)                                                                                                                   tCATTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtt                  >  1:579401/1‑140 (MQ=255)                                                                                                                     aTTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtttt                <  1:207339/140‑1 (MQ=255)                                                                                                                     aTTATGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTAtttt                >  1:56033/1‑140 (MQ=255)                                                                                                                        aTGTCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTtaa             <  1:125537/140‑1 (MQ=255)                                                                                                                           tCGACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTtaataa          >  1:787306/1‑140 (MQ=255)                                                                                                                             gACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAAt        <  2:623345/140‑1 (MQ=255)                                                                                                                             gACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAAt        <  1:1348312/140‑1 (MQ=255)                                                                                                                             gACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAAt        <  2:368891/140‑1 (MQ=255)                                                                                                                             gACCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAAt        <  1:1134240/140‑1 (MQ=255)                                                                                                                              aCCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATg       >  2:435982/1‑140 (MQ=255)                                                                                                                              aCCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATg       >  1:307172/1‑140 (MQ=255)                                                                                                                              aCCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATg       >  2:1408717/1‑140 (MQ=255)                                                                                                                              aCCATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATg       >  2:1488587/1‑140 (MQ=255)                                                                                                                               ccATTATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATgg      >  2:407310/1‑140 (MQ=255)                                                                                                                                   tATGTGCCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGc  >  2:316726/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       CGGGGCTGGCTGCGGCCTTTAACGCGCCGCTGGCGGGTATTTTGTTTATTATCGAAGAGATGCGTCCGCAGTTTCGCTATACGTTAATTTCGATTAAAGCGGTATTTATTGGTGTCATTATGTCGACCATTATGTACCGGATTTTTAATCATGAAGTTGCGTTGATTGACGTCGGTAAACTTTCTGATGCGCCGCTTAATACGCTGTGGCTTTATCTGATCCTCGGTATTATTTTTGGCATTTTCGGCCCTATTTTTAATAAATGGGTGC  >  NC_000913/175657‑175926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑