Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,235,592	G→A	G110R (GGG→AGG)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,235,592	0	G	A	100.0%	292.6 / NA	83	G110R (GGG→AGG)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/44);  total (39/44)

GTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAG  >  NC_000913/2235457‑2235726                                                                                                                                        |                                                                                                                                       gTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGa                                                                                                                                    >  1:1529577/1‑140 (MQ=255)   gAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGaaa                                                                                                                                  >  1:47125/1‑140 (MQ=255)   gAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGaaa                                                                                                                                  <  2:775673/140‑1 (MQ=255)    aaaGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGaaaa                                                                                                                                 >  1:202412/1‑140 (MQ=255)    aaaGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGaaaa                                                                                                                                 >  2:600464/1‑140 (MQ=255)      agaagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATg                                                                                                                               >  1:82762/1‑140 (MQ=255)      agaagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATg                                                                                                                               >  2:1086080/1‑140 (MQ=255)      agaagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATg                                                                                                                               >  1:1088000/1‑140 (MQ=255)        aagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGag                                                                                                                             <  1:1318778/140‑1 (MQ=255)        aagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGag                                                                                                                             >  2:182228/1‑140 (MQ=255)        aagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGag                                                                                                                             <  1:243744/140‑1 (MQ=255)        aagaTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGag                                                                                                                             <  2:320132/140‑1 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  2:1138928/1‑140 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  2:1069339/1‑140 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  2:94390/1‑140 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  1:755143/1‑140 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  2:1385396/1‑140 (MQ=255)           aTACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGagcag                                                                                                                          >  1:533162/1‑140 (MQ=255)              ccGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCaa                                                                                                                       >  1:1090240/1‑140 (MQ=255)               cGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAAt                                                                                                                      >  1:1139908/1‑140 (MQ=255)               cGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAAt                                                                                                                      >  1:690584/1‑140 (MQ=255)                 gTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATgg                                                                                                                    >  2:1108231/1‑140 (MQ=255)                     tATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGggagg                                                                                                                >  2:207906/1‑140 (MQ=255)                      aTCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCTATCATGAGGGAAAATGAGCAGCAATGggagga                                                                                                               >  2:410428/1‑140 (MQ=255)                           cctCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTgg                                                                                                          <  1:1320302/138‑1 (MQ=255)                            ttCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGgc                                                                                                         <  1:600464/140‑1 (MQ=255)                              cAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGgcgc                                                                                                       <  1:294246/140‑1 (MQ=255)                                  aaTATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCt                                                                                                   <  1:450297/140‑1 (MQ=255)                                        gAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCa                                                                                             >  1:1246421/1‑140 (MQ=255)                                        gAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCa                                                                                             >  1:783635/1‑140 (MQ=255)                                           cAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCaaga                                                                                          >  2:712653/1‑140 (MQ=255)                                              aTTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGc                                                                                       >  1:1155846/1‑140 (MQ=255)                                                tGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGTTGCAAGAAGCtt                                                                                     >  2:1343299/1‑139 (MQ=255)                                                  cTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgc                                                                                   <  2:690584/140‑1 (MQ=255)                                                  cTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgc                                                                                   >  2:1103200/1‑140 (MQ=255)                                                  cTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgc                                                                                   <  2:47125/140‑1 (MQ=255)                                                  cTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgc                                                                                   <  2:1529577/140‑1 (MQ=255)                                                  cTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgc                                                                                   <  1:1108346/140‑1 (MQ=255)                                                   tGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGTGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgcg                                                                                  <  1:1460298/140‑1 (MQ=255)                                                    gAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGgcgc                                                                                 <  1:1395048/140‑1 (MQ=255)                                                      aCATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCgg                                                                               <  1:1138928/140‑1 (MQ=255)                                                        aTCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGat                                                                             <  2:697640/140‑1 (MQ=255)                                                         tCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCTATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGata                                                                            <  1:410428/140‑1 (MQ=255)                                                         tCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGata                                                                            <  2:213869/140‑1 (MQ=255)                                                          ccGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGatat                                                                           >  1:839425/1‑140 (MQ=255)                                                            gTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGa                                                                         >  2:395805/1‑140 (MQ=255)                                                             ttGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGAt                                                                        <  1:1052235/140‑1 (MQ=255)                                                             ttGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGAt                                                                        <  1:156224/140‑1 (MQ=255)                                                               ggAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCg                                                                      >  2:720383/1‑140 (MQ=255)                                                                  agagAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAgt                                                                   <  1:374070/140‑1 (MQ=255)                                                                        tCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACt                                                             >  1:26662/1‑140 (MQ=255)                                                                         cTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACtt                                                            <  2:297979/140‑1 (MQ=255)                                                                                cccTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGt                                                     <  1:207906/140‑1 (MQ=255)                                                                                     cGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCa                                                >  1:520998/1‑140 (MQ=255)                                                                                        cGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAAt                                             <  2:1090240/140‑1 (MQ=255)                                                                                           cTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGAc                                          <  2:839425/140‑1 (MQ=255)                                                                                               aggaagATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTtct                                      <  1:94390/140‑1 (MQ=255)                                                                                                    gATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGc                                 <  1:1385396/140‑1 (MQ=255)                                                                                                    gATTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCT‑GCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  2:202412/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  2:82762/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  2:1305914/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  2:755143/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                >  1:464951/1‑140 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  1:182228/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCg                                <  1:1069339/140‑1 (MQ=255)                                                                                                     aTTACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAACGACTTCTCATGCg                                <  2:1088000/140‑1 (MQ=255)                                                                                                       tACCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGAt                              <  1:1108231/140‑1 (MQ=255)                                                                                                        aCCCGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATg                             <  1:1198594/140‑1 (MQ=255)                                                                                                         cccGGACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATgg                            >  1:838827/1‑140 (MQ=255)                                                                                                             gACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                        <  2:1155846/140‑1 (MQ=255)                                                                                                             gACAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAg                        <  1:712653/140‑1 (MQ=255)                                                                                                               cAAAGTATTGATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCg                      <  2:273526/140‑1 (MQ=255)                                                                                                                        gATCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGc             >  2:590802/1‑140 (MQ=255)                                                                                                                         aTCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCggaca            <  2:26662/140‑4 (MQ=255)                                                                                                                         aTCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa            <  2:428818/140‑1 (MQ=255)                                                                                                                         aTCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa            <  2:1139908/140‑1 (MQ=255)                                                                                                                         aTCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCa            <  2:968276/140‑1 (MQ=255)                                                                                                                          tCGCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCaa           >  1:1333012/1‑140 (MQ=255)                                                                                                                            gCTTCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAg         >  1:836147/1‑140 (MQ=255)                                                                                                                              ttCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGcc       >  2:421932/1‑140 (MQ=255)                                                                                                                              ttCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGcc       >  2:333042/1‑140 (MQ=255)                                                                                                                              ttCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGcc       >  1:828632/1‑140 (MQ=255)                                                                                                                              ttCGATCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGACGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGcc       >  1:671601/1‑140 (MQ=255)                                                                                                                                   tCATGAGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAg  <  1:395805/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGCGCCTGGTGCAAGAAGCTGGCGCGGATATGATCGAGTGTAACTTCTCCTGTCCGCAAATGACTTCTCATGCGATGGGTAGCGATGTCGGGCAAAGCCCGGAG  >  NC_000913/2235457‑2235726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑