Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,251,690	T→C	V284A (GTA→GCA)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,251,690	0	T	C	100.0%	290.6 / NA	78	V284A (GTA→GCA)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (48/30);  total (48/30)

TATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAA  >  NC_000913/2251553‑2251823                                                                                                                                          |                                                                                                                                      tatCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAg                                                                                                                                     >  1:154594/1‑140 (MQ=255)    cGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCt                                                                                                                                  <  2:1278538/140‑1 (MQ=255)      gTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGa                                                                                                                                >  1:863516/1‑140 (MQ=255)      gTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGa                                                                                                                                >  1:751804/1‑140 (MQ=255)                gTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATaa                                                                                                                      <  1:1483763/140‑1 (MQ=255)                 ttCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAAc                                                                                                                     >  2:86265/1‑140 (MQ=255)                  tCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAAcc                                                                                                                    <  1:1145358/140‑1 (MQ=255)                   ccGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCg                                                                                                                   >  1:318759/1‑140 (MQ=255)                     gCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCggg                                                                                                                 <  2:706266/140‑1 (MQ=255)                      cTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGa                                                                                                                <  2:501193/140‑1 (MQ=255)                            cATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGga                                                                                                          <  1:1097571/140‑1 (MQ=255)                              tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGgaga                                                                                                        >  1:488119/1‑140 (MQ=255)                              tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGgaga                                                                                                        >  2:501283/1‑140 (MQ=255)                              tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGgaga                                                                                                        >  2:748467/1‑140 (MQ=255)                              tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGgaga                                                                                                        >  1:22043/1‑140 (MQ=255)                              tGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGgaga                                                                                                        >  1:1346780/1‑140 (MQ=255)                               gCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGAt                                                                                                       >  1:983476/1‑140 (MQ=255)                                   gacgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCtt                                                                                                   >  2:753071/1‑140 (MQ=255)                                     cgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGc                                                                                                 >  1:265599/1‑140 (MQ=255)                                     cgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGc                                                                                                 >  1:1294813/1‑140 (MQ=255)                                     cgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGc                                                                                                 >  2:835137/1‑140 (MQ=255)                                     cgacCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGc                                                                                                 >  1:557893/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:1492426/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  2:197831/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:974170/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:863751/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:1472048/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:1451781/1‑140 (MQ=255)                                        ccGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAt                                                                                              >  1:386021/1‑140 (MQ=255)                                           tttCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAAtttt                                                                                           <  1:394394/140‑1 (MQ=255)                                                   gCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTa                                                                                   <  2:856736/140‑1 (MQ=255)                                                    cATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTaa                                                                                  <  2:313466/140‑1 (MQ=255)                                                    cATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCCGATAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTaa                                                                                  >  2:1230198/1‑140 (MQ=255)                                                      ttCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAAcc                                                                                >  1:371966/1‑140 (MQ=255)                                                         cGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGc                                                                             <  2:1228776/140‑1 (MQ=255)                                                                 cTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTcag                                                                     <  2:863516/140‑1 (MQ=255)                                                                 cTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTcag                                                                     >  2:916370/1‑140 (MQ=255)                                                                 cTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCAGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTcag                                                                     <  2:691509/140‑1 (MQ=255)                                                                          aTCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGaa                                                            >  1:907740/1‑140 (MQ=255)                                                                          aTCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGaa                                                            >  2:398921/1‑140 (MQ=255)                                                                          aTCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGaa                                                            >  1:143097/1‑140 (MQ=255)                                                                           tCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGaaa                                                           <  2:1086132/140‑1 (MQ=255)                                                                           tCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGaaa                                                           >  1:784475/1‑140 (MQ=255)                                                                            cAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAAt                                                          >  2:658450/1‑140 (MQ=255)                                                                            cAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAAt                                                          <  1:753071/140‑1 (MQ=255)                                                                              aCCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTg                                                        <  2:431677/140‑1 (MQ=255)                                                                               cccGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGc                                                       <  2:265599/140‑1 (MQ=255)                                                                                 cGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCgg                                                     <  2:983476/140‑1 (MQ=255)                                                                                       tCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgc                                               <  2:1294813/140‑1 (MQ=255)                                                                                       tCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgc                                               <  2:488119/140‑1 (MQ=255)                                                                                         tGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgctg                                             <  1:197831/140‑1 (MQ=255)                                                                                          gggCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgctgc                                            >  1:1002500/1‑140 (MQ=255)                                                                                          gggCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgctgc                                            >  1:1567305/1‑140 (MQ=255)                                                                                          gggCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgctgc                                            >  2:1295008/1‑140 (MQ=255)                                                                                          gggCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACAtgctgc                                            >  1:746541/1‑140 (MQ=255)                                                                                           ggCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCa                                           <  2:1291275/140‑1 (MQ=255)                                                                                             cAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCaaa                                         >  2:576890/1‑140 (MQ=255)                                                                                             cAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCaaa                                         >  1:333326/1‑140 (MQ=255)                                                                                             cAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCaaa                                         >  2:715048/1‑140 (MQ=255)                                                                                             cAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCaaa                                         >  2:609376/1‑140 (MQ=255)                                                                                                     aTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGt                                 >  1:214278/1‑140 (MQ=255)                                                                                                     aTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGt                                 >  2:808143/1‑140 (MQ=255)                                                                                                     aTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGt                                 >  1:132099/1‑140 (MQ=255)                                                                                                     aTCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGt                                 >  1:1514775/1‑140 (MQ=255)                                                                                                        gCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTcg                              >  2:1074251/1‑140 (MQ=255)                                                                                                          cTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTcgcg                            <  2:371966/140‑1 (MQ=255)                                                                                                          cTGGCTGAAAGCGCAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTcgcg                            >  2:1054676/1‑140 (MQ=255)                                                                                                          cTGGCTGAAAGCGCAACAAACCGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTcgcg                            >  1:707678/1‑140 (MQ=255)                                                                                                                   aGCGCAACAAACTGAAACAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTgcggc                   <  2:1239007/140‑1 (MQ=255)                                                                                                                    gcgcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTgcggcg                  <  1:1230198/140‑1 (MQ=255)                                                                                                                    gcgcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTgcggcg                  <  2:751804/140‑1 (MQ=255)                                                                                                                     cgcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGgcgc                 <  2:1028275/140‑1 (MQ=255)                                                                                                                     cgcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGgcgc                 <  2:143097/140‑1 (MQ=255)                                                                                                                     cgcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGgcgc                 <  1:916370/140‑1 (MQ=255)                                                                                                                      gcAACAAACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCa                <  2:22043/140‑1 (MQ=255)                                                                                                                       caacaaACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCat               >  2:1469537/1‑140 (MQ=255)                                                                                                                       caacaaACTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCat               >  2:1449318/1‑140 (MQ=255)                                                                                                                           aaaCTGAAAAAGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCAtatta           <  2:557893/140‑1 (MQ=255)                                                                                                                                    aaGCGGCAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTaa  <  2:1526661/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TATCGGTCATGATGCGTTCCGCTGGATCATGCAGGACGACCGTTTCGACGGCATTCCGCTGATCCTCGAAACCATCAACCCGGATATCTGGGCAGAAGAGATCGCCTGGCTGAAAGCGCAACAAACTGAAAAAGCGGTAGCCTGAAGATGAATAACCGGGAAAAGGAGATCCTTGCAATTTTACGGCGTAACCCGCTGATTCAGCAGAACGAAATTGCGGACATGCTGCAAATCAGCCGTTCGCGCGTTGCGGCGCATATTATGGATTTAA  >  NC_000913/2251553‑2251823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑