Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,441,842	G→A	V27I (GTC→ATC)	mnmC →	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,441,842	0	G	A	100.0%	329.9 / NA	95	V27I (GTC→ATC)	mnmC	fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/34);  total (61/34)

CCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAA  >  NC_000913/2441712‑2441978                                                                                                                                   |                                                                                                                                         ccTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTAttttt                                                                                                                                 >  1:170810/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  1:671042/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  1:3958/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:1011569/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  1:914057/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:1411703/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:582401/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  1:719412/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:401933/1‑140 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       <  1:72635/140‑1 (MQ=255)           aTCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:16580/1‑140 (MQ=255)             cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACg                                                                                                                     >  1:919435/1‑140 (MQ=255)             cGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACg                                                                                                                     >  1:688437/1‑140 (MQ=255)                tAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGc                                                                                                                  >  2:201725/1‑140 (MQ=255)                tAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGc                                                                                                                  >  2:1376264/1‑140 (MQ=255)                       gAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAaga                                                                                                           >  2:594787/1‑140 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       <  1:1527694/127‑1 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATaa                                                                                                                       >  2:1527694/1‑127 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAagag                                                                                                          <  2:262842/140‑1 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAagag                                                                                                          >  1:1392838/1‑140 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAagag                                                                                                          >  1:1240278/1‑140 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAagag                                                                                                          >  2:1021931/1‑140 (MQ=255)                        aGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAagag                                                                                                          >  1:559809/1‑140 (MQ=255)                         gCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAgaga                                                                                                         <  1:600097/140‑1 (MQ=255)                             ccGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGAcgcg                                                                                                     <  2:767592/140‑1 (MQ=255)                              cGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGt                                                                                                    >  2:1010373/1‑140 (MQ=255)                                 cTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCACCCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTAt                                                                                                 <  1:1036136/140‑1 (MQ=255)                                      aGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGtttt                                                                                            >  2:64344/1‑140 (MQ=255)                                       gACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGttttt                                                                                           <  1:702686/140‑1 (MQ=255)                                         cGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCt                                                                                         <  2:1267111/140‑1 (MQ=255)                                          gAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTg                                                                                        >  1:1313706/1‑140 (MQ=255)                                                cTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGc                                                                                  <  2:1470111/140‑1 (MQ=255)                                                cTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGc                                                                                  <  1:1011569/140‑1 (MQ=255)                                                          acacTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTag                                                                        >  2:63968/1‑140 (MQ=255)                                                          acacTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTag                                                                        <  1:582401/140‑1 (MQ=255)                                                           cacTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTaga                                                                       >  1:465408/1‑140 (MQ=255)                                                             ctactCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAgg                                                                     >  2:1547361/1‑140 (MQ=255)                                                              tactCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGt                                                                    >  2:1523400/1‑140 (MQ=255)                                                              tactCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGt                                                                    >  2:451524/1‑140 (MQ=255)                                                               actCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTa                                                                   >  1:905881/1‑140 (MQ=255)                                                                 tCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACg                                                                 <  1:35291/140‑1 (MQ=255)                                                                 tCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACg                                                                 <  2:767957/140‑1 (MQ=255)                                                                   cATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCt                                                               >  1:631718/1‑140 (MQ=255)                                                                     tACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCttt                                                             >  2:844258/1‑140 (MQ=255)                                                                     tACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCttt                                                             >  2:948754/1‑140 (MQ=255)                                                                     tACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCttt                                                             >  2:969089/1‑140 (MQ=255)                                                                     tACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCttt                                                             >  2:306213/1‑140 (MQ=255)                                                                      aCAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTc                                                            >  2:48657/1‑140 (MQ=255)                                                                       cAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTcc                                                           <  1:975882/140‑1 (MQ=255)                                                                       cAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTcc                                                           <  1:594787/140‑1 (MQ=255)                                                                          ccTGCCAACCTCGAATTTAATGCTGAGGGTACGCCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                                        <  1:401933/140‑1 (MQ=255)                                                                          ccTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                                        >  2:659118/1‑140 (MQ=255)                                                                          ccTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                                        >  1:1083153/1‑140 (MQ=255)                                                                          ccTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                                        <  1:16580/140‑1 (MQ=255)                                                                          ccTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGa                                                        >  2:972166/1‑140 (MQ=255)                                                                           cTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTGTTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAg                                                       <  2:688437/140‑1 (MQ=255)                                                                           cTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAg                                                       <  2:671042/140‑1 (MQ=255)                                                                             gCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCa                                                     <  2:3958/140‑1 (MQ=255)                                                                             gCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCa                                                     >  2:244121/1‑140 (MQ=255)                                                                             gCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCa                                                     >  2:1250580/1‑140 (MQ=255)                                                                              ccAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCAt                                                    >  1:863581/1‑140 (MQ=255)                                                                               cAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACACGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATc                                                   >  2:606090/1‑140 (MQ=255)                                                                                  ccTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCcac                                                <  1:1379062/140‑1 (MQ=255)                                                                                   cTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCcaca                                               <  1:321642/140‑1 (MQ=255)                                                                                    tCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACAt                                              >  1:225648/1‑140 (MQ=255)                                                                                     cGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATc                                             >  2:228299/1‑140 (MQ=255)                                                                                       aaTTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct                                           <  2:1240278/140‑1 (MQ=255)                                                                                       aaTTTAATGCTGAGGGTACACCCGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCct                                           <  2:220802/140‑1 (MQ=255)                                                                                            aaTGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGtt                                      <  1:959322/140‑1 (MQ=255)                                                                                            aaTGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGtt                                      >  1:295595/1‑140 (MQ=255)                                                                                            aaTGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGtt                                      <  1:1010373/140‑1 (MQ=255)                                                                                                 tGAGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTgg                                 <  1:659118/140‑1 (MQ=255)                                                                                                   aGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTtg                                    <  2:97174/135‑1 (MQ=255)                                                                                                   aGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTtg                                    >  1:97174/1‑135 (MQ=255)                                                                                                   aGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTa                               >  2:378918/1‑140 (MQ=255)                                                                                                   aGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTa                               >  1:249842/1‑140 (MQ=255)                                                                                                   aGGGTACACCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTa                               >  2:732365/1‑140 (MQ=255)                                                                                                        acacCTGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACGAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCaga                          >  2:680264/1‑140 (MQ=255)                                                                                                             tGTTTCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCg                     <  1:201725/140‑1 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  1:583996/1‑140 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  1:865624/1‑140 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  2:134928/1‑140 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  1:1551466/1‑140 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  1:1404707/1‑140 (MQ=255)                                                                                                               tttCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGc                   >  2:926602/1‑140 (MQ=255)                                                                                                                 tCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCtt                 >  1:1305033/1‑140 (MQ=255)                                                                                                                 tCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCtt                 >  1:764947/1‑140 (MQ=255)                                                                                                                 tCCCGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCtt                 >  2:784585/1‑140 (MQ=255)                                                                                                                    cGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCgg              >  2:569366/1‑140 (MQ=255)                                                                                                                    cGAGATTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCgg              >  2:1423471/1‑140 (MQ=255)                                                                                                                      agaTTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCa            <  2:496463/140‑1 (MQ=255)                                                                                                                      agaTTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTCTGTGGTAGCAGAGAGCGGCTTCGGCa            <  1:868811/140‑1 (MQ=255)                                                                                                                       gaTTTTGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCAc           <  2:295595/140‑1 (MQ=255)                                                                                                                            tGACGATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGAt      <  1:378918/140‑1 (MQ=255)                                                                                                                                gATATCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTaaa  <  1:1180738/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CCTTACACACATCGCTAAGATCGAGCCACCGCCTGTAAGACGAGTAACTTACGTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGTCTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTGAGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAA  >  NC_000913/2441712‑2441978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑