Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,446,579	T→C	T299A (ACC→GCC)	aroC ←	chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,446,579	0	T	C	100.0%	292.9 / NA	79	T299A (ACC→GCC)	aroC	chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/25);  total (54/25)

GGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446444‑2446713                                                                                                                                         |                                                                                                                                       ggTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATg                                                                                                                                    >  2:1358311/1‑140 (MQ=255) ggTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGACCGTGACGGCCTTTGGCGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATg                                                                                                                                    >  1:1549698/1‑140 (MQ=255)  gTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGc                                                                                                                                   >  1:1397631/1‑140 (MQ=255)   tGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCt                                                                                                                                  >  1:962167/1‑140 (MQ=255)     aTCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTgg                                                                                                                                <  2:506501/140‑1 (MQ=255)       ccATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCTGTTAATGGTACG‑ACCCGGCACGGCAATACTGGAg                                                                                                                              >  1:507734/1‑140 (MQ=255)           tAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCg                                                                                                                          >  2:794951/1‑140 (MQ=255)            aaaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCgg                                                                                                                         >  2:1115443/1‑140 (MQ=255)             aaaCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGt                                                                                                                        >  2:1238076/1‑140 (MQ=255)               aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                      >  1:470440/1‑140 (MQ=255)               aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                      <  1:660298/140‑1 (MQ=255)               aCGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGttt                                                                                                                      >  1:1179663/1‑140 (MQ=255)                  aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   <  1:1543325/140‑1 (MQ=255)                  aTCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAg                                                                                                                   <  1:182059/140‑1 (MQ=255)                        aGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCat                                                                                                             <  2:363766/140‑1 (MQ=255)                             cGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATggg                                                                                                        <  1:949398/140‑1 (MQ=255)                               cTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCa                                                                                                      >  1:873174/1‑140 (MQ=255)                               cTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGACGGTTTCAGCGCCATATGGGCa                                                                                                      >  1:938306/1‑140 (MQ=255)                                 tCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAAt                                                                                                    >  1:663151/1‑140 (MQ=255)                                 tCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAAt                                                                                                    >  1:4166/1‑140 (MQ=255)                                   tGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGa                                                                                                  >  1:1420955/1‑140 (MQ=255)                                     cGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGAtt                                                                                                >  2:1477807/1‑140 (MQ=255)                                       aTCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtg                                                                                              >  2:17739/1‑140 (MQ=255)                                          ggCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctg                                                                                           >  1:366972/1‑140 (MQ=255)                                           gCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTtgctgc                                                                                          <  1:255376/140‑1 (MQ=255)                                             aCTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGTTCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGccc                                                                                        <  1:1028794/140‑1 (MQ=255)                                                gcgcGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgct                                                                                     >  1:1084704/1‑140 (MQ=255)                                                 cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  2:1257108/1‑140 (MQ=255)                                                 cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  1:1136849/1‑140 (MQ=255)                                                 cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  1:95984/1‑140 (MQ=255)                                                 cgcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctg                                                                                    >  1:1411991/1‑140 (MQ=255)                                                  gcgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGACGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctgc                                                                                   >  2:1449677/1‑140 (MQ=255)                                                   cgGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCgctgct                                                                                  >  1:1389706/1‑140 (MQ=255)                                                                aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  1:429985/1‑140 (MQ=255)                                                                aGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCgaga                                                                     >  2:1536577/1‑140 (MQ=255)                                                                  ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGTACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  1:8909/1‑140 (MQ=255)                                                                  ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:698803/1‑140 (MQ=255)                                                                  ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:142005/1‑140 (MQ=255)                                                                  ggATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:1067126/1‑140 (MQ=255)                                                                  ggATCGTGACGGCCTTTGGAGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGCACG‑ACCCGGCACGGCAATGCTGGAGGGCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAAt                                                                   >  2:1428567/1‑140 (MQ=255)                                                                     tCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                <  1:146625/140‑1 (MQ=255)                                                                     tCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgcc                                                                <  1:794951/140‑1 (MQ=255)                                                                      cGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAGTGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccg                                                               <  2:1155401/140‑1 (MQ=255)                                                                        tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                             >  1:613383/1‑140 (MQ=255)                                                                        tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                             >  2:1066912/1‑140 (MQ=255)                                                                        tGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGCCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATgccgcc                                                             >  1:1108202/1‑140 (MQ=255)                                                                            ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                         >  2:1100999/1‑140 (MQ=255)                                                                            ggCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCa                                                         >  1:646054/1‑140 (MQ=255)                                                                              ccTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATg                                                       >  1:921056/1‑140 (MQ=255)                                                                               cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                      <  1:1545779/140‑1 (MQ=255)                                                                               cTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATgg                                                      <  1:17739/140‑1 (MQ=255)                                                                                 tttgTGATCATTCAAACTTCTTCGCCAAGGCGGTTAGTCTTCCG‑ACCTGGCCCGGCAAGGTTTGGGGTCGTTTTCCGCGCCCTATGGTTTCTGATTTGCTCCCGGGTGCTGTTGCCGCCGAGAATGCCGCCCGCATGGtt                                                    <  2:1549698/137‑1 (MQ=255)                                                                                 ttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGtt                                                    <  2:366972/140‑1 (MQ=255)                                                                                 ttGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGtt                                                    >  2:325340/1‑140 (MQ=255)                                                                                  tGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTg                                                   >  1:654764/1‑140 (MQ=255)                                                                                   ggTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGc                                                  <  1:1477807/140‑1 (MQ=255)                                                                                     tGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGctc                                                <  1:1358311/140‑1 (MQ=255)                                                                                        tcatcTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTgg                                             >  1:1145793/1‑140 (MQ=255)                                                                                           tctcAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaaa                                          <  2:369117/140‑1 (MQ=255)                                                                                           tctcAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGaaa                                          <  2:95984/140‑1 (MQ=255)                                                                                            ctcAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                         >  1:4185/1‑140 (MQ=255)                                                                                            ctcAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAAc                                         >  1:517733/1‑140 (MQ=255)                                                                                              cAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCg                                       >  2:712724/1‑140 (MQ=255)                                                                                              cAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCg                                       <  2:4166/140‑1 (MQ=255)                                                                                               aaCTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGt                                      >  2:1462134/1‑140 (MQ=255)                                                                                                    cttcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTg                                 >  1:865729/1‑140 (MQ=255)                                                                                                      tcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  2:1586930/1‑140 (MQ=255)                                                                                                      tcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  1:1302000/1‑140 (MQ=255)                                                                                                      tcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  2:694449/1‑140 (MQ=255)                                                                                                      tcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               <  1:1115443/140‑1 (MQ=255)                                                                                                      tcGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGt                               >  1:97879/1‑140 (MQ=255)                                                                                                        cccAAGGCGGTTAGTGG‑ACGTCCCCGACGCGGCAATGTTGCAGTGGGGTTTAAGCGCTTTGTGGGAAATTTTTTTCTGCCCCTCGCAAATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGa                             <  2:507734/139‑1 (MQ=255)                                                                                                         ccAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGAt                            >  2:483121/1‑140 (MQ=255)                                                                                                         ccAAAGCGGTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGAt                            >  2:1179881/1‑140 (MQ=255)                                                                                                                ggTTAATGGTACG‑GCCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATGCCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  1:1257108/140‑1 (MQ=255)                                                                                                                ggTTAATGGTACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATcg                     <  2:532512/140‑1 (MQ=255)                                                                                                                         tACG‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTgg            >  1:1246296/1‑140 (MQ=255)                                                                                                                            g‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg         <  2:1179663/140‑1 (MQ=255)                                                                                                                            g‑ACCCGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTg         >  2:1250468/1‑140 (MQ=255)                                                                                                                               cccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGcc       >  2:210881/1‑140 (MQ=255)                                                                                                                                ccGGCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcg      <  2:4185/140‑1 (MQ=255)                                                                                                                                 cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc     >  1:794976/1‑140 (MQ=255)                                                                                                                                 cggcacggcaATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgc     >  2:559044/1‑140 (MQ=255)                                                                                                                                  ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg    >  2:483775/1‑140 (MQ=255)                                                                                                                                  ggCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg    <  2:27391/140‑1 (MQ=255)                                                                                                                                  ggCACGGCAATGCTGGAGGCCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAACCCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCcgcg    <  2:1108202/140‑1 (MQ=255)                                                                                                                                   gCACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCgcgc   >  1:693042/1‑140 (MQ=255)                                                                                                                                    cACGGCAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCa  <  1:1085345/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GGTGATCCATTAAAACGATCGCCAGCATCGCTTCTGCGATCGGCACTGCGCGGATCCCGACACAGGGATCGTGACGGCCTTTGGTGATCATCTCAACTTCTTCGCCAAAGCGGTTAATGGTACG‑ACCCGGCACGGTAATGCTGGAGGTCGGTTTCAGCGCCATATGGGCAATGATTTGCTGCCCGCTGCTGATACCGCCGAGAATGCCGCCCGCATGGTTGCTCTGGAAACCGTCTTTGGTGATTTCATCGCGGTTCTGGCTGCCGCGCA  >  NC_000913/2446444‑2446713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑