BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,639,916	A→G	intergenic (‑196/‑195)	uspB ← / → uspA	putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,639,916	0	A	G	99.3%	556.9 / NA	145	intergenic (‑196/‑195)	uspB/uspA	putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (96/48); minor base C (0/1); total (96/49)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

GCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGACGT  >  NC_000913/3639782‑3640051

 ccAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTg                                                                                                                                   >  2:508132/1‑140 (MQ=255)

 ccAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTg                                                                                                                                   <  1:48845/140‑1 (MQ=255)

        gTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCt                                                                                                                            <  2:870681/140‑1 (MQ=255)

         ttAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTa                                                                                                                           >  2:1573748/1‑140 (MQ=255)

                  ggTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAg                                                                                                                  >  1:665693/1‑140 (MQ=255)

                   gTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGc                                                                                                                 >  1:249125/1‑140 (MQ=255)

                       tGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAgg                                                                                                             >  2:675334/1‑140 (MQ=255)

                        ggAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGc                                                                                                            >  2:910292/1‑140 (MQ=255)

                             aaCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGAtt                                                                                                       >  1:168941/1‑140 (MQ=255)

                              aCCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTg                                                                                                      >  1:868141/1‑140 (MQ=255)

                                  cTTTTGTGGTGACCAACATCCGAGCGGCTCTATAGATAGTGTAGGCGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACgg                                                                                                  <  2:1093544/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  2:1241750/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       >  2:1541770/1‑140 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  1:671844/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  1:1573748/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  1:1565830/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  2:534474/140‑1 (MQ=255)

                                             aCCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGACAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGt                                                                                       <  1:1556546/140‑1 (MQ=255)

                                               cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCg                                                                                     >  2:1463878/1‑140 (MQ=255)

                                               cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCg                                                                                     <  1:149999/140‑1 (MQ=255)

                                               cAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCg                                                                                     <  2:586606/140‑1 (MQ=255)

                                                aaCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGc                                                                                    <  1:1268245/140‑1 (MQ=255)

                                                 aCATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCt                                                                                   <  1:389549/140‑1 (MQ=255)

                                                  cATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCta                                                                                  <  2:108722/140‑1 (MQ=255)

                                                   aTACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCtat                                                                                 <  2:196760/140‑1 (MQ=255)

                                                       gAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTAtaagg                                                                             >  2:1111525/1‑140 (MQ=255)

                                                            gCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTAtaaggtaagg                                                                        <  1:738455/140‑1 (MQ=255)

                                                              tctATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                                                                      <  1:1429665/140‑1 (MQ=255)

                                                              tctATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                                                                      <  2:549026/140‑1 (MQ=255)

                                                              tctATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGAt                                                                      <  1:1222679/140‑1 (MQ=255)

                                                                  tagatagTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTc                                                                  >  2:1008663/1‑140 (MQ=255)

                                                                    gatagTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCtt                                                                <  2:1281471/140‑1 (MQ=255)

                                                                       agTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAac                                                             >  2:568787/1‑140 (MQ=255)

                                                                       agTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAac                                                             >  2:293686/1‑140 (MQ=255)

                                                                       agTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAac                                                             >  1:1552972/1‑140 (MQ=255)

                                                                       agTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAac                                                             >  1:1341028/1‑140 (MQ=255)

                                                                       agTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAac                                                             >  1:8783/1‑140 (MQ=255)

                                                                        gtgtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                                                            >  1:73399/1‑140 (MQ=255)

                                                                        gtgtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAaca                                                            >  1:282948/1‑140 (MQ=255)

                                                                         tgtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAacac                                                           >  2:369484/1‑140 (MQ=255)

                                                                         tgtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAacac                                                           >  2:1217464/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  2:107940/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  2:195066/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  1:363369/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  1:175654/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  1:1220498/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  1:108764/1‑140 (MQ=255)

                                                                          gtAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACt                                                          >  1:1040916/1‑140 (MQ=255)

                                                                            aGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGa                                                        >  1:766701/1‑140 (MQ=255)

                                                                            aGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGa                                                        >  2:340675/1‑140 (MQ=255)

                                                                             ggAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGaa                                                       <  1:1541770/140‑1 (MQ=255)

                                                                              gagaTCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAAt                                                      >  1:1532664/1‑140 (MQ=255)

                                                                              gagaTCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAAt                                                      >  1:1388696/1‑140 (MQ=255)

                                                                               agaTCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATc                                                     >  1:941617/1‑140 (MQ=255)

                                                                                 aTCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCtt                                                   >  1:586391/1‑140 (MQ=255)

                                                                                  tCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCttt                                                  <  1:1008663/140‑1 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  2:660378/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  1:108810/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  1:534540/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  1:786852/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  1:830039/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  2:731944/1‑140 (MQ=255)

                                                                                   cAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTa                                                 >  2:1051718/1‑140 (MQ=255)

                                                                                    aGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTAc                                                >  1:47653/1‑140 (MQ=255)

                                                                                       ttgttTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGc                                             <  1:910292/140‑1 (MQ=255)

                                                                                       ttgttTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGc                                             <  1:402971/140‑1 (MQ=255)

                                                                                       ttgttTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGc                                             <  1:336738/140‑1 (MQ=255)

                                                                                       ttgttTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGc                                             <  2:1552972/140‑1 (MQ=255)

                                                                                        tgttTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCt                                            >  2:717100/1‑140 (MQ=255)

                                                                                                ttCCAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGc                                    >  1:581681/1‑140 (MQ=255)

                                                                                                  ccAGAAGGTTAACCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGccc                                  <  2:784243/140‑1 (MQ=255)

                                                                                                             aCCACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGGTAGCCCCGCGCACGTAg                       >  2:455177/1‑140 (MQ=255)

                                                                                                              ccACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGt                      <  1:568787/140‑1 (MQ=255)

                                                                                                               cACTATCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGtt                     <  2:363369/140‑1 (MQ=255)

                                                                                                                   aTCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCa                 >  1:930352/1‑140 (MQ=255)

                                                                                                                   aTCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCa                 >  2:599072/1‑140 (MQ=255)

                                                                                                                    tCAATATATTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAg                <  1:508132/140‑1 (MQ=255)

                                                                                                                       atatatTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGAc             >  1:820287/1‑140 (MQ=255)

                                                                                                                        tatatTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGAcg            >  2:1578092/1‑140 (MQ=255)

                                                                                                                         atatTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGAcgc           <  2:1388696/140‑1 (MQ=255)

                                                                                                                          tatTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGAcgcg          >  2:1142660/1‑140 (MQ=255)

                                                                                                                           atTCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCgg         >  2:1398119/1‑140 (MQ=255)

                                                                                                                            ttCATGTCGAGGATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        >  1:775777/1‑140 (MQ=255)

                                                                                                                            ttCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        >  2:650652/1‑140 (MQ=255)

                                                                                                                            ttCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        >  1:1033035/1‑140 (MQ=255)

                                                                                                                            ttCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        >  2:1385172/1‑140 (MQ=255)

                                                                                                                            ttCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        <  2:108810/140‑1 (MQ=255)

                                                                                                                            ttCATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCggg        >  1:891503/1‑140 (MQ=255)

                                                                                                                              cATGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTg      >  2:1472978/1‑140 (MQ=255)

                                                                                                                               aTGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGa     <  2:941617/140‑1 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:970973/1‑140 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:965482/1‑140 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:926560/1‑140 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:816741/1‑140 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:1464514/1‑140 (MQ=255)

                                                                                                                                tGTCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGAc    >  1:1364867/1‑140 (MQ=255)

                                                                                                                                  tCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCTCGCACGTAGTTCGGAGGGCACCGGTGACgg  >  2:432992/1‑139 (MQ=255)

                                                                                                                                  tCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGACGt  >  2:1330626/1‑140 (MQ=255)

                                                                                                                                  tCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGACGt  >  2:880510/1‑140 (MQ=255)

                                                                                                                                  tCGAGAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGGAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACCTAGGTCGCAGGACGCGGGGGACgg  >  2:505055/1‑139 (MQ=255)

GCCAATCAGTTAACACCAGGTCCTGGAGAAACCGCTTTTGTGGTGACCAACATACGAGCGGCTCTATAGATAGTGTAGGAGATCAGGTTGTTTTTTTTCCAGAAGGTTAACCACTATCAATATATTCATGTCGAAAATTTGTTTATCTAACGAGTAAGCAAGGCGGATTGACGGATCATCCGGGTCGCTATAAGGTAAGGATGGTCTTAACACTGAATCTTTACGGCTGGGTTAGCCCCGCGCACGTAGTTCGCAGGACGCGGGTGACGT  >  NC_000913/3639782‑3640051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`