BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,877,977	G→A	P715S (CCG→TCG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,877,977	0	G	A	99.2%	429.7 / ‑4.6	128	P715S (CCG→TCG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (57/69); total (57/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

Only 100 of 131 total aligned reads displayed.

GACTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGGCTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACC  >  NC_000913/3877842‑3878112

gacTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTga                                                                                                                                     <  1:592577/140‑1 (MQ=255)

 acTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgac                                                                                                                                    <  1:1279002/140‑1 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  1:376158/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  1:1053422/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  1:534460/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  2:752346/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  1:1513289/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  2:648961/1‑140 (MQ=255)

  cTTTCCGGGTCCATAGTGGTTTCCAACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTgacg                                                                                                                                   >  2:1163770/1‑140 (MQ=255)

     tCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGacgacg                                                                                                                                <  1:802579/140‑1 (MQ=255)

     tCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGacgacg                                                                                                                                <  2:1270911/140‑1 (MQ=255)

       cGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCt                                                                                                                              >  2:1532843/1‑140 (MQ=255)

             cATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCAc                                                                                                                        >  1:1196838/1‑140 (MQ=255)

              aTAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACg                                                                                                                       <  2:782138/140‑1 (MQ=255)

                   ggTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGa                                                                                                                  >  1:1374130/1‑140 (MQ=255)

                   ggTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGa                                                                                                                  >  1:1069652/1‑140 (MQ=255)

                    gTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGAt                                                                                                                 <  1:827637/140‑1 (MQ=255)

                       tCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATaaa                                                                                                              >  2:1448510/1‑140 (MQ=255)

                        cccACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGCCGGGACTCTTTCCCCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAAc                                                                                                             <  2:508672/140‑1 (MQ=255)

                         ccACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACg                                                                                                            <  1:520174/140‑1 (MQ=255)

                           acaGCTGTCCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGTTACCGACTGACGACGCTCGCCACGTTCGATAAACGCa                                                                                                          <  1:93187/140‑1 (MQ=255)

                            caGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCAt                                                                                                         <  1:1455747/140‑1 (MQ=255)

                            caGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCAt                                                                                                         <  2:171929/140‑1 (MQ=255)

                              gCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCAtct                                                                                                       >  1:1382075/1‑140 (MQ=255)

                              gCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCAtct                                                                                                       >  1:895116/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGGAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  2:1558287/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  2:1169898/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  1:898808/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  1:488962/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  2:1509671/1‑140 (MQ=255)

                                  ttCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATcttctt                                                                                                   >  1:140374/1‑140 (MQ=255)

                                   tCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCttcttc                                                                                                  >  1:153815/1‑140 (MQ=255)

                                   tCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCttcttc                                                                                                  >  2:1410496/1‑140 (MQ=255)

                                         ttCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCaa                                                                                            >  1:1279562/1‑140 (MQ=255)

                                            aTCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGcc                                                                                         >  1:298042/1‑140 (MQ=255)

                                            aTCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGcc                                                                                         >  2:1520368/1‑140 (MQ=255)

                                            aTCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGcc                                                                                         >  2:859122/1‑140 (MQ=255)

                                              ctcGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCAc                                                                                       >  1:308443/1‑140 (MQ=255)

                                               tcGCCCAGGCCTTTATAACGCTGGATGGAGAGGCCGCGACGGGTCTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCACCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACg                                                                                      <  2:733047/140‑1 (MQ=255)

                                                 gCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCa                                                                                    <  2:1513289/140‑1 (MQ=255)

                                                 gCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCa                                                                                    <  2:647091/140‑1 (MQ=255)

                                                  cccAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAg                                                                                   >  1:1555728/1‑140 (MQ=255)

                                                       aCCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTtct                                                                              >  2:171979/1‑140 (MQ=255)

                                                       aCCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTtct                                                                              >  1:1186042/1‑140 (MQ=255)

                                                           ttATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCAcc                                                                          >  1:1024521/1‑140 (MQ=255)

                                                            tataACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCAccc                                                                         <  1:556112/140‑1 (MQ=255)

                                                             ataACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCa                                                                        <  2:1024976/140‑1 (MQ=255)

                                                             ataACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCa                                                                        <  2:1053422/140‑1 (MQ=255)

                                                                 cGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGATGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGt                                                                    >  2:438620/1‑140 (MQ=255)

                                                                 cGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGt                                                                    <  1:753427/140‑1 (MQ=255)

                                                                  gCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGATGCTGGCTAACGACTGACGACGCTCGCTACGTTCGATAAACGCATCTTCTTCCATAAAGCCACGCAGTTTCTCACCCAGCGTg                                                                   >  2:84088/1‑140 (MQ=255)

                                                                   cTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGc                                                                  >  1:1501774/1‑140 (MQ=255)

                                                                    tggatggaGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCa                                                                 <  1:1155380/140‑1 (MQ=255)

                                                                      gatggaGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGa                                                               >  1:1587131/1‑140 (MQ=255)

                                                                        tggaGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATa                                                             <  1:1520368/140‑1 (MQ=255)

                                                                           agagGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGGCTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATacga                                                          <  2:1321927/140‑1 (MQ=255)

                                                                           agagGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATacga                                                          >  1:1013987/1‑140 (MQ=255)

                                                                           agagGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATacga                                                          <  1:176351/140‑1 (MQ=255)

                                                                            gagGCCGCGACGGGACTCTTTCA‑CAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATacgacg                                                        <  2:1308138/140‑1 (MQ=255)

                                                                             agGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATacgacg                                                        <  1:1506616/140‑1 (MQ=255)

                                                                                ccGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGata                                                     >  2:559501/1‑140 (MQ=255)

                                                                                ccGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGata                                                     >  1:321386/1‑140 (MQ=255)

                                                                                ccGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGata                                                     >  1:336844/1‑140 (MQ=255)

                                                                                     aCGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGc                                                >  2:530206/1‑140 (MQ=255)

                                                                                        ggACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCAc                                             <  1:648961/140‑1 (MQ=255)

                                                                                        ggACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCAc                                             <  1:1163770/140‑1 (MQ=255)

                                                                                        ggACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCAc                                             <  2:1501774/140‑1 (MQ=255)

                                                                                        ggACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCAc                                             <  1:687331/140‑1 (MQ=255)

                                                                                         gACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCAcc                                            <  1:752346/140‑1 (MQ=255)

                                                                                           ctctTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCgg                                          <  2:298042/140‑1 (MQ=255)

                                                                                              tttCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGa                                       >  2:615479/1‑140 (MQ=255)

                                                                                               ttCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGAt                                      <  1:394663/140‑1 (MQ=255)

                                                                                               ttCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGAt                                      <  2:877680/140‑1 (MQ=255)

                                                                                                tCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCGGATACGACGATATTCGCCACCGGTGATa                                     <  1:1329841/140‑1 (MQ=255)

                                                                                                tCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATa                                     <  2:898808/140‑1 (MQ=255)

                                                                                                 cACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATaa                                    <  2:1435014/140‑1 (MQ=255)

                                                                                                 cACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATaa                                    <  1:316612/140‑1 (MQ=255)

                                                                                                   ccagccagTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAAc                                  <  2:534460/140‑1 (MQ=255)

                                                                                                      gccagTCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCTACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCg                               >  2:327512/1‑140 (MQ=255)

                                                                                                           tCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATc                          <  2:1196838/140‑1 (MQ=255)

                                                                                                           tCCAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGGGATAAACTCGTGATc                          >  2:1459200/1‑140 (MQ=255)

                                                                                                            ccAGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATcc                         <  2:1069652/140‑1 (MQ=255)

                                                                                                              aGCGCCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAg                       <  2:1382075/140‑1 (MQ=255)

                                                                                                                cgcCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCg                     >  1:866348/1‑140 (MQ=255)

                                                                                                                cgcCTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCg                     >  1:74804/1‑140 (MQ=255)

                                                                                                                  ccTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGa                   <  2:54977/140‑1 (MQ=255)

                                                                                                                  ccTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGa                   <  2:397983/140‑1 (MQ=255)

                                                                                                                  ccTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGa                   <  1:1160507/140‑1 (MQ=255)

                                                                                                                  ccTGCTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGa                   <  2:1576545/140‑1 (MQ=255)

                                                                                                                      cTCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGt               <  2:308443/140‑1 (MQ=255)

                                                                                                                       tCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTc              <  1:559501/140‑1 (MQ=255)

                                                                                                                       tCGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTc              <  2:1385067/140‑1 (MQ=255)

                                                                                                                        cGAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCa             <  1:730654/140‑1 (MQ=255)

                                                                                                                         gAAGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAg            <  2:1186042/140‑1 (MQ=255)

                                                                                                                          aaGCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGt           <  1:1253141/140‑1 (MQ=255)

                                                                                                                            gCTGGCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTAt         <  1:1532843/140‑1 (MQ=255)

                                                                                                                               ggCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCca      <  2:153815/140‑1 (MQ=255)

                                                                                                                               ggCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCca      >  2:905224/1‑140 (MQ=255)

                                                                                                                                gCTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCcac     >  1:753025/1‑140 (MQ=255)

                                                                                                                                 cTACCGACTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCcaca    <  2:909326/140‑1 (MQ=255)

GACTTTCCGGGTCCATAGTGGTTTCCCACAGCTGTTCCGGGTTCATCTCGCCCAGACCTTTATAACGCTGGATGGAGAGGCCGCGACGGGACTCTTTCACCAGCCAGTCCAGCGCCTGCTCGAAGCTGGCTACCGGCTGACGACGCTCGCCACGTTCGATAAACGCATCTTCTTCCAGCAAGCCACGCAGTTTCTCACCCAGCGTGCAGATACGACGATATTCGCCACCGGTGATAAACTCGTGATCCAGCGGATAGTCAGTATCCACACC  >  NC_000913/3877842‑3878112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`