Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,172,137	(A)7→6	coding (75/333 nt)	ygiZ ←	conserved inner membrane protein YgiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,172,131	0	A	.	100.0%	355.6 / NA	80	coding (81/333 nt)	ygiZ	conserved inner membrane protein YgiZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (30/50);  total (30/50)

AAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGAT  >  NC_000913/3171994‑3172264                                                                                                                                          |                                                                                                                                      aagataagaATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCaaa                                                                                                                                     >  1:266961/1‑140 (MQ=255)      aagaATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCaaaaaaga                                                                                                                                <  1:850206/140‑3 (MQ=255)            aataaGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTAt                                                                                                                         <  2:390305/140‑1 (MQ=255)             ataaGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTAtt                                                                                                                        >  2:169266/1‑140 (MQ=255)              taaGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGGTTGCAACATGCTATCTTCCCCTGAAACGGAAGGCATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATCCTTATTGAAAGC‑AAAAAAGAATCTATTa                                                                                                                       <  2:848884/140‑1 (MQ=255)                  tGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCa                                                                                                                   <  2:942859/140‑1 (MQ=255)                  tGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCa                                                                                                                   >  1:80722/1‑140 (MQ=255)                   ggCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCaa                                                                                                                  >  2:200888/1‑140 (MQ=255)                   ggCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCaa                                                                                                                  >  2:337836/1‑140 (MQ=255)                   ggCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCaa                                                                                                                  >  1:521136/1‑140 (MQ=255)                     cGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAta                                                                                                                >  2:688661/1‑140 (MQ=255)                     cGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAta                                                                                                                >  1:589495/1‑140 (MQ=255)                     cGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAta                                                                                                                >  1:725029/1‑140 (MQ=255)                        aagaagAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagt                                                                                                             <  2:367642/140‑1 (MQ=255)                        aagaagAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagt                                                                                                             <  2:501265/140‑1 (MQ=255)                        aagaagAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagt                                                                                                             <  1:899033/140‑1 (MQ=255)                        aagaagAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagt                                                                                                             <  1:142625/140‑1 (MQ=255)                        aagaagAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagt                                                                                                             <  2:165767/140‑1 (MQ=255)                          gaagaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAAtaagtaa                                                                                                           <  2:531992/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  2:810542/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  1:952995/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  2:718150/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  1:633677/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  2:655452/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  1:195170/140‑1 (MQ=255)                             gaaAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTACTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCa                                                                                                        <  2:644362/140‑1 (MQ=255)                              aaaaaaTGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCAt                                                                                                       >  1:411930/1‑140 (MQ=255)                              aaaaaaTGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGCATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCAt                                                                                                       >  1:94975/1‑140 (MQ=255)                                     gctgcAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATtatatat                                                                                                >  1:235022/1‑140 (MQ=255)                                         cAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCa                                                                                            <  1:278545/140‑1 (MQ=255)                                         cAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATAGATCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCa                                                                                            >  2:235063/1‑140 (MQ=255)                                          aaTCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCaa                                                                                           <  1:178393/140‑1 (MQ=255)                                           aTCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAAt                                                                                          <  1:572811/140‑1 (MQ=255)                                                   ttgcAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCaaa                                                                                  >  1:753964/1‑140 (MQ=255)                                                      cAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAatat                                                                               >  1:758781/1‑140 (MQ=255)                                                           taCGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGttt                                                                          <  1:688661/140‑1 (MQ=255)                                                                 cTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAAttt                                                                    <  2:139802/140‑1 (MQ=255)                                                                 cTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAAttt                                                                    <  2:919450/140‑1 (MQ=255)                                                                  ttCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAAtttt                                                                   <  2:653312/140‑1 (MQ=255)                                                                  ttCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAAtttt                                                                   <  1:705783/140‑1 (MQ=255)                                                                   tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTc                                                                  >  1:1004041/1‑140 (MQ=255)                                                                   tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTc                                                                  <  2:521136/140‑1 (MQ=255)                                                                   tCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTc                                                                  >  1:620485/1‑140 (MQ=255)                                                                    cGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCt                                                                 >  2:746994/1‑140 (MQ=255)                                                                     gCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTg                                                                >  1:649957/1‑140 (MQ=255)                                                                       cTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCt                                                              <  1:163637/140‑1 (MQ=255)                                                                       cTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCt                                                              <  1:409806/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  2:825950/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  2:235022/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  2:266961/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  1:169266/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  1:861736/140‑1 (MQ=255)                                                                        tGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCtt                                                             <  1:637053/140‑1 (MQ=255)                                                                          aaaCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTa                                                           >  2:614513/1‑140 (MQ=255)                                                                          aaaCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTa                                                           <  2:589495/140‑1 (MQ=255)                                                                          aaaCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTa                                                           >  1:84440/1‑140 (MQ=255)                                                                            aCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                         <  1:389204/140‑1 (MQ=255)                                                                            aCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                         <  2:784146/140‑1 (MQ=255)                                                                            aCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                         <  1:906865/140‑1 (MQ=255)                                                                            aCGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAAc                                                         <  2:519504/140‑1 (MQ=255)                                                                             cGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACa                                                        <  2:317168/140‑1 (MQ=255)                                                                                      taCTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGt                                               >  2:648910/1‑140 (MQ=255)                                                                                      taCTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGt                                               >  2:930471/1‑140 (MQ=255)                                                                                      taCTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGt                                               >  2:755570/1‑140 (MQ=255)                                                                                        cTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTAc                                             >  1:889091/1‑140 (MQ=255)                                                                                              atatTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCAt                                       >  1:877183/1‑140 (MQ=255)                                                                                                    cctccCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATGTATCAGTACGCTCATAAAAAt                                 <  2:114622/140‑1 (MQ=255)                                                                                                    cctccCGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAAt                                 <  1:45210/140‑1 (MQ=255)                                                                                                        ccGACTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCAt                             <  1:930471/140‑1 (MQ=255)                                                                                                           aCTCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTta                          <  2:101389/140‑1 (MQ=255)                                                                                                             tCAAGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTtata                        <  2:725029/140‑1 (MQ=255)                                                                                                                aGCATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAAtt                     <  2:84440/140‑1 (MQ=255)                                                                                                                  cATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTAt                   <  1:614513/140‑1 (MQ=255)                                                                                                                  cATCCAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTAt                   <  2:649957/140‑1 (MQ=255)                                                                                                                      cAGTCATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAAc               >  2:780665/1‑140 (MQ=255)                                                                                                                          cATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGa           <  2:94975/140‑1 (MQ=255)                                                                                                                          cATACTTATTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGa           <  2:620485/140‑1 (MQ=255)                                                                                                                                 aTTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACg       <  2:722243/137‑1 (MQ=255)                                                                                                                                 aTTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTg    >  2:420568/1‑140 (MQ=255)                                                                                                                                 aTTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTg    >  2:322979/1‑140 (MQ=255)                                                                                                                                 aTTGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATGATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACg       >  1:722243/1‑137 (MQ=255)                                                                                                                                  ttGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGa   <  2:889091/140‑1 (MQ=255)                                                                                                                                   tGAAAGC‑AAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGAt  >  1:127107/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      AAGATAAGAATAATAAGTGGCGTAAGAAGAAAAAATGCTGCAATCATTGCTTGCAATATACGATCTTCGCCTGAAACGGAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATACTTATTGAAAGCAAAAAAAGAATCTATTAACCAATAAGTAAGCATTATATATAGCAATGCTTCAAATATTGTTTTAATTTTCTGCTTTAACATATATCAGTACGCTCATAAAAATCCATTTATAATTATCAACATGACACGTTGAT  >  NC_000913/3171994‑3172264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑