Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,089,445	(G)5→4	coding (413/2019 nt)	pgaB ←	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,089,441	0	G	.	100.0%	192.1 / NA	44	coding (417/2019 nt)	pgaB	poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (22/22);  total (22/22)

ATGTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACG  >  NC_000913/1089302‑1089575                                                                                                                                            |                                                                                                                                       aTGTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACg                                                                                                                                        <  2:421186/140‑1 (MQ=255)   gTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACggg                                                                                                                                      <  2:944676/140‑1 (MQ=255)   gTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACggg                                                                                                                                      <  1:859705/140‑1 (MQ=255)   gTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACggg                                                                                                                                      <  2:617262/140‑1 (MQ=255)   gTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACggg                                                                                                                                      <  1:480796/140‑1 (MQ=255)        agaAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACggggccca                                                                                                                                 <  1:247323/140‑5 (MQ=255)        agaAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGACGACggggccca                                                                                                                                 <  2:860563/140‑5 (MQ=255)            gCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAca                                                                                                                            >  2:166120/1‑140 (MQ=255)               aGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagca                                                                                                                         <  1:347668/140‑1 (MQ=255)               aGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagca                                                                                                                         >  2:670732/1‑140 (MQ=255)               aGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagca                                                                                                                         <  2:478568/140‑1 (MQ=255)               aGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagca                                                                                                                         <  1:75547/140‑1 (MQ=255)               aGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagca                                                                                                                         <  2:1038656/140‑1 (MQ=255)                gCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATAcagcag                                                                                                                        >  1:332497/1‑140 (MQ=255)                    aaCGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCa                                                                                                                    <  1:935278/140‑1 (MQ=255)                                aCGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTg                                                                                                        <  1:494845/140‑1 (MQ=255)                                aCGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTg                                                                                                        <  1:691483/140‑1 (MQ=255)                                aCGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTg                                                                                                        <  1:800310/140‑1 (MQ=255)                                   cgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGTCTgaag                                                                                                     <  1:190360/140‑1 (MQ=255)                                   cgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTgaag                                                                                                     >  2:296755/1‑140 (MQ=255)                                   cgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTgaag                                                                                                     >  1:151590/1‑140 (MQ=255)                                   cgcAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTgaag                                                                                                     >  1:873822/1‑140 (MQ=255)                                       aCTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAAtt                                                                                                 <  1:1039796/140‑1 (MQ=255)                                        cTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTg                                                                                                >  2:734148/1‑140 (MQ=255)                                                aCTTGTTGCCACGTGGCTAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCGCTGGAAGGCCTGAAGAATTGGGAAGAcg                                                                                        <  2:1006111/140‑1 (MQ=255)                                                aCTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcg                                                                                        <  2:517612/140‑1 (MQ=255)                                                aCTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcg                                                                                        <  1:151202/140‑1 (MQ=255)                                                aCTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcg                                                                                        <  2:726252/140‑1 (MQ=255)                                                aCTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcg                                                                                        <  2:522489/140‑1 (MQ=255)                                                  tacctgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGAcgcg                                                                                      <  2:808832/136‑1 (MQ=255)                                                    gttgCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCggg                                                                                    <  2:409599/140‑1 (MQ=255)                                                          aCGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATaaa                                                                              <  1:138731/140‑1 (MQ=255)                                                           cGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATaaaa                                                                             <  1:996266/140‑1 (MQ=255)                                                              ggCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAAct                                                                          >  2:162005/1‑140 (MQ=255)                                                                        tctcGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCg                                                                >  1:717489/1‑140 (MQ=255)                                                                         ctcGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGt                                                               <  2:497258/140‑1 (MQ=255)                                                                            gatcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGtcat                                                            >  2:930048/1‑140 (MQ=255)                                                                             atcgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGtcatc                                                           >  2:693140/1‑140 (MQ=255)                                                                               cgaCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCaa                                                         >  1:526034/1‑140 (MQ=255)                                                                                 aCCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCaaaa                                                       >  2:466494/1‑140 (MQ=255)                                                                                   cAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGt                                                     <  2:267389/140‑1 (MQ=255)                                                                                          tCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACt                                              >  2:442121/1‑140 (MQ=255)                                                                                           cGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTa                                             >  2:691800/1‑140 (MQ=255)                                                                                           cGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTa                                             >  2:298804/1‑140 (MQ=255)                                                                                                          gTTTATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTa                              >  2:189448/1‑140 (MQ=255)                                                                                                            ttATCCGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGc                            >  2:811707/1‑140 (MQ=255)                                                                                                                 cGCTGGCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGttt                       >  2:902467/1‑140 (MQ=255)                                                                                                                     ggCGTATCGACCCAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGATTTtcct                   >  2:1009736/1‑140 (MQ=255)                                                                                                                               cccAACTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGAtgtg         <  2:489342/140‑1 (MQ=255)                                                                                                                                   aCTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTc     >  2:899824/1‑140 (MQ=255)                                                                                                                                   aCTGCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTc     >  2:326276/1‑140 (MQ=255)                                                                                                                                      gCCGAC‑GGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACg  <  2:717489/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                       ATGTATGAGAAGCGAGCTCAACGAGCCGGGAACGCGCAACTTCTCGCACTTGTTGCCACGTGGCAAAATATTCTCGATCGACCAACTCATCGCCAAATTTTACTTGTTTATCCGCTGGCGTATCGACCCAACTGCCGACGGGGGCCCATACAGCAGGCCACTGGAAGGCCTGAAGAATTGGGAAGACGCGGGTATAAAAACTCTGGTAGCCGTCATCAAAAGTCAGCACTACAGCTTTTTCCGGTAGCGGTTTTCCTCCTCGATGTGCTTCACG  >  NC_000913/1089302‑1089575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑