Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,521,565	(A)6→7	coding (456/1350 nt)	yjhF ←	KpLE2 phage‑like element; putative transporter YjhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,521,559	1	.	A	100.0%	190.1 / NA	56	L154F (TTG→TTT)	yjhF	KpLE2 phage‑like element; putative transporter YjhF
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (47/9);  total (47/9)

CAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑AAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGA  >  NC_000913/4521431‑4521692                                                                                                                                  |                                                                                                                                      cAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑aaaaaaaccgt                                                                                                                            <  1:772308/140‑6 (MQ=255)    aaTTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGtgtg                                                                                                                          <  1:362659/140‑1 (MQ=255)      tttATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCg                                                                                                                        >  1:974438/1‑140 (MQ=255)          ttCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCa                                                                                                                    >  2:802235/1‑140 (MQ=255)                    ggCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGa                                                                                                          >  2:531131/1‑140 (MQ=255)                    ggCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGa                                                                                                          >  2:289892/1‑140 (MQ=255)                       cGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAg                                                                                                       >  1:302238/1‑140 (MQ=255)                         cAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCa                                                                                                     >  1:14928/1‑140 (MQ=255)                         cAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCa                                                                                                     >  1:190851/1‑140 (MQ=255)                             gAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCgg                                                                                                 <  1:484720/140‑1 (MQ=255)                                tAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGcac                                                                                              >  2:452029/1‑140 (MQ=255)                                 aaaGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGcaca                                                                                             <  1:47202/140‑1 (MQ=255)                                        ggAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATaa                                                                                      >  2:856808/1‑140 (MQ=255)                                        ggAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATaa                                                                                      >  2:562043/1‑140 (MQ=255)                                               cGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCgg                                                                               >  1:879141/1‑140 (MQ=255)                                                 aCAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTg                                                                             >  1:139722/1‑140 (MQ=255)                                                           cGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGc                                                                   >  2:878675/1‑140 (MQ=255)                                                             tataTCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCtt                                                                 >  2:899655/1‑140 (MQ=255)                                                             tataTCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCtt                                                                 >  2:899656/1‑140 (MQ=255)                                                                atCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCAc                                                              <  1:289892/140‑1 (MQ=255)                                                                    gTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGc                                                          >  2:710248/1‑140 (MQ=255)                                                                     tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  1:650049/1‑140 (MQ=255)                                                                     tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  2:411685/1‑140 (MQ=255)                                                                     tACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCa                                                         >  1:44501/1‑140 (MQ=255)                                                                          aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  2:168941/1‑140 (MQ=255)                                                                          aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  1:807998/1‑140 (MQ=255)                                                                          aTCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGc                                                    >  2:487360/1‑140 (MQ=255)                                                                            ccTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGa                                                  >  1:192427/1‑140 (MQ=255)                                                                            ccTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGACAATAGCGa                                                  >  2:471866/1‑140 (MQ=255)                                                                               aCATCAGCGCCATATTCCCTGGCGATAACAGTCGTGCCGGGGGGCGGCGGCAAAAAAAACGTGTGCGACCAGCAAACCAGAAAGCATCGGCGCACACATAAACATCGGCGATATTTTTGCTTCACGGGCAATAGCGAATa                                               >  2:26074/1‑140 (MQ=255)                                                                               aCATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATa                                               <  1:802235/140‑1 (MQ=255)                                                                                  tCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATaaaa                                            >  2:694530/1‑140 (MQ=255)                                                                                   cAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAAt                                           >  2:554098/1‑140 (MQ=255)                                                                                     gcgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAg                                         >  1:513081/1‑140 (MQ=255)                                                                                     gcgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAg                                         >  1:116431/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:119269/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  2:91826/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:193995/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  2:228090/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:454312/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:757839/1‑140 (MQ=255)                                                                                      cgcCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAgg                                        >  1:700443/1‑140 (MQ=255)                                                                                        ccATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTa                                      >  1:440816/1‑140 (MQ=255)                                                                                            atTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCag                                  <  2:757839/140‑1 (MQ=255)                                                                                                   ggCGATAACAGTCTGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACAAATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:465461/1‑140 (MQ=255)                                                                                                   ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:418016/1‑140 (MQ=255)                                                                                                   ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  2:580427/1‑140 (MQ=255)                                                                                                   ggCGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAAtt                           >  1:606035/1‑140 (MQ=255)                                                                                                     cGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTaa                         >  1:170765/1‑140 (MQ=255)                                                                                                     cGATAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAAGATTaa                         >  2:750424/1‑140 (MQ=255)                                                                                                       aTAACAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAAc                       >  2:360340/1‑140 (MQ=255)                                                                                                         aaCAGTCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCg                     >  2:406541/1‑140 (MQ=255)                                                                                                              tCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTc                >  1:394457/1‑140 (MQ=255)                                                                                                              tCGGGCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGGACCAGAAGAATTAAACCGACTTc                >  1:172448/1‑140 (MQ=255)                                                                                                                gggCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGa              >  1:847275/1‑140 (MQ=255)                                                                                                                gggCCGGGGTGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGa              >  1:985097/1‑140 (MQ=255)                                                                                                                         tGCGGCGGCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTg     <  2:974438/140‑1 (MQ=255)                                                                                                                           cggcggCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCg   <  1:899655/140‑1 (MQ=255)                                                                                                                           cggcggCAAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCg   <  1:899656/140‑1 (MQ=255)                                                                                                                            ggcggcaAAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGa  >  2:45030/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      CAGAATTTATTCAATACCGGGCCGCAAAGAATAAAGGTTGGAATGCCGACAATGATACCGTATATCAGTACCAATCCTACATCAGCGCCATATTCCCTGGCGATAACAGTCGGGCCGGGGTGCGGCGGC‑AAAAAACCGTGTGCGACCAGCAAACCAGAAAGCATCGGCACACACATAAACATCGGTGATATTTTTGCTTCACGGGCAATAGCGAATAAAATAGGTACCAGAAGAATTAAACCGACTTCGAAAAAAAGTGCGA  >  NC_000913/4521431‑4521692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑