Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT32.5% 58.2 / 18.2 40intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/25);  new base T (13/0);  total (15/25)
Fisher's exact test for biased strand distribution p-value = 8.73e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT  >  NC_000913/3206296‑3206572
                                                                                                                                            |                                                                                                                                         
gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTc                                                                                                                                           <  2:905309/140‑1 (MQ=255)
    aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGa                                                                                                                                       >  2:625694/1‑140 (MQ=255)
              ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGCGTTTTTTCAGACAAATAaaaa                                                                                                                             >  2:268545/1‑140 (MQ=255)
                cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGACAaaaaaaaaaa                                                                                                                           >  2:258947/1‑140 (MQ=255)
                 ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAAc                                                                                                                          <  1:448550/140‑1 (MQ=255)
                     ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                      <  1:458028/140‑1 (MQ=255)
                      tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                     <  1:524407/140‑1 (MQ=255)
                          gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGc                                                                                                                 >  2:817055/1‑140 (MQ=255)
                           cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGcc                                                                                                                <  2:712753/140‑1 (MQ=255)
                               atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAAAAGAGGTGCCatga                                                                                                            >  2:11618/1‑140 (MQ=255)
                                   tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAg                                                                                                        <  2:931978/140‑1 (MQ=255)
                                    cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                       <  2:1034377/140‑1 (MQ=255)
                                    cattcTTATTTTCGCGTTGATTCACGAACTGTAAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                       <  1:901590/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                     <  2:60075/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                     <  2:439423/140‑1 (MQ=255)
                                      ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                     <  1:481013/140‑1 (MQ=255)
                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGATGCCCTGATGAGCGGAAga                                                                                                 >  2:855169/1‑139 (MQ=255)
                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGGGAAAga                                                                                                 >  1:921418/1‑139 (MQ=255)
                                          tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑GGAATTTTTTCCGACAAATACAAAAAAAGGAGGTGCCCTGATGAGGGAAAga                                                                                                 >  2:340954/1‑139 (MQ=255)
                                            ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGACAAAAACAAAAAAAGGAGGTGGCATGATGAGGgaaagaaa                                                                                               >  2:68159/1‑140 (MQ=255)
                                             tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                              <  2:120351/140‑1 (MQ=255)
                                              ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAATAAAAAAAAAGGAGGTGCCCTGATGAGCGAAAGtaata                                                                                             >  1:88217/1‑140 (MQ=255)
                                                   gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                        <  1:625694/140‑1 (MQ=255)
                                                       aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                    <  1:214071/140‑1 (MQ=255)
                                                            cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTg                                                                               <  2:754600/140‑1 (MQ=255)
                                                                 tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAAAAAACAACAAGCAGTGAaaaa                                                                          >  1:339009/1‑140 (MQ=255)
                                                                   ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGAAAGGAATAAGCAACAAGCAGTGAATaaat                                                                        >  1:202232/1‑138 (MQ=255)
                                                                   ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCTGATGAGGGAAAGGAATAAACAAAAAGCAGTGAaaaaag                                                                        >  2:531453/1‑138 (MQ=255)
                                                                            tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGTAACAGGCAGTGAATAAGTTGACAgaga                                                               <  2:539423/140‑1 (MQ=255)
                                                                             atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGGGGAAGAAATAAACAAAAAGCAGGGAAAAAGGTGACAGAGAt                                                              >  2:103471/1‑140 (MQ=255)
                                                                                     tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGGAATTTTTCCGAAAAAAAAAAAAAAATGAGGTTCCCA‑CATGGGCGAAAAGAATAAAGAAAAAGGACTGAAAAAATTGACAGAGATTTTCGCtc                                                      >  2:740397/1‑139 (MQ=255)
                                                                                       gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGAAAGAAATAAACAACAAGCAGGGAATAAATTGACAGAGATTGTCGCTaaa                                                    >  2:837809/1‑139 (MQ=255)
                                                                                        cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTGGCCTGATGGGGGGAAGTAAAAAAAAACAGGCAGAGAAAAAAGTGACAGAGATTGTTGCTaaat                                                   >  2:841168/1‑138 (MQ=255)
                                                                                            aaaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACT‑GAGTTGCCATGAGGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                              <  1:1013132/140‑1 (MQ=255)
                                                                                                 taAACCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCa                                          <  2:878726/140‑1 (MQ=255)
                                                                                                 taAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                          <  1:15849/140‑1 (MQ=255)
                                                                                                 taAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑AGAAATTTTTCTGACAAAAAAAAAAAATTGAGGTGTCATGATGAGCGGAAATAAAAAAAAAAAAGCAGGGAAGAAGTTTGAAGAGAATGTCGCTTACTTTTGCGccc                                          >  2:607876/1‑139 (MQ=255)
                                                                                                     aCCACATTTTTAGTGGTAGTTTGTGGCGG‑TGATTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGAt                                      <  2:204212/140‑1 (MQ=255)
                                                                                                                aGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                           <  1:202531/140‑1 (MQ=255)
                                                                                                                aGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                           <  1:817055/140‑1 (MQ=255)
                                                                                                                         ttGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                  <  1:837809/140‑1 (MQ=255)
                                                                                                                           gtgGCGG‑TGAATTTTTCCAGACAAATAAAAAACCTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGAc                <  1:620975/140‑1 (MQ=255)
                                                                                                                            tgGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGGGAAAGTAAAAAACAACAAGCAGTGAATAAATTGACAGAGAATGTCGCTAACTTTACCGCCCTGATTTTTTCCCCCATGCCTGATGACg               >  2:798916/1‑140 (MQ=255)
                                                                                                                              gCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg             <  2:202232/140‑1 (MQ=255)
                                                                                                                                    gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa        <  1:798916/140‑1 (MQ=255)
                                                                                                                                    gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa        <  1:835601/140‑1 (MQ=255)
                                                                                                                                          ttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt  >  1:771661/1‑140 (MQ=255)
                                                                                                                                            |                                                                                                                                         
GCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACT  >  NC_000913/3206296‑3206572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑