Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,891	C→T	R167R (CGC→CGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,891	0	C	T	100.0%	145.4 / NA	43	R167R (CGC→CGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/24);  total (19/24)

GAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTT  >  NC_000913/22772‑23026                                                                                                                        |                                                                                                                                        gAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCgg                                                                                                                     <  2:808239/140‑1 (MQ=255) gAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCgg                                                                                                                     <  1:353105/140‑1 (MQ=255)   aTACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCa                                                                                                                   <  1:600121/140‑1 (MQ=255)    tACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCaa                                                                                                                  <  1:555552/140‑1 (MQ=255)     aCGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAAc                                                                                                                 >  1:575131/1‑140 (MQ=255)      cgcgGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACg                                                                                                                <  2:206789/140‑1 (MQ=255)      cgcgGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACg                                                                                                                <  1:349171/140‑1 (MQ=255)        cggcgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAAACATCGGCAACGGt                                                                                                              <  2:36657/140‑1 (MQ=255)         ggcgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTc                                                                                                             <  2:984143/140‑1 (MQ=255)         ggcgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTc                                                                                                             <  1:608837/140‑1 (MQ=255)           cgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCAc                                                                                                           >  2:639888/1‑140 (MQ=255)           cgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCAc                                                                                                           <  2:268737/140‑1 (MQ=255)           cgACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCAc                                                                                                           <  1:707411/140‑1 (MQ=255)            gACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCAcc                                                                                                          >  1:29306/1‑140 (MQ=255)             aCCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCt                                                                                                         >  1:426330/1‑140 (MQ=255)                    ttGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCCCCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACaaa                                                                                                  <  2:804509/140‑1 (MQ=255)                    ttGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACaaa                                                                                                  <  1:662620/140‑1 (MQ=255)                         ggTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGgcgc                                                                                             >  1:160022/1‑140 (MQ=255)                            cAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGaa                                                                                          <  2:408958/140‑1 (MQ=255)                              aCGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGc                                                                                        <  1:709069/140‑1 (MQ=255)                               cGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGcc                                                                                       >  1:684589/1‑140 (MQ=255)                                   aaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGtt                                                                                   >  1:823007/1‑140 (MQ=255)                                   aaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGtt                                                                                   <  2:291730/140‑1 (MQ=255)                                         ttATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTgg                                                                             >  1:107295/1‑140 (MQ=255)                                          tATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGt                                                                            >  2:744281/1‑140 (MQ=255)                                                tCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTg                                                                      >  2:858530/1‑140 (MQ=255)                                                    ggCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTg                                                                  <  2:575131/140‑1 (MQ=255)                                                      cGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGcc                                                                >  1:840176/1‑140 (MQ=255)                                                                   tGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGgc                                                   >  2:482891/1‑140 (MQ=255)                                                                      tCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGgcgga                                                <  2:277333/140‑1 (MQ=255)                                                                       cGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaa                                               >  1:23736/1‑140 (MQ=255)                                                                         cACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagc                                             <  2:574397/140‑1 (MQ=255)                                                                         cACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGgcggaagc                                             <  2:967846/140‑1 (MQ=255)                                                                              gTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGcggaagcggaag                                        <  1:434518/140‑1 (MQ=255)                                                                               tACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGt                                       >  1:615737/1‑140 (MQ=255)                                                                               tACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGt                                       >  2:318974/1‑140 (MQ=255)                                                                                    gACCATGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGt                                  >  2:822596/1‑140 (MQ=255)                                                                                         tGGACTTCAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTAc                             >  1:595231/1‑140 (MQ=255)                                                                                                cAAAACTGAAGCCAACATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACaaaa                      >  2:993514/1‑140 (MQ=255)                                                                                                             aaCATCATCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCAt         <  2:545505/140‑1 (MQ=255)                                                                                                               catcatCCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCg       <  2:29306/140‑1 (MQ=255)                                                                                                                  catcCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACg    <  2:840176/140‑1 (MQ=255)                                                                                                                    tcCGTGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGtt  >  1:351986/1‑140 (MQ=255)                                                                                                                        |                                                                                                                                        GAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTT  >  NC_000913/22772‑23026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑