Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,070,755	G→A	R164C (CGC→TGC)	fbaA ←	fructose‑bisphosphate aldolase class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,070,755	0	G	A	100.0%	189.9 / NA	57	R164C (CGC→TGC)	fbaA	fructose‑bisphosphate aldolase class II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/21);  total (36/21)

TCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAC  >  NC_000913/3070619‑3070891                                                                                                                                         |                                                                                                                                         tCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTc                                                                                                                                       <  1:140631/140‑1 (MQ=255)               aTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGc                                                                                                                         <  1:727925/140‑1 (MQ=255)               aTCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGc                                                                                                                         >  2:326451/1‑140 (MQ=255)                   aCGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGAt                                                                                                                     >  1:1006757/1‑140 (MQ=255)                        ttCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGa                                                                                                                <  2:5375/140‑1 (MQ=255)                          ccGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATg                                                                                                              <  1:813532/140‑1 (MQ=255)                          ccGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATg                                                                                                              <  1:373647/140‑1 (MQ=255)                                 ggTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCtt                                                                                                       >  1:1001637/1‑140 (MQ=255)                                       cAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCagag                                                                                                 <  1:210626/140‑1 (MQ=255)                                        aGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAgaga                                                                                                <  1:466593/140‑1 (MQ=255)                                          tGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAtt                                                                                              >  2:227031/1‑140 (MQ=255)                                          tGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAtt                                                                                              >  2:599760/1‑140 (MQ=255)                                           gCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttc                                                                                             >  2:843172/1‑140 (MQ=255)                                           gCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttc                                                                                             >  1:66765/1‑140 (MQ=255)                                            cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:217020/1‑140 (MQ=255)                                            cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:180857/1‑140 (MQ=255)                                            cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  1:983707/1‑140 (MQ=255)                                            cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  1:970044/1‑140 (MQ=255)                                            cAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGAttct                                                                                            >  2:727659/1‑140 (MQ=255)                                                    tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:78292/1‑140 (MQ=255)                                                    tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:188903/1‑140 (MQ=255)                                                    tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:218209/1‑140 (MQ=255)                                                    tCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAg                                                                                    >  1:952762/1‑140 (MQ=255)                                                         gtgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTA‑TTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAt                                                                              <  2:453696/140‑1 (MQ=255)                                                          tgGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGAt                                                                              <  1:775015/140‑1 (MQ=255)                                                               gttgtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCAtgt                                                                         >  1:78831/1‑140 (MQ=255)                                                                  gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  1:944554/1‑140 (MQ=255)                                                                  gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  1:317340/1‑140 (MQ=255)                                                                  gtCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGAATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTgag                                                                      >  2:331084/1‑140 (MQ=255)                                                                                    accaccGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTAc                                                    <  1:180857/140‑1 (MQ=255)                                                                                       accGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCgg                                                 <  2:970044/140‑1 (MQ=255)                                                                                             gCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTg                                           >  2:605324/1‑140 (MQ=255)                                                                                                aCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGa                                        >  1:17247/1‑140 (MQ=255)                                                                                                aCCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGa                                        >  2:709138/1‑140 (MQ=255)                                                                                                  ccAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAg                                      <  2:186760/140‑1 (MQ=255)                                                                                                  ccAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAg                                      <  2:95536/140‑1 (MQ=255)                                                                                                   cAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAgt                                     >  2:755821/1‑140 (MQ=255)                                                                                                    aGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAgtg                                    <  1:599760/140‑1 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:652353/1‑140 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:37350/1‑140 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:442191/1‑140 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  2:936670/1‑140 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:435550/1‑140 (MQ=255)                                                                                                      ttCGATTTCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGtt                                  >  1:418443/1‑140 (MQ=255)                                                                                                            ttCCAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCAc                            <  1:612313/140‑1 (MQ=255)                                                                                                              ccAGAGTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCAccc                          <  2:78292/140‑1 (MQ=255)                                                                                                                   gTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTc                     >  1:220065/1‑140 (MQ=255)                                                                                                                   gTCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTc                     <  2:66765/140‑1 (MQ=255)                                                                                                                    tCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTcc                    >  2:636075/1‑140 (MQ=255)                                                                                                                    tCATGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTcc                    >  2:208038/1‑140 (MQ=255)                                                                                                                       tGCCGATTTTGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAAc                 >  2:705225/1‑140 (MQ=255)                                                                                                                              tttGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGt          <  1:331084/140‑1 (MQ=255)                                                                                                                              tttGGACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGt          <  2:1006757/140‑1 (MQ=255)                                                                                                                                  gACATGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGAt      >  2:109143/1‑140 (MQ=255)                                                                                                                                      tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  <  2:571164/140‑1 (MQ=255)                                                                                                                                      tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  >  1:847652/1‑140 (MQ=255)                                                                                                                                      tGCACTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAc  <  2:17247/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TCGGTGTATGCGTAATCAACGTCTTCCGGCTGGGTGTACAGTGCAGAAGCGTCCATGTGGCTGTTGTCCACGCCGTCTTCTTCACCACCGGTGCAACCCAGTTCGATTTCCAGAGTCATGCCGATTTTGGACATGCGCTCCAGGTATTTAGAGCAGATTTCGATGTTCTCTTGCAGAGATTCTTCAGACAGGTCGATCATGTGAGAAGAGAACAGCGGCTTACCGGTAGCTGCGAAGTGTTTTTCACCCGCGTCCAACAGACCGTCGATCCAC  >  NC_000913/3070619‑3070891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑