Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,080,055	T→C	E527E (GAA→GAG)	tktA ←	transketolase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,080,055	0	T	C	100.0%	177.6 / NA	48	E527E (GAA→GAG)	tktA	transketolase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/32);  total (16/32)

CAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCA  >  NC_000913/3079919‑3080191                                                                                                                                         |                                                                                                                                         cagtcagtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTcc                                                                                                                                       <  2:1019467/140‑1 (MQ=255)    tcagtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCctgc                                                                                                                                    <  1:71224/140‑1 (MQ=255)      agtTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCctgctg                                                                                                                                  <  1:492021/140‑1 (MQ=255)         ttttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTgcgc                                                                                                                               <  1:261059/140‑1 (MQ=255)          tttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGcc                                                                                                                              <  1:849819/140‑1 (MQ=255)          tttCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGcc                                                                                                                              <  2:896730/140‑1 (MQ=255)               tAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGGGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGtt                                                                                                                         <  2:788043/140‑1 (MQ=255)                 ggCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCt                                                                                                                       <  1:254804/140‑1 (MQ=255)                 ggCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCt                                                                                                                       <  2:329876/140‑1 (MQ=255)                  gcagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      >  2:345324/1‑140 (MQ=255)                   cagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGa                                                                                                                     <  2:866186/140‑1 (MQ=255)                   cagcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGa                                                                                                                     <  2:47476/140‑1 (MQ=255)                    agcaACAGGCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCTGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGAc                                                                                                                    <  2:227136/140‑1 (MQ=255)                    agcaACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGAc                                                                                                                    <  1:400819/140‑1 (MQ=255)                      caACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACgg                                                                                                                  <  1:140132/140‑1 (MQ=255)                      caACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACgg                                                                                                                  >  1:151439/1‑140 (MQ=255)                            ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAgg                                                                                                            <  2:1011548/140‑1 (MQ=255)                            ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGATGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      >  2:937209/1‑130 (MQ=255)                            ccAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGATGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTg                                                                                                                      <  1:937209/130‑1 (MQ=255)                                           aaCCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCGGTGCGGTCggg                                                                                             >  2:435298/1‑140 (MQ=255)                                                gTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTc                                                                                        >  2:1030255/1‑140 (MQ=255)                                                      aTGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACg                                                                                  <  1:849499/140‑1 (MQ=255)                                                            aTCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAac                                                                            <  2:859361/140‑1 (MQ=255)                                                             tCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAaca                                                                           <  2:151439/140‑1 (MQ=255)                                                                    cGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCGCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTAtt                                                                    >  2:774221/1‑140 (MQ=255)                                                                      gCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTc                                                                  >  2:965675/1‑140 (MQ=255)                                                                          aCCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCAcg                                                              <  2:91575/140‑1 (MQ=255)                                                                                     cTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGAt                                                   <  2:140082/140‑1 (MQ=255)                                                                                      tttCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGAtt                                                  <  2:145650/140‑1 (MQ=255)                                                                                               aTAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGt                                         <  1:220213/140‑1 (MQ=255)                                                                                                tAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGGTCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTc                                        >  2:581642/1‑140 (MQ=255)                                                                                                    caccGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCacac                                    <  1:463151/140‑1 (MQ=255)                                                                                                     accGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACg                                   <  1:477834/140‑1 (MQ=255)                                                                                                      ccGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACgg                                  >  1:542580/1‑140 (MQ=255)                                                                                                         cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:676452/1‑140 (MQ=255)                                                                                                         cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  2:856758/1‑140 (MQ=255)                                                                                                         cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               <  1:861619/140‑1 (MQ=255)                                                                                                         cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:368705/1‑140 (MQ=255)                                                                                                         cgcgcgATGTTTGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACg                               >  1:212685/1‑140 (MQ=255)                                                                                                                   ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  1:345324/140‑1 (MQ=255)                                                                                                                   ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  2:358951/140‑1 (MQ=255)                                                                                                                   ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  2:264712/140‑1 (MQ=255)                                                                                                                   ttGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGAc                     <  1:1030255/140‑1 (MQ=255)                                                                                                                    tGCCAGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACa                    >  1:692984/1‑140 (MQ=255)                                                                                                                        aGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGtt                <  1:446923/140‑1 (MQ=255)                                                                                                                        aGTTGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGtt                <  2:218389/140‑1 (MQ=255)                                                                                                                          ttGCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCg              >  2:267519/1‑140 (MQ=255)                                                                                                                            gCTCTTCAGTTCGCTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCggg            >  2:947190/1‑140 (MQ=255)                                                                                                                                    gttcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTAcgcg    >  2:585605/1‑140 (MQ=255)                                                                                                                                    gttcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTAcgcg    >  2:456668/1‑140 (MQ=255)                                                                                                                                      tcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCa  <  1:976369/140‑1 (MQ=255)                                                                                                                                      tcgctcCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCa  <  1:856758/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAATCAGTTCCGGCTGACCGGCGCAGTCTTTCAGCACATAACCACCGCGCGCGATGTTTGCCAGTTGCTCTTCAGTTCGTTCCTGCTGCGCCAGGTTCTGACGGGAGAGGATCAGTGCGGTCGGGCCGTCCTGACGCTCAACACCGTATTTCCACGCGACCGCGGATTCAACCTGGTCACACGGACGCCATGTAGACATGTTCGGGGTTACGCGCA  >  NC_000913/3079919‑3080191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑