Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,174,743	A→G	G218G (GGT→GGC)	parE ←	DNA topoisomerase IV subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,174,743	0	A	G	100.0%	102.9 / NA	28	G218G (GGT→GGC)	parE	DNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

CACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGT  >  NC_000913/3174616‑3174873                                                                                                                                |                                                                                                                                   cACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAg                                                                                                                        <  2:226440/140‑1 (MQ=255)    cGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGCAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGCCCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCAc                                                                                                                     <  2:1023846/140‑1 (MQ=255)    cGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCAc                                                                                                                     <  2:750752/140‑1 (MQ=255)         tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                <  2:52614/140‑1 (MQ=255)         tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                <  2:730836/140‑1 (MQ=255)         tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                >  2:328217/1‑140 (MQ=255)         tCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCg                                                                                                                >  2:122345/1‑140 (MQ=255)            ggCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCgttg                                                                                                             <  2:691363/140‑1 (MQ=255)                   acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      >  2:64900/1‑140 (MQ=255)                   acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:800942/140‑1 (MQ=255)                   acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:442431/140‑1 (MQ=255)                   acaGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTa                                                                                                      <  2:474236/140‑1 (MQ=255)                      gTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTGCCGATAAACGGTATTTCCGGCAGCGTCGGCAGACCTTTTACCGCGTCCGCCATGTAATCATACAGGCCGACCTGATAGCACCAGCGTTGTTCGGTAttg                                                                                                   <  2:700063/140‑1 (MQ=255)                        aGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGTGTTGTTCGGTAttgtt                                                                                                 >  2:486105/1‑140 (MQ=255)                           gcCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGAGAAACGGATATTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCAAGGAAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGAt                                                                                              >  2:486054/1‑140 (MQ=255)                                 tCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATc                                                                                        >  1:925638/1‑140 (MQ=255)                                 tCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATc                                                                                        >  2:142605/1‑140 (MQ=255)                                       gCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTaaa                                                                                  >  1:913462/1‑140 (MQ=255)                                           cAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTg                                                                              >  2:562911/1‑140 (MQ=255)                                                      gCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGcc                                                                   >  1:1021358/1‑140 (MQ=255)                                                          aaTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAggg                                                               <  2:621249/140‑1 (MQ=255)                                                            ttACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGca                                                             >  1:757441/1‑140 (MQ=255)                                                                cGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAAt                                                         >  1:607305/1‑140 (MQ=255)                                                                 gATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATa                                                        >  2:349147/1‑140 (MQ=255)                                                                         gTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTg                                                <  1:898622/140‑1 (MQ=255)                                                                                   cAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGca                                      <  2:871350/140‑1 (MQ=255)                                                                                                                   ggTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg      <  2:524692/140‑1 (MQ=255)                                                                                                                   ggTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCggg      <  1:495010/140‑1 (MQ=255)                                                                                                                    ttAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGc     >  1:175583/2‑140 (MQ=255)                                                                                                                    gTAATCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGc     >  2:529288/1‑140 (MQ=255)                                                                                                                       aTCATTCAGGCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGt  >  1:549393/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                   CACCGCCTTCCGGCAGCCACAGTAGCGCCCAGTCCACAGCTTCAGTATCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGT  >  NC_000913/3174616‑3174873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑