Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,052	A→G	V521A (GTG→GCG)	yhbX ←	putative hydrolase, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,052	0	A	G	100.0%	216.6 / NA	57	V521A (GTG→GCG)	yhbX	putative hydrolase, inner membrane
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/30);  total (27/30)

CTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAG  >  NC_000913/3319923‑3320187                                                                                                                                  |                                                                                                                                        ctcctcTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGaa                                                                                                                               <  1:686546/140‑1 (MQ=255)      ctGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAAcat                                                                                                                          >  1:127716/1‑140 (MQ=255)      ctGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAAcat                                                                                                                          >  1:422591/1‑140 (MQ=255)         ccGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAAcatcat                                                                                                                       >  2:456297/1‑140 (MQ=255)         ccGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAAcatcat                                                                                                                       >  2:439303/1‑140 (MQ=255)         ccGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAAcatcat                                                                                                                       >  2:22969/1‑140 (MQ=255)               cAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTg                                                                                                                 <  2:912352/140‑1 (MQ=255)                 ggCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCa                                                                                                               >  2:522412/1‑140 (MQ=255)                     tGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATTATCGAAAACATCATGGTTTGCATCca                                                                                                           <  2:833133/140‑1 (MQ=255)                       ccGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCcaca                                                                                                         >  1:362299/1‑140 (MQ=255)                             gTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGt                                                                                                   <  2:993416/140‑1 (MQ=255)                               ggAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGa                                                                                                 <  1:458476/140‑1 (MQ=255)                               ggAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGa                                                                                                 <  1:744735/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:127716/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  1:380760/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  1:946851/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:330647/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  1:380563/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  1:379605/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:515104/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:592883/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:863269/140‑1 (MQ=255)                                 agaATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGATACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGt                                                                                               <  2:3102/140‑1 (MQ=255)                                   aaTCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGtct                                                                                             <  2:653429/140‑1 (MQ=255)                                      cTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCt                                                                                          <  2:34599/140‑1 (MQ=255)                                       tGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGtct                                                                                             <  1:273698/136‑1 (MQ=255)                                       tGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGtct                                                                                             >  2:273698/1‑136 (MQ=255)                                       tGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCtt                                                                                         >  2:104434/1‑140 (MQ=255)                                                aaGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGtgtg                                                                                >  1:488847/1‑140 (MQ=255)                                                     cAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATc                                                                           <  1:966711/140‑1 (MQ=255)                                                       gTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTGTATAGTGTGCAATCAc                                                                         <  2:910789/140‑1 (MQ=255)                                                               aCATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCaa                                                                 <  1:527145/140‑1 (MQ=255)                                                                  tCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCg                                                              >  1:88186/1‑140 (MQ=255)                                                                              ttCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGtt                                                  >  1:272325/1‑140 (MQ=255)                                                                                cAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTcc                                                >  2:694393/1‑140 (MQ=255)                                                                                     gtggggtTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCtt                                           >  2:574133/1‑140 (MQ=255)                                                                                        gggtTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGt                                        >  1:297343/1‑140 (MQ=255)                                                                                                  gCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATc                              <  1:886983/140‑1 (MQ=255)                                                                                                  gCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATc                              <  1:592059/140‑1 (MQ=255)                                                                                                  gCTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATc                              <  1:715754/140‑1 (MQ=255)                                                                                                   cTTATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATcc                             >  2:1028574/1‑140 (MQ=255)                                                                                                    ttATCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCa                            <  2:422591/140‑1 (MQ=255)                                                                                                      aTCCTCTGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACg                          >  2:323745/1‑140 (MQ=255)                                                                                                         ctctGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCTGGCTTTGTTACCCCCATCCACGCAt                       >  2:904245/1‑140 (MQ=255)                                                                                                         ctctGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCAt                       >  2:296176/1‑140 (MQ=255)                                                                                                            tGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATtaa                    >  2:946222/1‑140 (MQ=255)                                                                                                            tGTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCAGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATtaa                    >  2:778748/1‑140 (MQ=255)                                                                                                             gTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATtaat                   >  2:800132/1‑140 (MQ=255)                                                                                                             gTAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATtaat                   <  2:381833/140‑1 (MQ=255)                                                                                                              tAAACTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATtaata                  >  2:1037762/1‑140 (MQ=255)                                                                                                                 aCTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAgg               >  1:881531/1‑140 (MQ=255)                                                                                                                  cTCCTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGt              <  1:78837/140‑1 (MQ=255)                                                                                                                    ccTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTaa            <  1:439303/140‑1 (MQ=255)                                                                                                                     cTTTCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACTCATTAATAAGGTAat           >  1:87432/1‑140 (MQ=255)                                                                                                                      tttCTGAGCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAatt          >  2:658899/1‑140 (MQ=255)                                                                                                                             gCATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAAATATTGTaa   >  1:1015112/1‑140 (MQ=255)                                                                                                                              cATCGCATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAg  <  2:488847/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                        CTCCTCTGCCGCTTCAGGCGTGCCGCAGGTGGAGAATCTGGAAAACAAAGGCCAGTAATTCGACATCAACCCTGCCCTTCAGGGGTGGGGTTACCTTGCTTATCCTCTGTAAACTCCTTTCTGAGCATCACATAATCGAAAACATCATGGTTTGCATCCACAACCCGTGAGTCTCCTTTATAGTGTGCAATCACTTCCTCAAGCGTTTGCGGCTGTTCCGGCTTTGTTACCCCCATCCACGCATTAATAAGGTAATTATTGTAAG  >  NC_000913/3319923‑3320187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑