Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,424,742	T→C	noncoding (2042/2904 nt)	rrlD ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,424,742	0	T	C	100.0%	28.2 / NA	15	noncoding (2042/2904 nt)	rrlD	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/7);  total (8/7)

GGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGA  >  NC_000913/3424605‑3424791                                                                                                                                          |                                                  gggTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCac                                                 <  1:143806/140‑1 (MQ=17) gggTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCac                                                 <  1:6341/140‑1 (MQ=17)   gtggtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCacac                                               <  1:851270/140‑1 (MQ=17)      gtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGc                                            >  1:381136/1‑140 (MQ=18)      gtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGc                                            <  2:1002598/140‑1 (MQ=17)      gtATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGc                                            >  2:215218/1‑140 (MQ=18)       tATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCa                                           <  2:559535/140‑1 (MQ=17)         tttCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATc                                         >  2:427934/1‑140 (MQ=18)                 tCGGCTCCATGCAGACTGGCGTCCACACTTCTAAACCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGc                                 <  2:796430/140‑1 (MQ=12)                   ggCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGCGa                               >  2:717058/1‑140 (MQ=18)                               aCTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTACCGCGGGCACACTGCATCTTCACAGCGAGTTCAATTTCAc                   >  1:934121/1‑140 (MQ=18)                                cTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGCGAGTTCAATTTCACt                  <  2:337688/140‑1 (MQ=17)                                  ggCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGCGAGTTCAATTTCACTGa                >  1:414536/1‑140 (MQ=18)                                          cacTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCggg        >  2:960867/1‑140 (MQ=18)                                                tAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGCACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGgaga  >  2:806496/1‑140 (MQ=11)                                                                                                                                          |                                                  GGGTGGTATTTCAAGGTCGGCTCCATGCAGACTGGCGTCCACACTTCTAAGCCTCCCACCTATCCTACACATCAAGGCTCAATGTTCAGTGTCAAGCTATAGTAAAGGTTCACGGGGTCTTTCCGTCTTGCCGCGGGTACACTGCATCTTCACAGCGAGTTCAATTTCACTGAGTCTCGGGTGGAGA  >  NC_000913/3424605‑3424791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑