Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,483,979	A→G	S260G (AGC→GGC)	yheT →	putative hydrolase YheT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,483,979	0	A	G	100.0%	200.4 / NA	54	S260G (AGC→GGC)	yheT	putative hydrolase YheT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/28);  total (26/28)

CTGGCAGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACA  >  NC_000913/3483844‑3484115                                                                                                                                        |                                                                                                                                         cTGGCAGCCTTCCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGgcgc                                                                                                                                      <  1:394200/140‑1 (MQ=255)      aGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGc                                                                                                                                 <  2:858573/140‑1 (MQ=255)       gCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTGCGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGcc                                                                                                                                <  1:305916/140‑1 (MQ=255)           cccccGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATg                                                                                                                            <  2:333615/139‑1 (MQ=255)                  aaCGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAAcc                                                                                                                     >  2:840126/1‑140 (MQ=255)                      cTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGAt                                                                                                                 >  2:828151/1‑140 (MQ=255)                       tGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATc                                                                                                                >  2:37530/1‑140 (MQ=255)                       tGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATc                                                                                                                >  2:488662/1‑140 (MQ=255)                              tAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATGACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAAc                                                                                                         <  1:633376/140‑1 (MQ=255)                              tAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAAc                                                                                                         <  1:391558/140‑1 (MQ=255)                                      cgcAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTg                                                                                                 >  1:325059/1‑140 (MQ=255)                                            tAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGattatt                                                                                           <  2:218327/140‑1 (MQ=255)                                                   ggTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCa                                                                                    <  1:781710/140‑1 (MQ=255)                                                     tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCaaa                                                                                  >  1:460395/1‑140 (MQ=255)                                                     tACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCaaa                                                                                  >  1:467731/1‑140 (MQ=255)                                                       cGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGa                                                                                <  1:449752/140‑1 (MQ=255)                                                       cGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGa                                                                                <  1:342301/140‑1 (MQ=255)                                                          cGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGa                                                                             <  1:433616/140‑1 (MQ=255)                                                           gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:488662/140‑1 (MQ=255)                                                           gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:621990/140‑1 (MQ=255)                                                           gCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGAt                                                                            <  1:776845/140‑1 (MQ=255)                                                               ccGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGt                                                                        >  2:983163/1‑140 (MQ=255)                                                                 gTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGttt                                                                      >  2:232499/1‑140 (MQ=255)                                                                   gAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTAt                                                                    <  1:37530/140‑1 (MQ=255)                                                                     aTTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATgg                                                                  <  1:840126/140‑1 (MQ=255)                                                                       ttGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGat                                                                >  1:900789/1‑140 (MQ=255)                                                                         gACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGatca                                                              >  1:950388/1‑140 (MQ=255)                                                                               ctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGt                                                        >  1:703401/1‑140 (MQ=255)                                                                               ctcATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGt                                                        >  2:868631/1‑140 (MQ=255)                                                                                         ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  2:348525/1‑140 (MQ=255)                                                                                         ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  2:973039/1‑140 (MQ=255)                                                                                         ccAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGaaa                                              >  1:322189/1‑140 (MQ=255)                                                                                          cAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAAc                                             >  2:996365/1‑140 (MQ=255)                                                                                            gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCg                                           >  1:813251/1‑140 (MQ=255)                                                                                            gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCg                                           >  2:168046/1‑140 (MQ=255)                                                                                            gAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACACCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCTAAACCg                                           >  2:751103/1‑140 (MQ=255)                                                                                              aTTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGa                                         <  1:621089/140‑1 (MQ=255)                                                                                                   cGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGtc                                    <  1:348525/140‑1 (MQ=255)                                                                                                       tACGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTccc                                <  1:746827/140‑1 (MQ=255)                                                                                                         cGCCGACGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTccccc                              >  1:174189/1‑140 (MQ=255)                                                                                                              aCGCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAg                         >  2:852820/1‑140 (MQ=255)                                                                                                                gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:38543/140‑1 (MQ=255)                                                                                                                gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:393253/140‑1 (MQ=255)                                                                                                                gCTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:983163/140‑1 (MQ=255)                                                                                                                gCTATCGACTATTATAGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGt                       <  1:389798/140‑1 (MQ=255)                                                                                                                 cTATCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTg                      <  1:828151/140‑1 (MQ=255)                                                                                                                    tCGACTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGACAGTCTCCCCCCGCAGGCGGAg                   >  2:43659/1‑140 (MQ=255)                                                                                                                       aCTATTATCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTAt                <  2:703401/140‑1 (MQ=255)                                                                                                                         tattatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCa              >  2:791732/1‑140 (MQ=255)                                                                                                                         tattatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCa              <  1:716923/140‑1 (MQ=255)                                                                                                                          attatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCaa             <  1:868631/140‑1 (MQ=255)                                                                                                                           ttatCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAac            <  2:325059/140‑1 (MQ=255)                                                                                                                              tCGTCAGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCACAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAactga         >  1:124331/1‑140 (MQ=255)                                                                                                                                   aGTGTGGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGaa    >  1:327679/1‑140 (MQ=255)                                                                                                                                     tgtgGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa  >  1:706592/1‑140 (MQ=255)                                                                                                                                     tgtgGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACa  >  2:398989/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         CTGGCAGCCTACCCCGGAACGCTGCCGATTAATCTCGCGCAGTTAAAATCGGTACGTCGCATCCGTGAATTTGACGATCTCATCACCGCCAGAATTCACGGCTACGCCGACGCTATCGACTATTATCGTCAGTGTAGCGCCATGCCGATGCTGAACCGGATCGCCAAACCGACGCTGATTATTCACGCCAAAGACGATCCGTTTATGGATCATCAGGTGATCCCGAAACCGGAAAGTCTCCCCCCGCAGGTGGAGTATCAACTGACTGAACA  >  NC_000913/3483844‑3484115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑