Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,066	G→A	T420T (ACC→ACT)	ugpB ←	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,066	0	G	A	100.0%	158.5 / NA	46	T420T (ACC→ACT)	ugpB	sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/22);  total (24/22)

TGTGCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCGGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCTT  >  NC_000913/3590930‑3591191                                                                                                                                         |                                                                                                                              tgtgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTa                                                                                                                            >  2:481706/1‑140 (MQ=255)   tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:749805/1‑140 (MQ=255)   tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:733104/1‑140 (MQ=255)   tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:207507/1‑140 (MQ=255)   tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  1:26592/1‑140 (MQ=255)   tgCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATc                                                                                                                          >  2:508466/1‑140 (MQ=255)       ggATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGt                                                                                                                      <  1:883080/140‑1 (MQ=255)         aTGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGc                                                                                                                    <  2:783668/140‑1 (MQ=255)         aTGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGc                                                                                                                    <  2:749805/140‑1 (MQ=255)          tGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGcc                                                                                                                   <  2:689789/140‑1 (MQ=255)            cgcttcgcttATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCctg                                                                                                                 <  1:10901/140‑1 (MQ=255)                              tACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCCCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTAcc                                                                                               <  2:908325/140‑1 (MQ=255)                                cacGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCgg                                                                                             >  1:75217/1‑140 (MQ=255)                                      aTGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCcaca                                                                                       >  1:235573/1‑140 (MQ=255)                                        gCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACg                                                                                     <  2:207507/140‑1 (MQ=255)                                               cAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              <  2:973563/140‑1 (MQ=255)                                                 gATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGc                                                                            >  1:436849/1‑140 (MQ=255)                                                  aTAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGct                                                                           >  1:287481/1‑140 (MQ=255)                                                    aaGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGctct                                                                         <  2:131894/140‑1 (MQ=255)                                                     aGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCtt                                                                        <  2:235573/140‑1 (MQ=255)                                                      gCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTc                                                                       >  1:591013/1‑140 (MQ=255)                                                                tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              >  1:459346/1‑123 (MQ=255)                                                                tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCa                                                                              <  2:459346/123‑1 (MQ=255)                                                                tCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATc                                                             >  1:507048/1‑140 (MQ=255)                                                                   gACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCAcg                                                          <  2:733104/140‑1 (MQ=255)                                                                      aTTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCgg                                                       >  1:681241/1‑140 (MQ=255)                                                                      aTTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCgg                                                       >  1:867622/1‑140 (MQ=255)                                                                        tACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATTACGCGGAt                                                     <  1:508466/140‑1 (MQ=255)                                                                           acTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTg                                                  >  2:473830/1‑140 (MQ=255)                                                                               aTTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGc                                              >  1:637788/1‑140 (MQ=255)                                                                                ttAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCa                                             <  1:117906/140‑1 (MQ=255)                                                                                ttAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCa                                             >  1:40150/1‑140 (MQ=255)                                                                                   aGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGt                                          <  1:481706/140‑1 (MQ=255)                                                                                     aCTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTg                                        >  1:495355/1‑140 (MQ=255)                                                                                        tcgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGccc                                     <  2:235133/140‑1 (MQ=255)                                                                                         cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCa                                    >  2:565591/1‑140 (MQ=255)                                                                                         cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCa                                    >  1:184834/1‑140 (MQ=255)                                                                                         cgtcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGAGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCCGATCTGCGGCATGTTTcccc                                    >  2:415211/1‑139 (MQ=255)                                                                                           tcgATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGa                                  <  2:436849/140‑1 (MQ=255)                                                                                             gATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACg                                <  2:26592/140‑1 (MQ=255)                                                                                               tttCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCa                              <  2:591013/140‑1 (MQ=255)                                                                                                ttCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAg                             <  2:75217/140‑1 (MQ=255)                                                                                                 tctcAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGc                            <  2:442798/140‑1 (MQ=255)                                                                                                   tcAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGccc                          >  1:475361/1‑140 (MQ=255)                                                                                                           gcgcAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGa                  <  1:899645/140‑1 (MQ=255)                                                                                                           gcgcAGCAACTGATTTCCACGCTCAACGGAAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGa                  >  1:9636/1‑140 (MQ=255)                                                                                                            cgcAGCAACTGATTTCCACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGaa                 >  1:605282/1‑140 (MQ=255)                                                                                                                           ccACGCTCAACGGCAGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCtt  >  2:392819/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                              TGTGCCGGATGCGCTTCGCTTATCCGGCCTACACGCGATGCCAGGTCAGATAAGCGAAACGCATCCGACATTACACTGATTAAGACTTCGTCGATTTCTCAAAGCGGCGCAGCAACTGATTTCCACGCTCAACGGCGGTATCCAGTGCCTGCTGTGGTGTCTTCTTACCGGTCCACACGCTCTCCAGCTCTTCATCCACAATCACGCGGATCTGCGGCATGTTGCCCAGACGCAGCCCTTTGGTGAACGGCAACGGCGGCTT  >  NC_000913/3590930‑3591191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑