Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,972	T→C	A45A (GCT→GCC)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,972	0	T	C	100.0%	204.6 / NA	55	A45A (GCT→GCC)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/25);  total (30/25)

CCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGC  >  NC_000913/3786836‑3787102                                                                                                                                         |                                                                                                                                   ccATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGAc                                                                                                                                 >  1:955661/1‑140 (MQ=255)   aTGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACAt                                                                                                                               >  2:343228/1‑140 (MQ=255)     gACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATcg                                                                                                                             >  1:537771/1‑140 (MQ=255)         cGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATcgccgc                                                                                                                         >  2:80872/1‑140 (MQ=255)            gCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCaaa                                                                                                                      >  1:803465/1‑140 (MQ=255)            gCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCaaa                                                                                                                      >  1:24382/1‑140 (MQ=255)            gCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCaaa                                                                                                                      >  1:270208/1‑140 (MQ=255)               gTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAaga                                                                                                                   >  1:119392/1‑140 (MQ=255)                  aaaCCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAgcg                                                                                                                <  2:513123/140‑1 (MQ=255)                    agcgcgCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAgcgcg                                                                                                              <  2:626731/138‑1 (MQ=255)                    aCCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAgcgcg                                                                                                              <  2:955661/140‑1 (MQ=255)                      cgcgCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGcgcgcg                                                                                                            <  2:458635/140‑1 (MQ=255)                                tGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCaaa                                                                                                  <  1:143486/140‑1 (MQ=255)                                tGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCaaa                                                                                                  <  1:854807/140‑1 (MQ=255)                                       aGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaaca                                                                                           >  2:398822/1‑140 (MQ=255)                                        ggCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaa                                                                                          >  1:86280/1‑140 (MQ=255)                                         gCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaac                                                                                         <  2:390507/140‑1 (MQ=255)                                          cccATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaaca                                                                                        <  2:638230/140‑1 (MQ=255)                                          cccATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaaca                                                                                        <  2:119392/140‑1 (MQ=255)                                          cccATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaaca                                                                                        <  2:634948/140‑1 (MQ=255)                                          cccATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGcaacaaca                                                                                        <  2:65429/140‑1 (MQ=255)                                               catcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGCACGCCAAAAGCAACAACAAcgcg                                                                                   >  1:860591/1‑140 (MQ=255)                                                     cGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGcc                                                                             >  1:49238/1‑140 (MQ=255)                                                       ccAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTg                                                                           >  1:703174/1‑140 (MQ=255)                                                           cGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGTAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                                                                       <  1:96019/140‑1 (MQ=255)                                                           cGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                                                                       >  1:647924/1‑140 (MQ=255)                                                           cGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                                                                       >  1:537468/1‑140 (MQ=255)                                                            gTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGc                                                                      <  1:405397/140‑1 (MQ=255)                                                             ttCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGca                                                                     <  1:80872/140‑1 (MQ=255)                                                                  aGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAAtt                                                                >  1:856447/1‑140 (MQ=255)                                                                     gcTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGaa                                                             >  2:203536/1‑140 (MQ=255)                                                                      cTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGaaa                                                            >  1:442225/1‑140 (MQ=255)                                                                        ggCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGaaaaa                                                          <  2:814262/140‑1 (MQ=255)                                                                         gCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAg                                                         >  1:991544/1‑140 (MQ=255)                                                                         gCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAg                                                         >  1:3683/1‑140 (MQ=255)                                                                                 ttgtGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGgaaga                                                 >  2:363865/1‑140 (MQ=255)                                                                                    tgCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGc                                              >  1:160349/1‑140 (MQ=255)                                                                                     gcgcCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCg                                             <  2:86280/140‑1 (MQ=255)                                                                                     gcgcCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCg                                             <  2:648326/140‑1 (MQ=255)                                                                                     gcgcCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCg                                             >  1:754737/1‑140 (MQ=255)                                                                                      cgcCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGa                                            >  2:590255/1‑140 (MQ=255)                                                                                        ccTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAtc                                          >  2:674786/1‑140 (MQ=255)                                                                                         cTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAtct                                         >  1:1008811/1‑140 (MQ=255)                                                                                                gCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGc                                  <  1:794633/140‑1 (MQ=255)                                                                                                gCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGc                                  <  1:28650/140‑1 (MQ=255)                                                                                                gCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGc                                  <  1:198245/140‑1 (MQ=255)                                                                                                     cgcgGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCAccc                             >  1:210878/1‑140 (MQ=255)                                                                                                     cgcgGATGAGCGTGACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCAccc                             >  2:89193/1‑140 (MQ=255)                                                                                                           cgAGCGTGACAAACTCAAATCTATTGAGGCCGACACCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCGCCCGTAAGc                       <  2:510121/139‑1 (MQ=255)                                                                                                                  gACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGa                <  1:398822/140‑1 (MQ=255)                                                                                                                  gACCAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGa                >  2:677440/1‑140 (MQ=255)                                                                                                                     cAACTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAAc             <  1:426640/140‑1 (MQ=255)                                                                                                                        cTCAAATCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGACGCCACCCGTAAGCTGCGCGAAACGCa          >  2:637934/1‑140 (MQ=255)                                                                                                                           aaaTCTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCaaaa       <  2:24382/140‑1 (MQ=255)                                                                                                                               cTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACg   <  2:991544/140‑1 (MQ=255)                                                                                                                               cTATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACg   <  2:647924/140‑1 (MQ=255)                                                                                                                                tATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGc  <  2:860591/140‑1 (MQ=255)                                                                                                                                tATTCAGGCCGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGc  <  2:1008811/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGC  >  NC_000913/3786836‑3787102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑