Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,867,173	T→C	T84A (ACC→GCC)	ibpA ←	small heat shock protein IbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,867,173	0	T	C	100.0%	244.0 / NA	67	T84A (ACC→GCC)	ibpA	small heat shock protein IbpA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/23);  total (44/23)

TTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAA  >  NC_000913/3867037‑3867307                                                                                                                                         |                                                                                                                                       ttCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGcgcg                                                                                                                                     <  1:23632/140‑1 (MQ=255)  tCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGgcgcgc                                                                                                                                    <  1:387546/140‑1 (MQ=255)                  gcgTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCgg                                                                                                                    >  1:687805/1‑140 (MQ=255)                   cgTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCATTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGc                                                                                                                   <  2:345996/140‑1 (MQ=255)                        gagaTCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAg                                                                                                              <  2:93054/140‑1 (MQ=255)                         agaTCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGc                                                                                                             <  2:556027/140‑1 (MQ=255)                                     aGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTcaccac                                                                                                 >  1:423451/1‑140 (MQ=255)                                      gCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCaccacc                                                                                                >  2:430607/1‑140 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCAccacca                                                                                               >  2:960149/1‑140 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCAccacca                                                                                               >  1:632845/1‑140 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCAccacca                                                                                               >  1:787287/1‑140 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCAccacca                                                                                               >  1:98394/1‑140 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCAccacca                                                                                               <  2:31060/140‑1 (MQ=255)                                       cAAACCATTTACCAGGTTAGCACCACAAACTTGAATGTTCTCAGCTAACTGGAATATGCGTTCAAAGTTCCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGACCACCTTTCAccacca                                                                                               >  2:239040/1‑140 (MQ=255)                                        aaaCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcag                                                                                              >  1:714057/1‑140 (MQ=255)                                         aaCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagc                                                                                             >  1:597545/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:1001091/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:882775/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  2:859871/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:781607/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  2:95381/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:922283/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:60706/1‑140 (MQ=255)                                          aCCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACcagca                                                                                            >  1:171577/1‑140 (MQ=255)                                            cATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGa                                                                                          >  1:585526/1‑140 (MQ=255)                                            cATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGa                                                                                          <  2:245359/140‑1 (MQ=255)                                               ttACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTa                                                                                       >  1:802941/1‑140 (MQ=255)                                                      gTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTggg                                                                                >  2:34240/1‑140 (MQ=255)                                                           caccacGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTa                                                                           >  1:318536/1‑140 (MQ=255)                                                           caccacGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTa                                                                           >  2:61565/1‑140 (MQ=255)                                                                cGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTc                                                                      >  2:13532/1‑140 (MQ=255)                                                                cGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTc                                                                      >  1:972853/1‑140 (MQ=255)                                                                cGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTc                                                                      >  1:342923/1‑140 (MQ=255)                                                                cGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTc                                                                      >  2:673392/1‑140 (MQ=255)                                                                        aaTGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGc                                                              >  2:229770/1‑140 (MQ=255)                                                                          tGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGctc                                                            <  1:948324/140‑1 (MQ=255)                                                                             tctcAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCa                                                         >  1:453092/1‑140 (MQ=255)                                                                             tctcAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCa                                                         >  1:1030942/1‑140 (MQ=255)                                                                              ctcAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAg                                                        <  2:650944/140‑1 (MQ=255)                                                                               tcAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGc                                                       >  1:920189/1‑140 (MQ=255)                                                                                cAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCa                                                      <  2:562232/140‑1 (MQ=255)                                                                                     aaCTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAAcc                                                 <  1:585115/140‑1 (MQ=255)                                                                                      aCTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCa                                                >  1:101894/1‑140 (MQ=255)                                                                                        tGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGc                                              >  1:866103/1‑140 (MQ=255)                                                                                           aaTTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCcac                                           >  2:103195/1‑140 (MQ=255)                                                                                           aaTTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCcac                                           >  1:686997/1‑140 (MQ=255)                                                                                           aaTTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCcac                                           >  1:309928/1‑140 (MQ=255)                                                                                             tttGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAg                                         >  1:282924/1‑140 (MQ=255)                                                                                              ttGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGc                                        <  1:564316/140‑1 (MQ=255)                                                                                                      aaaGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCaa                                <  2:656275/140‑1 (MQ=255)                                                                                                       aaGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAAt                               <  2:375502/140‑1 (MQ=255)                                                                                                        aGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATg                              <  2:781607/140‑1 (MQ=255)                                                                                                        aGTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATg                              <  2:632845/140‑1 (MQ=255)                                                                                                         gTTGCGTTCAGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGc                             <  2:802941/140‑1 (MQ=255)                                                                                                                  aGCGATGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGt                    >  1:192731/1‑140 (MQ=255)                                                                                                                       tGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCg               >  1:692901/1‑140 (MQ=255)                                                                                                                       tGCCCTGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCg               <  2:14580/140‑1 (MQ=255)                                                                                                                            tGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTAc          <  2:922283/140‑1 (MQ=255)                                                                                                                            tGGTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTAc          <  1:430607/140‑1 (MQ=255)                                                                                                                              gTACAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCa        >  2:328351/1‑140 (MQ=255)                                                                                                                                 cAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGtt     <  1:328351/140‑1 (MQ=255)                                                                                                                                 cAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGtt     <  2:1001091/140‑1 (MQ=255)                                                                                                                                 cAGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGtt     <  1:859871/140‑1 (MQ=255)                                                                                                                                 aaGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGtt     <  2:588155/139‑1 (MQ=255)                                                                                                                                  aGATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTc    <  1:229770/140‑1 (MQ=255)                                                                                                                                   gagaGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCa   <  2:60706/137‑1 (MQ=255)                                                                                                                                   gATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCa   <  1:13532/140‑1 (MQ=255)                                                                                                                                   gATAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCa   >  1:591121/1‑140 (MQ=255)                                                                                                                                    aTAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCaa  >  2:667912/1‑140 (MQ=255)                                                                                                                                    aTAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCaa  >  2:587900/1‑140 (MQ=255)                                                                                                                                    aTAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCaa  >  1:411667/1‑140 (MQ=255)                                                                                                                                    aTAGGCGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCaa  >  2:250192/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTCGCTTCCGGAATCACGCGTTCGAGATCGATATACAGCAAACCATTTACCAGGTTAGCACCACGAACATGAATGTTCTCAGCTAACTGGAATTTGCGTTCAAAGTTGCGTTCAGCGATGCCCTGGTACAGATAGGTGCGCTCTTTTTGTTCGTCGGCGTGAGCACCTTTCACCACCAGCAGATTATCCTGGGCGGTAATTTCCAGTTCGCTCTCAGCAAAACCAGCCACAGCGATAGCAATGCGGTAATGGTTTTCGTCTACCAGTTCAA  >  NC_000913/3867037‑3867307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑