Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,248	A→G	T216A (ACA→GCA)	insI‑1 →	IS30 transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,248	0	A	G	100.0%	87.9 / NA	40	T216A (ACA→GCA)	insI‑1	IS30 transposase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/22);  total (18/22)

TATACAGCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAACACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGATCA  >  NC_000913/271157‑271380                                                                                            |                                                                                                                                     tataCAGCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTgg                                                                                      >  1:987317/1‑140 (MQ=14)     cAGCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCa                                                                                  >  1:688648/1‑140 (MQ=17)      aGCATCTGCGACGGTCGCATAGCCTTCGTCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCAt                                                                                 >  1:579837/1‑140 (MQ=12)       gCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCAtt                                                                                >  1:298578/1‑140 (MQ=17)         aTCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTgg                                                                              >  1:1027293/1‑140 (MQ=17)         aTCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTgg                                                                              >  1:916254/1‑140 (MQ=17)         aTCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTgg                                                                              >  2:611634/1‑140 (MQ=17)         aTCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTgg                                                                              >  1:404317/1‑140 (MQ=17)          tCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTggg                                                                             >  1:596642/1‑140 (MQ=17)          tCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTggg                                                                             >  2:443860/1‑140 (MQ=17)           cTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGa                                                                            <  1:214866/140‑1 (MQ=18)             gCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAgg                                                                          <  2:344166/140‑1 (MQ=18)             gCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAgg                                                                          <  1:863211/140‑1 (MQ=18)                          aGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGtctc                                                             <  2:979382/140‑1 (MQ=18)                           gCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCa                                                            <  1:706836/140‑1 (MQ=17)                              ttCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGt                                                         <  2:612191/140‑1 (MQ=18)                              ttCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGt                                                         >  2:501005/1‑140 (MQ=17)                                      ggCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACaaaaaa                                                 <  1:443860/140‑1 (MQ=18)                                      ggCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACaaaaaa                                                 <  1:814580/140‑1 (MQ=18)                                      ggCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCAATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACaaaaaa                                                 >  2:828090/1‑140 (MQ=17)                                       gCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAAc                                                <  1:1018205/140‑1 (MQ=18)                                        cAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAAct                                               <  1:160768/140‑1 (MQ=18)                                           gCGTCATACCCGCAAAGGCGAAAGAGGCACGATTAACATAGCGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGCGGGCGATTTAGCCTCAGGTACAAAAAACtctc                                            >  2:582880/1‑140 (MQ=11)                                            cGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGTGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCa                                           <  2:343140/140‑1 (MQ=16)                                            cGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCa                                           <  1:1035248/140‑1 (MQ=18)                                                  aCCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGc                                     <  1:946688/140‑1 (MQ=17)                                                  aCCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGc                                     <  1:758685/140‑1 (MQ=17)                                                    ccGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCca                                   <  2:404317/140‑1 (MQ=17)                                                    ccGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCca                                   <  2:688648/140‑1 (MQ=17)                                                    ccGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCca                                   <  2:987317/140‑1 (MQ=17)                                                                      tACGATTAACATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAAt                 <  1:410510/140‑1 (MQ=17)                                                                             aaCATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTta          >  1:457963/1‑140 (MQ=17)                                                                             aaCATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTta          >  2:974586/1‑140 (MQ=17)                                                                               cATAGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTtata        <  1:611634/140‑1 (MQ=17)                                                                                  aGTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGa     >  2:928141/1‑140 (MQ=17)                                                                                   gTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGat    <  2:840715/140‑1 (MQ=17)                                                                                   gTGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGat    <  2:947160/140‑1 (MQ=17)                                                                                    tGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGatc   >  2:21769/1‑140 (MQ=17)                                                                                    tGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGatc   >  1:286533/1‑140 (MQ=17)                                                                                    tGAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGatc   >  2:998522/1‑140 (MQ=17)                                                                                     gAACGGAGCACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGatca  <  2:515781/140‑1 (MQ=17)                                                                                            |                                                                                                                                     TATACAGCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGAACGGAACACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCAGGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGATCA  >  NC_000913/271157‑271380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑